CD133 Antigen as a Potential Marker of Melanoma Stem Cells: In Vitro and In Vivo Studies
Melanoma is the most dangerous type of skin cancer. Cancer stem cells (CSCs) are suspected to be responsible for the cancer recurrence and in the consequence for cancer therapy failure. CD133 is a potential marker for detection of melanoma CSCs. Experiments were performed on the B16-F10 mouse melano...
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| Format: | Article |
| Language: | English |
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Wiley
2020-01-01
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| Series: | Stem Cells International |
| Online Access: | http://dx.doi.org/10.1155/2020/8810476 |
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| author | Tomasz Kloskowski Joanna Jarząbkowska Arkadiusz Jundziłł Daria Balcerczyk Monika Buhl Kamil Szeliski Magdalena Bodnar Andrzej Marszałek Gerard Drewa Tomasz Drewa Marta Pokrywczyńska |
| author_facet | Tomasz Kloskowski Joanna Jarząbkowska Arkadiusz Jundziłł Daria Balcerczyk Monika Buhl Kamil Szeliski Magdalena Bodnar Andrzej Marszałek Gerard Drewa Tomasz Drewa Marta Pokrywczyńska |
| author_sort | Tomasz Kloskowski |
| collection | DOAJ |
| description | Melanoma is the most dangerous type of skin cancer. Cancer stem cells (CSCs) are suspected to be responsible for the cancer recurrence and in the consequence for cancer therapy failure. CD133 is a potential marker for detection of melanoma CSCs. Experiments were performed on the B16-F10 mouse melanoma cell line. CD133+ cells were isolated using an immunomagnetic cell sorting technique. After isolation proliferative and clonogenic potential of CD133+, CD133- and CD133+/- were evaluated. The potential of CD133+ and CD133- cells for tumor induction was conducted on C57BL/6J mouse model. Three different cell quantities (100, 1000, 10000) were tested. Tumor morphology, number of mitoses, and tumor necrosis area were analyzed. Average 0.12% CD133+ cells were isolated. Compared to CD133- and unsorted CD133+/- cells, CD133+ cells were characterized by the higher proliferative and clonogenic potential. These properties were not confirmed in vivo, as both CD133+ and CD133- cells induced tumor growth in mouse model. No statistical differences in mitosis number and tumor necrosis area were observed. Simultaneous detection of CD133 antigen with other markers is necessary for accurate identification of these melanoma cancer stem cells. |
| format | Article |
| id | doaj-art-c0cccdb90b514433951f1ff907c3292a |
| institution | Kabale University |
| issn | 1687-966X 1687-9678 |
| language | English |
| publishDate | 2020-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Stem Cells International |
| spelling | doaj-art-c0cccdb90b514433951f1ff907c3292a2025-02-03T06:46:30ZengWileyStem Cells International1687-966X1687-96782020-01-01202010.1155/2020/88104768810476CD133 Antigen as a Potential Marker of Melanoma Stem Cells: In Vitro and In Vivo StudiesTomasz Kloskowski0Joanna Jarząbkowska1Arkadiusz Jundziłł2Daria Balcerczyk3Monika Buhl4Kamil Szeliski5Magdalena Bodnar6Andrzej Marszałek7Gerard Drewa8Tomasz Drewa9Marta Pokrywczyńska10Chair of Urology and Andrology, Department of Regenerative Medicine, Cell and Tissue Bank, Collegium Medicum, Nicolaus Copernicus University, Sklodowskiej-Curie 9, 85-094 Bydgoszcz, PolandChair of Urology and Andrology, Department of Regenerative Medicine, Cell and Tissue Bank, Collegium Medicum, Nicolaus Copernicus University, Sklodowskiej-Curie 9, 85-094 Bydgoszcz, PolandChair of Urology and Andrology, Department of Regenerative Medicine, Cell and Tissue Bank, Collegium