A Chinese Traditional Therapy for Bleomycin-Induced Pulmonary Fibrosis in Mice
Pulmonary fibrosis is a chronic and fatal disease of lung tissue with high incidence and mortality in the world. The exploration of effective treatment for pulmonary fibrosis remains an urgent challenge. In our study, Qingfei Xieding was investigated as a novel Chinese traditional patent medicine ag...
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| Format: | Article |
| Language: | English |
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Wiley
2018-01-01
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| Series: | Canadian Respiratory Journal |
| Online Access: | http://dx.doi.org/10.1155/2018/8491487 |
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| author | Lifang Sun Minjie Mao Zhisheng Yan Cuiyun Zuo Xiaojie Zhang |
| author_facet | Lifang Sun Minjie Mao Zhisheng Yan Cuiyun Zuo Xiaojie Zhang |
| author_sort | Lifang Sun |
| collection | DOAJ |
| description | Pulmonary fibrosis is a chronic and fatal disease of lung tissue with high incidence and mortality in the world. The exploration of effective treatment for pulmonary fibrosis remains an urgent challenge. In our study, Qingfei Xieding was investigated as a novel Chinese traditional patent medicine against pulmonary fibrosis. A pulmonary fibrosis mouse model was constructed by injecting with bleomycin sulfate. Following Qingfei Xieding administration, lung samples were collected to assess pulmonary phenotype changes by analyzing lung coefficient, wet/dry, and histopathologic section. Levels of nitric oxide (NO), hydroxyproline (HYP), malondialdehyde (MDA), and total antioxidant capacity were measured to evaluate the degree of oxidation. A single-cell gel electrophoresis (SCGE) assay was used to evaluate bleomycin-induced DNA damage. Western blotting and real-time quantitative PCR were performed to determine the abundance of inducible nitric oxide synthase (iNOS), connective tissue growth factor (CTGF), alpha smooth muscle actin (α-SMA), and fibronectin (FN). In the present study, Qingfei Xieding administration significantly attenuated bleomycin-induced pulmonary fibrosis in mice by reducing lung coefficient, wet/dry, NO, HYP, and MDA as well as the expression of iNOS, CTGF, α-SMA, FN, and DNA damage. The results indicated that Qingfei Xieding is effective to resist oxidative damage and histopathologic lesion, serving a protection role on bleomycin-induced pulmonary fibrosis. |
| format | Article |
| id | doaj-art-c042b48e5178402bb04e1a22e8272ff8 |
| institution | Kabale University |
| issn | 1198-2241 1916-7245 |
| language | English |
| publishDate | 2018-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Canadian Respiratory Journal |
| spelling | doaj-art-c042b48e5178402bb04e1a22e8272ff82025-02-03T06:46:04ZengWileyCanadian Respiratory Journal1198-22411916-72452018-01-01201810.1155/2018/84914878491487A Chinese Traditional Therapy for Bleomycin-Induced Pulmonary Fibrosis in MiceLifang Sun0Minjie Mao1Zhisheng Yan2Cuiyun Zuo3Xiaojie Zhang4Department of Tuberculosis, Hangzhou Red Cross Hospital, Hangzhou 310003, ChinaDepartment of Tuberculosis, Hangzhou Red Cross Hospital, Hangzhou 310003, ChinaDepartment of Critical Care Medicine, Pingdu People’s Hospital, Qingdao 266700, ChinaDepartment of Respiratory Disease, Third Hospital of Xiamen, Xiamen 361100, Fujian, ChinaDepartment of Emergency Medicine, Hangzhou Red Cross Hospital, Hangzhou 310003, ChinaPulmonary fibrosis is a chronic and fatal disease of lung tissue with high incidence and mortality in the world. The exploration of effective treatment for pulmonary fibrosis remains an urgent challenge. In our study, Qingfei Xieding was investigated as a novel Chinese traditional patent medicine against pulmonary fibrosis. A pulmonary fibrosis mouse model was constructed by injecting with bleomycin sulfate. Following Qingfei Xieding administration, lung samples were collected to assess pulmonary phenotype changes by analyzing lung coefficient, wet/dry, and histopathologic section. Levels of nitric oxide (NO), hydroxyproline (HYP), malondialdehyde (MDA), and total antioxidant capacity were measured to evaluate the degree of oxidation. A single-cell gel electrophoresis (SCGE) assay was used to evaluate bleomycin-induced DNA damage. Western blotting and real-time quantitative PCR were performed to determine the abundance of inducible nitric oxide synthase (iNOS), connective tissue growth factor (CTGF), alpha smooth muscle actin (α-SMA), and fibronectin (FN). In the present study, Qingfei Xieding administration significantly attenuated bleomycin-induced pulmonary fibrosis in mice by reducing lung coefficient, wet/dry, NO, HYP, and MDA as well as the expression of iNOS, CTGF, α-SMA, FN, and DNA damage. The results indicated that Qingfei Xieding is effective to resist oxidative damage and histopathologic lesion, serving a protection role on bleomycin-induced pulmonary fibrosis.http://dx.doi.org/10.1155/2018/8491487 |
| spellingShingle | Lifang Sun Minjie Mao Zhisheng Yan Cuiyun Zuo Xiaojie Zhang A Chinese Traditional Therapy for Bleomycin-Induced Pulmonary Fibrosis in Mice Canadian Respiratory Journal |
| title | A Chinese Traditional Therapy for Bleomycin-Induced Pulmonary Fibrosis in Mice |
| title_full | A Chinese Traditional Therapy for Bleomycin-Induced Pulmonary Fibrosis in Mice |
| title_fullStr | A Chinese Traditional Therapy for Bleomycin-Induced Pulmonary Fibrosis in Mice |
| title_full_unstemmed | A Chinese Traditional Therapy for Bleomycin-Induced Pulmonary Fibrosis in Mice |
| title_short | A Chinese Traditional Therapy for Bleomycin-Induced Pulmonary Fibrosis in Mice |
| title_sort | chinese traditional therapy for bleomycin induced pulmonary fibrosis in mice |
| url | http://dx.doi.org/10.1155/2018/8491487 |
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