Functional Verification of Differentially Expressed Genes Following DENV2 Infection in <i>Aedes aegypti</i>
The dengue virus (DENV) is primarily transmitted by <i>Aedes aegypti</i>. Investigating genes associated with mosquito susceptibility to DENV2 offers a theoretical foundation for targeted interventions to regulate or block viral replication and transmission within mosquitoes. Based on th...
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2025-01-01
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| author | Xiaoli Chen Xinyu Zhou Xiaoxue Xie Bo Li Teng Zhao Haotian Yu Dan Xing Jiahong Wu Chunxiao Li |
| author_facet | Xiaoli Chen Xinyu Zhou Xiaoxue Xie Bo Li Teng Zhao Haotian Yu Dan Xing Jiahong Wu Chunxiao Li |
| author_sort | Xiaoli Chen |
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| description | The dengue virus (DENV) is primarily transmitted by <i>Aedes aegypti</i>. Investigating genes associated with mosquito susceptibility to DENV2 offers a theoretical foundation for targeted interventions to regulate or block viral replication and transmission within mosquitoes. Based on the transcriptomic analyses of the midgut and salivary glands from <i>Aedes aegypti</i> infected with DENV2, alongside analyses of Aag2 cell infections, 24 genes potentially related to the regulation of <i>Aedes aegypti</i> infection with DENV2 were selected. By establishing transient transfection and overexpression models of <i>Aedes aegypti</i> Aag2 cells, and mosquito target gene interference models, the difference in viral load before and after treatment was compared, and the effects of DEGs on viral replication were evaluated. After overexpressing 24 DEGs in Aag2 cells, 19 DEGs showed a significant difference in DENV2 RNA copies in the cell supernatant (<i>p</i> < 0.05). In adult mosquitoes, knocking down defensin-A, defensin-A-like, and SMCT1 respectively reduced the DENV2 RNA copies, while knocking down UGT2B1 and ND4 respectively increased the DENV2 RNA copies. In this study, to assess the role of genes related to DENV2 replication, and transient transfection and overexpression models in Aag2 cells and mosquito gene knockdown models were established, and five genes, defensin-A, defensin-A-like, SMCT1, UGT2B1, and ND4, were found to have an impact on the replication of DENV2, providing a reference basis for studying the complex mechanism of mosquito–virus interactions. |
| format | Article |
| id | doaj-art-c0df0c9d519347e5a836348d47f08459 |
| institution | Kabale University |
| issn | 1999-4915 |
| language | English |
| publishDate | 2025-01-01 |
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| series | Viruses |
| spelling | doaj-art-c0df0c9d519347e5a836348d47f084592025-01-24T13:52:28ZengMDPI AGViruses1999-49152025-01-011716710.3390/v17010067Functional Verification of Differentially Expressed Genes Following DENV2 Infection in <i>Aedes aegypti</i>Xiaoli Chen0Xinyu Zhou1Xiaoxue Xie2Bo Li3Teng Zhao4Haotian Yu5Dan Xing6Jiahong Wu7Chunxiao Li8The Key Laboratory of Environmental Pollution Monitoring and Disease Control, School of Public Health, Ministry of Education, Guizhou Medical University, Guiyang 550025, ChinaState Key Laboratory of Pathogen and Biosecurity, Beijing 100071, ChinaState Key Laboratory of Pathogen and Biosecurity, Beijing 100071, ChinaState Key Laboratory of Pathogen and Biosecurity, Beijing 100071, ChinaState Key Laboratory of Pathogen and Biosecurity, Beijing 100071, ChinaState Key Laboratory of Pathogen and Biosecurity, Beijing 100071, ChinaState Key Laboratory of Pathogen and Biosecurity, Beijing 100071, ChinaThe Key and Characteristic Laboratory of Modern Pathogen Biology, College of Basic Medicine, Guizhou Medical University, Guiyang 550025, ChinaState Key Laboratory of Pathogen and Biosecurity, Beijing 100071, ChinaThe dengue virus (DENV) is primarily transmitted by <i>Aedes aegypti</i>. Investigating genes associated with mosquito susceptibility to DENV2 offers a theoretical foundation for targeted interventions to regulate or block viral replication and transmission within mosquitoes. Based on the transcriptomic analyses of the midgut and salivary glands from <i>Aedes aegypti</i> infected with DENV2, alongside analyses of Aag2 cell infections, 24 genes potentially related to the regulation of <i>Aedes aegypti</i> infection with DENV2 were selected. By establishing transient transfection and overexpression models of <i>Aedes aegypti</i> Aag2 cells, and mosquito target gene interference models, the difference in viral load before and after treatment was compared, and the effects of DEGs on viral replication were evaluated. After overexpressing 24 DEGs in Aag2 cells, 19 DEGs showed a significant difference in DENV2 RNA copies in the cell supernatant (<i>p</i> < 0.05). In adult mosquitoes, knocking down defensin-A, defensin-A-like, and SMCT1 respectively reduced the DENV2 RNA copies, while knocking down UGT2B1 and ND4 respectively increased the DENV2 RNA copies. In this study, to assess the role of genes related to DENV2 replication, and transient transfection and overexpression models in Aag2 cells and mosquito gene knockdown models were established, and five genes, defensin-A, defensin-A-like, SMCT1, UGT2B1, and ND4, were found to have an impact on the replication of DENV2, providing a reference basis for studying the complex mechanism of mosquito–virus interactions.https://www.mdpi.com/1999-4915/17/1/67<i>Aedes aegypti</i>Aag2 cellsDENV2overexpressionsiRNA |
| spellingShingle | Xiaoli Chen Xinyu Zhou Xiaoxue Xie Bo Li Teng Zhao Haotian Yu Dan Xing Jiahong Wu Chunxiao Li Functional Verification of Differentially Expressed Genes Following DENV2 Infection in <i>Aedes aegypti</i> Viruses <i>Aedes aegypti</i> Aag2 cells DENV2 overexpression siRNA |
| title | Functional Verification of Differentially Expressed Genes Following DENV2 Infection in <i>Aedes aegypti</i> |
| title_full | Functional Verification of Differentially Expressed Genes Following DENV2 Infection in <i>Aedes aegypti</i> |
| title_fullStr | Functional Verification of Differentially Expressed Genes Following DENV2 Infection in <i>Aedes aegypti</i> |
| title_full_unstemmed | Functional Verification of Differentially Expressed Genes Following DENV2 Infection in <i>Aedes aegypti</i> |
| title_short | Functional Verification of Differentially Expressed Genes Following DENV2 Infection in <i>Aedes aegypti</i> |
| title_sort | functional verification of differentially expressed genes following denv2 infection in i aedes aegypti i |
| topic | <i>Aedes aegypti</i> Aag2 cells DENV2 overexpression siRNA |
| url | https://www.mdpi.com/1999-4915/17/1/67 |
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