Comparative Study of Antioxidant Status in Androgenic Embryos of Aesculus hippocastanum and Aesculus flava
In vivo (leaves and seed embryos) and in vitro (androgenic embryos) antioxidant scavenging activity of Aesculus hippocastanum and Aesculus flava medical plants was examined. Here we report antioxidant enzyme activities of superoxide dismutase, catalase, guaiacol peroxidase and glutathione peroxidase...
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| Format: | Article |
| Language: | English |
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Wiley
2014-01-01
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| Series: | The Scientific World Journal |
| Online Access: | http://dx.doi.org/10.1155/2014/767392 |
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| author | Dubravka Štajner Boris M. Popović Dušica Ćalić Marijana Štajner |
| author_facet | Dubravka Štajner Boris M. Popović Dušica Ćalić Marijana Štajner |
| author_sort | Dubravka Štajner |
| collection | DOAJ |
| description | In vivo (leaves and seed embryos) and in vitro (androgenic embryos) antioxidant scavenging activity of Aesculus hippocastanum and Aesculus flava medical plants was examined. Here we report antioxidant enzyme activities of superoxide dismutase, catalase, guaiacol peroxidase and glutathione peroxidase, reduced glutathione quantity, flavonoids, soluble protein contents, quantities of malondialdehyde, and •OH radical presence in the investigated plant samples. Total antioxidant capacity of all the samples of A. hippocastanum and A. flava was determined using FRAP, DPPH, and NO• radical scavenger capacity. The leaves of A. flava collected from the botanical garden exhibited stronger antioxidant activity (higher activities of SOD, and higher quantities of GSH, TSH, TPC, and scavenging abilities of DPPH and NO•, and higher FRAP values and lowest quantities of •OH and MDA) than in vitro obtained cultures. However, the leaves of A. flava showed higher antioxidant activity than the leaves of A. hippocastanum, and therefore they have a stronger tolerance of oxidative stress. Androgenic embryos of both species had low amount of antioxidants due to controlled in vitro environmental conditions (T, photoperiod, humidity, nutritive factors, and pathogen-free). Our results confirmed that we found optimal in vitro conditions for producing androgenic embryos of both Aesculus species. Also, we assume that horse chestnut androgenic embryos can be used as an alternative source for large-scale aescin production. |
| format | Article |
| id | doaj-art-c0ddd32c198d4289a00c3a11d07247e5 |
| institution | Kabale University |
| issn | 2356-6140 1537-744X |
| language | English |
| publishDate | 2014-01-01 |
| publisher | Wiley |
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| series | The Scientific World Journal |
| spelling | doaj-art-c0ddd32c198d4289a00c3a11d07247e52025-02-03T05:43:54ZengWileyThe Scientific World Journal2356-61401537-744X2014-01-01201410.1155/2014/767392767392Comparative Study of Antioxidant Status in Androgenic Embryos of Aesculus hippocastanum and Aesculus flavaDubravka Štajner0Boris M. Popović1Dušica Ćalić2Marijana Štajner3Faculty of Agriculture, University of Novi Sad, Trg Dositeja Obradovića 8, 21 000 Novi Sad, SerbiaFaculty of Agriculture, University of Novi Sad, Trg Dositeja Obradovića 8, 21 000 Novi Sad, SerbiaDepartment of Plant Physiology, Institute for Biological Research “Siniša Stanković,” University of Belgrade, Despota Stefana Boulevard 142, 11000 Belgrade, SerbiaEmergency Centre, Clinical Centre of Vojvodina, Hajduk Veljkova 1, 21000 Novi Sad, SerbiaIn vivo (leaves and seed embryos) and in vitro (androgenic embryos) antioxidant scavenging activity of Aesculus hippocastanum and Aesculus flava medical plants was examined. Here we report antioxidant enzyme activities of superoxide dismutase, catalase, guaiacol peroxidase and glutathione peroxidase, reduced glutathione quantity, flavonoids, soluble protein contents, quantities of malondialdehyde, and •OH radical presence in the investigated plant samples. Total antioxidant capacity of all the samples of A. hippocastanum and A. flava was determined using FRAP, DPPH, and NO• radical scavenger capacity. The leaves of A. flava collected from the botanical garden exhibited stronger antioxidant activity (higher activities of SOD, and higher quantities of GSH, TSH, TPC, and scavenging abilities of DPPH and NO•, and higher FRAP values and lowest quantities of •OH and MDA) than in vitro obtained cultures. However, the leaves of A. flava showed higher antioxidant activity than the leaves of A. hippocastanum, and therefore they have a stronger tolerance of oxidative stress. Androgenic embryos of both species had low amount of antioxidants due to controlled in vitro environmental conditions (T, photoperiod, humidity, nutritive factors, and pathogen-free). Our results confirmed that we found optimal in vitro conditions for producing androgenic embryos of both Aesculus species. Also, we assume that horse chestnut androgenic embryos can be used as an alternative source for large-scale aescin production.http://dx.doi.org/10.1155/2014/767392 |
| spellingShingle | Dubravka Štajner Boris M. Popović Dušica Ćalić Marijana Štajner Comparative Study of Antioxidant Status in Androgenic Embryos of Aesculus hippocastanum and Aesculus flava The Scientific World Journal |
| title | Comparative Study of Antioxidant Status in Androgenic Embryos of Aesculus hippocastanum and Aesculus flava |
| title_full | Comparative Study of Antioxidant Status in Androgenic Embryos of Aesculus hippocastanum and Aesculus flava |
| title_fullStr | Comparative Study of Antioxidant Status in Androgenic Embryos of Aesculus hippocastanum and Aesculus flava |
| title_full_unstemmed | Comparative Study of Antioxidant Status in Androgenic Embryos of Aesculus hippocastanum and Aesculus flava |
| title_short | Comparative Study of Antioxidant Status in Androgenic Embryos of Aesculus hippocastanum and Aesculus flava |
| title_sort | comparative study of antioxidant status in androgenic embryos of aesculus hippocastanum and aesculus flava |
| url | http://dx.doi.org/10.1155/2014/767392 |
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