Metabolic enzyme inhibitory abilities, in vivo hypoglycemic ability of palmleaf raspberry fruits extracts and identification of hypoglycemic compounds
The unripe palmleaf raspberry, namely Fupenzi (FPZ), is an important medicinal and edible food. This study aims to evaluate the potential of FPZ extracts prepared with different approaches in attenuating hyperglycemia, gout, Alzheimer's disease, and pigmentation, to obtain the enriching fractio...
Saved in:
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Tsinghua University Press
2023-07-01
|
| Series: | Food Science and Human Wellness |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2213453022002245 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832557600017743872 |
|---|---|
| author | Jun Tan Danshu Wang Yu Lu Yehan Wang Zongcai Tu Tao Yuan Lu Zhang |
| author_facet | Jun Tan Danshu Wang Yu Lu Yehan Wang Zongcai Tu Tao Yuan Lu Zhang |
| author_sort | Jun Tan |
| collection | DOAJ |
| description | The unripe palmleaf raspberry, namely Fupenzi (FPZ), is an important medicinal and edible food. This study aims to evaluate the potential of FPZ extracts prepared with different approaches in attenuating hyperglycemia, gout, Alzheimer's disease, and pigmentation, to obtain the enriching fraction and to identify the major active compounds. Results indicated that FPZ extracts showed weak activity against acetylcholinesterase, considerable ability against tyrosinase and xanthine oxidase, but excellent inhibition on α-glucosidase. Ultrasound-assisted 40 % ethanol extract (40EUS) gave the highest phenolics content, and the best α-glucosidase inhibition (IC50 = 0.08 μg/mL), which is 877-fold higher than that of positive control acarbose. The 40 % ethanol eluting fraction of 40EUS showed the strongest a-glucosidase inhibition with the IC50 value of 37.79 ng/mL, it could also effectively attenuate the fasting blood glucose level and oral glucose tolerance of C57BL/6 mice. Twenty-six compounds were identified from 40 % ethanol fraction by using HPLC-QTOF-MS/MS, hydrolysable tannins (including 11 ellagitannins and 4 gallotannins) were the major compounds, phenolic acids came to the second. Above results could provide important technical supporting for the further application and research of FPZ in health foods and drugs against diabetes. |
| format | Article |
| id | doaj-art-c07b26113acc4655b6da58d18950877a |
| institution | Kabale University |
| issn | 2213-4530 |
| language | English |
| publishDate | 2023-07-01 |
| publisher | Tsinghua University Press |
| record_format | Article |
| series | Food Science and Human Wellness |
| spelling | doaj-art-c07b26113acc4655b6da58d18950877a2025-02-03T04:05:21ZengTsinghua University PressFood Science and Human Wellness2213-45302023-07-0112412321240Metabolic enzyme inhibitory abilities, in vivo hypoglycemic ability of palmleaf raspberry fruits extracts and identification of hypoglycemic compoundsJun Tan0Danshu Wang1Yu Lu2Yehan Wang3Zongcai Tu4Tao Yuan5Lu Zhang6National R&D Center for Freshwater Fish Processing, Engineering Research Center of Freshwater Fish High-Value Utilization of Jiangxi Province, College of Life Science, Jiangxi Normal University, Nanchang, Jiangxi 330022, ChinaNational R&D Center for Freshwater Fish Processing, Engineering Research Center of Freshwater Fish High-Value Utilization of Jiangxi Province, College of Life Science, Jiangxi Normal University, Nanchang, Jiangxi 330022, ChinaNational R&D Center for Freshwater Fish Processing, Engineering Research Center of Freshwater Fish High-Value Utilization of Jiangxi Province, College of Life Science, Jiangxi Normal University, Nanchang, Jiangxi 330022, ChinaNational R&D Center for Freshwater Fish Processing, Engineering Research Center of Freshwater Fish High-Value Utilization of Jiangxi Province, College of Life Science, Jiangxi Normal University, Nanchang, Jiangxi 330022, ChinaNational R&D Center for Freshwater Fish Processing, Engineering Research Center of Freshwater Fish High-Value Utilization of Jiangxi Province, College of Life Science, Jiangxi Normal University, Nanchang, Jiangxi 330022, ChinaCorresponding authors at: College of Life Science, Jiangxi Normal University, Nanchang, Jiangxi 330022, China; National R&D Center for Freshwater Fish Processing, Engineering Research Center of Freshwater Fish High-Value Utilization of Jiangxi Province, College of Life Science, Jiangxi Normal University, Nanchang, Jiangxi 