Nanodrug‐Engineered Exosomes Achieve a Jointly Dual‐Pathway Inhibition on Cuproptosis
Abstract Cuproptosis, caused by an intracellular overload of copper (Cu) ions and overexpression of ferredoxin 1 (FDX1), is identified for its regulatory role in the skin wound healing process. This study verifies the presence of cuproptosis in skin wound beds and reactive oxygen species‐induced cel...
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| Format: | Article |
| Language: | English |
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Wiley
2025-01-01
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| Series: | Advanced Science |
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| Online Access: | https://doi.org/10.1002/advs.202413408 |
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| author | Hanxiao Sun Yang Zou Zhengtai Chen Yan He Kai Ye Huan Liu Lihong Qiu Yufan Zhang Yuexue Mai Xinghong Chen Zhengwei Mao Wei Wang Chenggang Yi |
| author_facet | Hanxiao Sun Yang Zou Zhengtai Chen Yan He Kai Ye Huan Liu Lihong Qiu Yufan Zhang Yuexue Mai Xinghong Chen Zhengwei Mao Wei Wang Chenggang Yi |
| author_sort | Hanxiao Sun |
| collection | DOAJ |
| description | Abstract Cuproptosis, caused by an intracellular overload of copper (Cu) ions and overexpression of ferredoxin 1 (FDX1), is identified for its regulatory role in the skin wound healing process. This study verifies the presence of cuproptosis in skin wound beds and reactive oxygen species‐induced cells model. To address the two pathways leading to cell cuproptosis, a nanodrug‐engineered exosomes is proposed. A Cu‐chelator (Clioquinol, CQ) polydopamine (PDA)‐modified stem cell exosome loaded with siRNA‐FDX1, named EXOsiFDX1‐PDA@CQ, is designed to efficiently inhibit the two cuproptosis pathways. The functionalized exosomes are loaded into an injectable hydrogel and applied to treat diabetic wounds in mice and acute skin wounds in pigs. The local and controlled release of EXOsiFDX1‐PDA@CQ ensures the retention of the therapeutic agent at wound beds, effectively promoting wound healing. The strategy of engineered exosomes with functional nanoparticles (NPs) proposed in this study offers an efficient and scalable new approach for regulating cuproptosis. |
| format | Article |
| id | doaj-art-c0e6cc37c1c34fe1a988d7dc75061679 |
| institution | Kabale University |
| issn | 2198-3844 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Advanced Science |
| spelling | doaj-art-c0e6cc37c1c34fe1a988d7dc750616792025-01-29T09:50:19ZengWileyAdvanced Science2198-38442025-01-01124n/an/a10.1002/advs.202413408Nanodrug‐Engineered Exosomes Achieve a Jointly Dual‐Pathway Inhibition on CuproptosisHanxiao Sun0Yang Zou1Zhengtai Chen2Yan He3Kai Ye4Huan Liu5Lihong Qiu6Yufan Zhang7Yuexue Mai8Xinghong Chen9Zhengwei Mao10Wei Wang11Chenggang Yi12The Second Affiliated Hospital of Zhejiang University College of Medicine Hangzhou 310000 ChinaCollege of Chemical and Biological Engineering Zhejiang University Hangzhou Zhejiang 310027 ChinaThe Second Affiliated Hospital of Zhejiang University College of Medicine Hangzhou 310000 ChinaThe Second Affiliated Hospital of Zhejiang University College of Medicine Hangzhou 310000 ChinaThe Second Affiliated Hospital of Zhejiang University College of Medicine Hangzhou 310000 ChinaThe Second Affiliated Hospital of Zhejiang University College of Medicine Hangzhou 310000 ChinaThe Second Affiliated Hospital of Zhejiang University College of Medicine Hangzhou 310000 ChinaThe Second Affiliated Hospital of Zhejiang University College of Medicine Hangzhou 310000 ChinaThe Second Affiliated Hospital of Zhejiang University College of Medicine Hangzhou 310000 ChinaThe Second Affiliated Hospital of Zhejiang University College of Medicine Hangzhou 310000 ChinaMOE Key Laboratory of Macromolecular Synthesis and Functionalization Department of Polymer Science and Engineering Zhejiang University Hangzhou Zhejiang 310027 ChinaThe Second Affiliated Hospital of Zhejiang University College of Medicine Hangzhou 310000 ChinaThe Second Affiliated Hospital of Zhejiang University College of Medicine Hangzhou 310000 ChinaAbstract Cuproptosis, caused by an intracellular overload of copper (Cu) ions and overexpression of ferredoxin 1 (FDX1), is identified for its regulatory role in the skin wound healing process. This study verifies the presence of cuproptosis in skin wound beds and reactive oxygen species‐induced cells model. To address the two pathways leading to cell cuproptosis, a nanodrug‐engineered exosomes is proposed. A Cu‐chelator (Clioquinol, CQ) polydopamine (PDA)‐modified stem cell exosome loaded with siRNA‐FDX1, named EXOsiFDX1‐PDA@CQ, is designed to efficiently inhibit the two cuproptosis pathways. The functionalized exosomes are loaded into an injectable hydrogel and applied to treat diabetic wounds in mice and acute skin wounds in pigs. The local and controlled release of EXOsiFDX1‐PDA@CQ ensures the retention of the therapeutic agent at wound beds, effectively promoting wound healing. The strategy of engineered exosomes with functional nanoparticles (NPs) proposed in this study offers an efficient and scalable new approach for regulating cuproptosis.https://doi.org/10.1002/advs.202413408cuproptosisengineered exosomesFDX1wound healing |
| spellingShingle | Hanxiao Sun Yang Zou Zhengtai Chen Yan He Kai Ye Huan Liu Lihong Qiu Yufan Zhang Yuexue Mai Xinghong Chen Zhengwei Mao Wei Wang Chenggang Yi Nanodrug‐Engineered Exosomes Achieve a Jointly Dual‐Pathway Inhibition on Cuproptosis Advanced Science cuproptosis engineered exosomes FDX1 wound healing |
| title | Nanodrug‐Engineered Exosomes Achieve a Jointly Dual‐Pathway Inhibition on Cuproptosis |
| title_full | Nanodrug‐Engineered Exosomes Achieve a Jointly Dual‐Pathway Inhibition on Cuproptosis |
| title_fullStr | Nanodrug‐Engineered Exosomes Achieve a Jointly Dual‐Pathway Inhibition on Cuproptosis |
| title_full_unstemmed | Nanodrug‐Engineered Exosomes Achieve a Jointly Dual‐Pathway Inhibition on Cuproptosis |
| title_short | Nanodrug‐Engineered Exosomes Achieve a Jointly Dual‐Pathway Inhibition on Cuproptosis |
| title_sort | nanodrug engineered exosomes achieve a jointly dual pathway inhibition on cuproptosis |
| topic | cuproptosis engineered exosomes FDX1 wound healing |
| url | https://doi.org/10.1002/advs.202413408 |
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