First Report on Microbial-Derived Polydeoxyribonucleotide: A Sustainable and Enhanced Alternative to Salmon-Based Polydeoxyribonucleotide
Polydeoxyribonucleotide (PDRN) has emerged as a potent bioactive compound with proven efficacy in wound healing, tissue regeneration, and anti-inflammatory applications and is predominantly derived from salmonid gonads. However, this study presents a groundbreaking advancement by successfully extrac...
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2025-01-01
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| author | Doobyeong Chae Sae-Woong Oh Yoon-Seo Choi Dae-Jung Kang Chun-Woong Park Jongsung Lee Won-Sang Seo |
| author_facet | Doobyeong Chae Sae-Woong Oh Yoon-Seo Choi Dae-Jung Kang Chun-Woong Park Jongsung Lee Won-Sang Seo |
| author_sort | Doobyeong Chae |
| collection | DOAJ |
| description | Polydeoxyribonucleotide (PDRN) has emerged as a potent bioactive compound with proven efficacy in wound healing, tissue regeneration, and anti-inflammatory applications and is predominantly derived from salmonid gonads. However, this study presents a groundbreaking advancement by successfully extracting and characterizing PDRN from microbial sources, specifically <i>Lactobacillus rhamnosus</i>, marking the first report to utilize microbial-, biome-, or <i>Lactobacillus</i>-derived PDRN (L-PDRN). The findings demonstrate the enhanced biological properties of L-PDRN over traditional salmon-derived PDRN across several assays. L-PDRN exhibited superior antioxidant activity, with significantly higher SOD-like and DPPH radical scavenging activities compared to PDRN, particularly at higher concentrations. In wound-healing assays, L-PDRN demonstrated superior efficacy in promoting cell migration and wound closure, even under inflammatory conditions induced by tumor necrosis factor (TNF-α). Additionally, L-PDRN demonstrated the potential for enhanced immunostimulatory effects under non-inflammatory conditions while maintaining anti-inflammatory properties under lipopolysaccharide (LPS) stimulation. Electrophoretic analysis revealed that L-PDRN consists of smaller DNA fragments (under 100 bp) compared to salmon-derived PDRN (200–800 bp), suggesting greater bioavailability and skin absorption. Mechanistic studies confirmed that L-PDRN activates the focal adhesion kinase (FAK) and protein kinase B (AKT) signaling pathway through the A2A receptor, similar to PDRN, while also engaging alternative pathways for p38 and ERK phosphorylation, highlighting its signaling versatility. This study underscores the potential of L-PDRN as a multifunctional and sustainable alternative to salmon-derived PDRN, offering enhanced bioactivity, scalability, and environmental benefits. The novel approach of utilizing microbial-derived PDRN opens new avenues for therapeutic applications in oxidative stress management, tissue regeneration, and immune modulation, paving the way for a paradigm shift in PDRN sourcing and functionality. |
| format | Article |
| id | doaj-art-c0cfe208ee28486987f276a3f7f27837 |
| institution | Kabale University |
| issn | 1467-3037 1467-3045 |
| language | English |
| publishDate | 2025-01-01 |
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| series | Current Issues in Molecular Biology |
| spelling | doaj-art-c0cfe208ee28486987f276a3f7f278372025-01-24T13:27:30ZengMDPI AGCurrent Issues in Molecular Biology1467-30371467-30452025-01-014714110.3390/cimb47010041First Report on Microbial-Derived Polydeoxyribonucleotide: A Sustainable and Enhanced Alternative to Salmon-Based PolydeoxyribonucleotideDoobyeong Chae0Sae-Woong Oh1Yoon-Seo Choi2Dae-Jung Kang3Chun-Woong Park4Jongsung Lee5Won-Sang Seo6Department of Cosmetics Industry, Graduate School, Chungbuk National University, Cheongju 28160, Republic of KoreaMolecular Dermatology Laboratory, Department of Integrative Biotechnology, College of Biotechnology and Bioengineering, Sungkyunkwan University, Suwon 16419, Republic of KoreaGraduate School-Interdisciplinary Program in Biocosmetics, Sungkyunkwan University, Suwon 16419, Republic of KoreaMNH Bio Co., Ltd., Hwaseong 18469, Republic of KoreaDepartment of