<i>Vibrio cholerae</i> Gut Colonization of Zebrafish Larvae Induces a Dampened Sensorimotor Response
<b>Background:</b> Cholera is a diarrheal disease prevalent in populations without access to clean water. Cholera is caused by <i>Vibrio cholerae,</i> which colonizes the upper small intestine in humans once ingested. A growing number of studies suggest that the gut microbiom...
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2025-01-01
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| author | Isabella Cubillejo Kevin R. Theis Jonathan Panzer Xixia Luo Shreya Banerjee Ryan Thummel Jeffrey H. Withey |
| author_facet | Isabella Cubillejo Kevin R. Theis Jonathan Panzer Xixia Luo Shreya Banerjee Ryan Thummel Jeffrey H. Withey |
| author_sort | Isabella Cubillejo |
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| description | <b>Background:</b> Cholera is a diarrheal disease prevalent in populations without access to clean water. Cholera is caused by <i>Vibrio cholerae,</i> which colonizes the upper small intestine in humans once ingested. A growing number of studies suggest that the gut microbiome composition modulates animal behavior. Zebrafish are an established cholera model that can maintain a complex, mature gut microbiome during infection. Larval zebrafish, which have immature gut microbiomes, provide the advantage of high-throughput analyses for established behavioral models. <b>Methods:</b> We identified the effects of <i>V. cholerae</i> O1 El Tor C6706 colonization at 5 days post-fertilization (dpf) on larval zebrafish behavior by tracking startle responses at 10 dpf. We also characterized the larval gut microbiome using 16S rRNA sequencing. <i>V. cholerae</i>-infected or uninfected control groups were exposed to either an alternating light/dark stimuli or a single-tap stimulus, and average distance and velocity were tracked. <b>Results</b>: While there was no significant difference in the light/dark trial, we report a significant decrease in distance moved for C6706-colonized larvae during the single-tap trial. <b>Conclusion:</b> This suggests that early <i>V. cholerae</i> colonization of the larval gut microbiome has a dampening effect on sensorimotor function, supporting the idea of a link between the gut microbiome and behavior. |
| format | Article |
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| institution | Kabale University |
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| spelling | doaj-art-c071d55dbbc044b893011dc3e1dc99de2025-01-24T13:24:28ZengMDPI AGBiomedicines2227-90592025-01-0113122610.3390/biomedicines13010226<i>Vibrio cholerae</i> Gut Colonization of Zebrafish Larvae Induces a Dampened Sensorimotor ResponseIsabella Cubillejo0Kevin R. Theis1Jonathan Panzer2Xixia Luo3Shreya Banerjee4Ryan Thummel5Jeffrey H. Withey6Department of Biochemistry, Microbiology, and Immunology, Wayne State University, Detroit, MI 48201, USADepartment of Biochemistry, Microbiology, and Immunology, Wayne State University, Detroit, MI 48201, USADepartment of Biochemistry, Microbiology, and Immunology, Wayne State University, Detroit, MI 48201, USADepartment of Ophthalmology, Visual and Anatomical Sciences, Wayne State University School of Medicine, Detroit, MI 48201, USADepartment of Ophthalmology, Visual and Anatomical Sciences, Wayne State University School of Medicine, Detroit, MI 48201, USADepartment of Ophthalmology, Visual and Anatomical Sciences, Wayne State University School of Medicine, Detroit, MI 48201, USADepartment of Biochemistry, Microbiology, and Immunology, Wayne State University, Detroit, MI 48201, USA<b>Background:</b> Cholera is a diarrheal disease prevalent in populations without access to clean water. Cholera is caused by <i>Vibrio cholerae,</i> which colonizes the upper small intestine in humans once ingested. A growing number of studies suggest that the gut microbiome composition modulates animal behavior. Zebrafish are an established cholera model that can maintain a complex, mature gut microbiome during infection. Larval zebrafish, which have immature gut microbiomes, provide the advantage of high-throughput analyses for established behavioral models. <b>Methods:</b> We identified the effects of <i>V. cholerae</i> O1 El Tor C6706 colonization at 5 days post-fertilization (dpf) on larval zebrafish behavior by tracking startle responses at 10 dpf. We also characterized the larval gut microbiome using 16S rRNA sequencing. <i>V. cholerae</i>-infected or uninfected control groups were exposed to either an alternating light/dark stimuli or a single-tap stimulus, and average distance and velocity were tracked. <b>Results</b>: While there was no significant difference in the light/dark trial, we report a significant decrease in distance moved for C6706-colonized larvae during the single-tap trial. <b>Conclusion:</b> This suggests that early <i>V. cholerae</i> colonization of the larval gut microbiome has a dampening effect on sensorimotor function, supporting the idea of a link between the gut microbiome and behavior.https://www.mdpi.com/2227-9059/13/1/226<i>Vibrio cholerae</i>cholerazebrafish larvaemicrobiomebehavior |
| spellingShingle | Isabella Cubillejo Kevin R. Theis Jonathan Panzer Xixia Luo Shreya Banerjee Ryan Thummel Jeffrey H. Withey <i>Vibrio cholerae</i> Gut Colonization of Zebrafish Larvae Induces a Dampened Sensorimotor Response Biomedicines <i>Vibrio cholerae</i> cholera zebrafish larvae microbiome behavior |
| title | <i>Vibrio cholerae</i> Gut Colonization of Zebrafish Larvae Induces a Dampened Sensorimotor Response |
| title_full | <i>Vibrio cholerae</i> Gut Colonization of Zebrafish Larvae Induces a Dampened Sensorimotor Response |
| title_fullStr | <i>Vibrio cholerae</i> Gut Colonization of Zebrafish Larvae Induces a Dampened Sensorimotor Response |
| title_full_unstemmed | <i>Vibrio cholerae</i> Gut Colonization of Zebrafish Larvae Induces a Dampened Sensorimotor Response |
| title_short | <i>Vibrio cholerae</i> Gut Colonization of Zebrafish Larvae Induces a Dampened Sensorimotor Response |
| title_sort | i vibrio cholerae i gut colonization of zebrafish larvae induces a dampened sensorimotor response |
| topic | <i>Vibrio cholerae</i> cholera zebrafish larvae microbiome behavior |
| url | https://www.mdpi.com/2227-9059/13/1/226 |
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