Medicum, Nicolaus Copernicus University, Sklodowskiej-Curie 9, 85-094 Bydgoszcz, PolandChair of Urology and Andrology, Department of Regenerative Medicine, Cell and Tissue Bank, Collegium Medicum, Nicolaus Copernicus University, Sklodowskiej-Curie 9, 85-094 Bydgoszcz, PolandChair of Urology and Andrology, Department of Regenerative Medicine, Cell and Tissue Bank, Collegium Medicum, Nicolaus Copernicus University, Sklodowskiej-Curie 9, 85-094 Bydgoszcz, PolandChair of Urology and Andrology, Department of Regenerative Medicine, Cell and Tissue Bank, Collegium Medicum, Nicolaus Copernicus University, Sklodowskiej-Curie 9, 85-094 Bydgoszcz, PolandDepartment of Clinical Pathomorphology, Collegium Medicum, Nicolaus Copernicus University, Bydgoszcz, PolandDepartment of Clinical Pathology, Poznan University of Medical Sciences and Greater Poland Cancer Center, Poznan, PolandUniversity of Bydgoszcz, Bydgoszcz, PolandChair of Urology and Andrology, Department of Regenerative Medicine, Cell and Tissue Bank, Collegium Medicum, Nicolaus Copernicus University, Sklodowskiej-Curie 9, 85-094 Bydgoszcz, PolandChair of Urology and Andrology, Department of Regenerative Medicine, Cell and Tissue Bank, Collegium Medicum, Nicolaus Copernicus University, Sklodowskiej-Curie 9, 85-094 Bydgoszcz, PolandMelanoma is the most dangerous type of skin cancer. Cancer stem cells (CSCs) are suspected to be responsible for the cancer recurrence and in the consequence for cancer therapy failure. CD133 is a potential marker for detection of melanoma CSCs. Experiments were performed on the B16-F10 mouse melanoma cell line. CD133+ cells were isolated using an immunomagnetic cell sorting technique. After isolation proliferative and clonogenic potential of CD133+, CD133- and CD133+/- were evaluated. The potential of CD133+ and CD133- cells for tumor induction was conducted on C57BL/6J mouse model. Three different cell quantities (100, 1000, 10000) were tested. Tumor morphology, number of mitoses, and tumor necrosis area were analyzed. Average 0.12% CD133+ cells were isolated. Compared to CD133- and unsorted CD133+/- cells, CD133+ cells were characterized by the higher proliferative and clonogenic potential. These properties were not confirmed in vivo, as both CD133+ and CD133- cells induced tumor growth in mouse model. No statistical differences in mitosis number and tumor necrosis area were observed. Simultaneous detection of CD133 antigen with other markers is necessary for accurate identification of these melanoma cancer stem cells.http://dx.doi.org/10.1155/2020/8810476 |
| spellingShingle | Tomasz Kloskowski Joanna Jarząbkowska Arkadiusz Jundziłł Daria Balcerczyk Monika Buhl Kamil Szeliski Magdalena Bodnar Andrzej Marszałek Gerard Drewa Tomasz Drewa Marta Pokrywczyńska CD133 Antigen as a Potential Marker of Melanoma Stem Cells: In Vitro and In Vivo Studies Stem Cells International |
| title | CD133 Antigen as a Potential Marker of Melanoma Stem Cells: In Vitro and In Vivo Studies |
| title_full | CD133 Antigen as a Potential Marker of Melanoma Stem Cells: In Vitro and In Vivo Studies |
| title_fullStr | CD133 Antigen as a Potential Marker of Melanoma Stem Cells: In Vitro and In Vivo Studies |
| title_full_unstemmed | CD133 Antigen as a Potential Marker of Melanoma Stem Cells: In Vitro and In Vivo Studies |
| title_short | CD133 Antigen as a Potential Marker of Melanoma Stem Cells: In Vitro and In Vivo Studies |
| title_sort | cd133 antigen as a potential marker of melanoma stem cells in vitro and in vivo studies |
| url | http://dx.doi.org/10.1155/2020/8810476 |
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