330022, ChinaCorresponding authors at: College of Life Science, Jiangxi Normal University, Nanchang, Jiangxi 330022, China; National R&D Center for Freshwater Fish Processing, Engineering Research Center of Freshwater Fish High-Value Utilization of Jiangxi Province, College of Life Science, Jiangxi Normal University, Nanchang, Jiangxi 330022, ChinaThe unripe palmleaf raspberry, namely Fupenzi (FPZ), is an important medicinal and edible food. This study aims to evaluate the potential of FPZ extracts prepared with different approaches in attenuating hyperglycemia, gout, Alzheimer's disease, and pigmentation, to obtain the enriching fraction and to identify the major active compounds. Results indicated that FPZ extracts showed weak activity against acetylcholinesterase, considerable ability against tyrosinase and xanthine oxidase, but excellent inhibition on α-glucosidase. Ultrasound-assisted 40 % ethanol extract (40EUS) gave the highest phenolics content, and the best α-glucosidase inhibition (IC50 = 0.08 μg/mL), which is 877-fold higher than that of positive control acarbose. The 40 % ethanol eluting fraction of 40EUS showed the strongest a-glucosidase inhibition with the IC50 value of 37.79 ng/mL, it could also effectively attenuate the fasting blood glucose level and oral glucose tolerance of C57BL/6 mice. Twenty-six compounds were identified from 40 % ethanol fraction by using HPLC-QTOF-MS/MS, hydrolysable tannins (including 11 ellagitannins and 4 gallotannins) were the major compounds, phenolic acids came to the second. Above results could provide important technical supporting for the further application and research of FPZ in health foods and drugs against diabetes.http://www.sciencedirect.com/science/article/pii/S2213453022002245Palmleaf raspberryExtraction and identificationEnzyme inhibitionHPLC-QTOF-MS/MSHypoglycemic activity |
| spellingShingle | Jun Tan Danshu Wang Yu Lu Yehan Wang Zongcai Tu Tao Yuan Lu Zhang Metabolic enzyme inhibitory abilities, in vivo hypoglycemic ability of palmleaf raspberry fruits extracts and identification of hypoglycemic compounds Food Science and Human Wellness Palmleaf raspberry Extraction and identification Enzyme inhibition HPLC-QTOF-MS/MS Hypoglycemic activity |
| title | Metabolic enzyme inhibitory abilities, in vivo hypoglycemic ability of palmleaf raspberry fruits extracts and identification of hypoglycemic compounds |
| title_full | Metabolic enzyme inhibitory abilities, in vivo hypoglycemic ability of palmleaf raspberry fruits extracts and identification of hypoglycemic compounds |
| title_fullStr | Metabolic enzyme inhibitory abilities, in vivo hypoglycemic ability of palmleaf raspberry fruits extracts and identification of hypoglycemic compounds |
| title_full_unstemmed | Metabolic enzyme inhibitory abilities, in vivo hypoglycemic ability of palmleaf raspberry fruits extracts and identification of hypoglycemic compounds |
| title_short | Metabolic enzyme inhibitory abilities, in vivo hypoglycemic ability of palmleaf raspberry fruits extracts and identification of hypoglycemic compounds |
| title_sort | metabolic enzyme inhibitory abilities in vivo hypoglycemic ability of palmleaf raspberry fruits extracts and identification of hypoglycemic compounds |
| topic | Palmleaf raspberry Extraction and identification Enzyme inhibition HPLC-QTOF-MS/MS Hypoglycemic activity |
| url | http://www.sciencedirect.com/science/article/pii/S2213453022002245 |
| work_keys_str_mv | AT juntan metabolicenzymeinhibitoryabilitiesinvivohypoglycemicabilityofpalmleafraspberryfruitsextractsandidentificationofhypoglycemiccompounds AT danshuwang metabolicenzymeinhibitoryabilitiesinvivohypoglycemicabilityofpalmleafraspberryfruitsextractsandidentificationofhypoglycemiccompounds AT yulu metabolicenzymeinhibitoryabilitiesinvivohypoglycemicabilityofpalmleafraspberryfruitsextractsandidentificationofhypoglycemiccompounds AT yehanwang metabolicenzymeinhibitoryabilitiesinvivohypoglycemicabilityofpalmleafraspberryfruitsextractsandidentificationofhypoglycemiccompounds AT zongcaitu metabolicenzymeinhibitoryabilitiesinvivohypoglycemicabilityofpalmleafraspberryfruitsextractsandidentificationofhypoglycemiccompounds AT taoyuan metabolicenzymeinhibitoryabilitiesinvivohypoglycemicabilityofpalmleafraspberryfruitsextractsandidentificationofhypoglycemiccompounds AT luzhang metabolicenzymeinhibitoryabilitiesinvivohypoglycemicabilityofpalmleafraspberryfruitsextractsandidentificationofhypoglycemiccompounds |