Manufacturing Pharmacy, College of Pharmacy, Chungbuk National University, Cheongju 28160, Republic of KoreaMolecular Dermatology Laboratory, Department of Integrative Biotechnology, College of Biotechnology and Bioengineering, Sungkyunkwan University, Suwon 16419, Republic of KoreaCOSLINK, Songpa-gu, Seoul 05819, Republic of KoreaPolydeoxyribonucleotide (PDRN) has emerged as a potent bioactive compound with proven efficacy in wound healing, tissue regeneration, and anti-inflammatory applications and is predominantly derived from salmonid gonads. However, this study presents a groundbreaking advancement by successfully extracting and characterizing PDRN from microbial sources, specifically <i>Lactobacillus rhamnosus</i>, marking the first report to utilize microbial-, biome-, or <i>Lactobacillus</i>-derived PDRN (L-PDRN). The findings demonstrate the enhanced biological properties of L-PDRN over traditional salmon-derived PDRN across several assays. L-PDRN exhibited superior antioxidant activity, with significantly higher SOD-like and DPPH radical scavenging activities compared to PDRN, particularly at higher concentrations. In wound-healing assays, L-PDRN demonstrated superior efficacy in promoting cell migration and wound closure, even under inflammatory conditions induced by tumor necrosis factor (TNF-α). Additionally, L-PDRN demonstrated the potential for enhanced immunostimulatory effects under non-inflammatory conditions while maintaining anti-inflammatory properties under lipopolysaccharide (LPS) stimulation. Electrophoretic analysis revealed that L-PDRN consists of smaller DNA fragments (under 100 bp) compared to salmon-derived PDRN (200–800 bp), suggesting greater bioavailability and skin absorption. Mechanistic studies confirmed that L-PDRN activates the focal adhesion kinase (FAK) and protein kinase B (AKT) signaling pathway through the A2A receptor, similar to PDRN, while also engaging alternative pathways for p38 and ERK phosphorylation, highlighting its signaling versatility. This study underscores the potential of L-PDRN as a multifunctional and sustainable alternative to salmon-derived PDRN, offering enhanced bioactivity, scalability, and environmental benefits. The novel approach of utilizing microbial-derived PDRN opens new avenues for therapeutic applications in oxidative stress management, tissue regeneration, and immune modulation, paving the way for a paradigm shift in PDRN sourcing and functionality.https://www.mdpi.com/1467-3045/47/1/41PDRN (polydeoxyribonucleotide)microbial-derived PDRNprobioticswound healinganti-inflammatory effects |
| spellingShingle | Doobyeong Chae Sae-Woong Oh Yoon-Seo Choi Dae-Jung Kang Chun-Woong Park Jongsung Lee Won-Sang Seo First Report on Microbial-Derived Polydeoxyribonucleotide: A Sustainable and Enhanced Alternative to Salmon-Based Polydeoxyribonucleotide Current Issues in Molecular Biology PDRN (polydeoxyribonucleotide) microbial-derived PDRN probiotics wound healing anti-inflammatory effects |
| title | First Report on Microbial-Derived Polydeoxyribonucleotide: A Sustainable and Enhanced Alternative to Salmon-Based Polydeoxyribonucleotide |
| title_full | First Report on Microbial-Derived Polydeoxyribonucleotide: A Sustainable and Enhanced Alternative to Salmon-Based Polydeoxyribonucleotide |
| title_fullStr | First Report on Microbial-Derived Polydeoxyribonucleotide: A Sustainable and Enhanced Alternative to Salmon-Based Polydeoxyribonucleotide |
| title_full_unstemmed | First Report on Microbial-Derived Polydeoxyribonucleotide: A Sustainable and Enhanced Alternative to Salmon-Based Polydeoxyribonucleotide |
| title_short | First Report on Microbial-Derived Polydeoxyribonucleotide: A Sustainable and Enhanced Alternative to Salmon-Based Polydeoxyribonucleotide |
| title_sort | first report on microbial derived polydeoxyribonucleotide a sustainable and enhanced alternative to salmon based polydeoxyribonucleotide |
| topic | PDRN (polydeoxyribonucleotide) microbial-derived PDRN probiotics wound healing anti-inflammatory effects |
| url | https://www.mdpi.com/1467-3045/47/1/41 |
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