Effect of selected catechins on doxorubicin antiproliferative efficacy and hepatotoxicity in vitro
Catechins may influence both desirable and undesirable effects of many drugs. In this study, the in vitro effect of (+)-catechin, (-)-epicatechin, (-)-epigallocatechin, (-)-epicatechin gallate, and (-)-epigallocatechin gallate (EGCG) on the efficacy of anticancer drug doxorubicin (DOX) was studied i...
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Sciendo
2014-06-01
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| Series: | Acta Pharmaceutica |
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| author | Rudolfová Petra Hanušová Veronika Skálová Lenka Bártíková Hana Matoušková Petra Boušová Iva |
| author_facet | Rudolfová Petra Hanušová Veronika Skálová Lenka Bártíková Hana Matoušková Petra Boušová Iva |
| author_sort | Rudolfová Petra |
| collection | DOAJ |
| description | Catechins may influence both desirable and undesirable effects of many drugs. In this study, the in vitro effect of (+)-catechin, (-)-epicatechin, (-)-epigallocatechin, (-)-epicatechin gallate, and (-)-epigallocatechin gallate (EGCG) on the efficacy of anticancer drug doxorubicin (DOX) was studied in HCT-8 cancer cells. Rat hepatocytes were used to study the influence of EGCG on DOX hepatotoxicity. Cell proliferation and viability were studied by 3-[4,5-dimethylthiazol- 2-yl]-2,5-diphenyl tetrazolium bromide and neutral red uptake test assays. Formation of reactive oxygen species (ROS) was determined using the dichlorofluorescein assay. All of the studied catechins (1-25 μmol L-1) had no effect on the proliferation of intestinal cancer cells and did not affect the antiproliferative effect of DOX (1-8 μmol L-1) in these cells. Moreover, EGCG at 25 μmol L-1 increased the viability of isolated hepatocytes and significantly protected these cells against DOX-induced toxicity and ROS production. Consumption of EGCG during DOX therapy seems to be safe and beneficial, since EGCG does not decrease DOX anticancer efficacy and could ameliorate DOX hepatotoxicity |
| format | Article |
| id | doaj-art-c0330aaba409440b97fe2d071fae53bb |
| institution | Kabale University |
| issn | 1330-0075 1846-9558 |
| language | English |
| publishDate | 2014-06-01 |
| publisher | Sciendo |
| record_format | Article |
| series | Acta Pharmaceutica |
| spelling | doaj-art-c0330aaba409440b97fe2d071fae53bb2025-02-02T14:17:10ZengSciendoActa Pharmaceutica1330-00751846-95582014-06-0164219920910.2478/acph-2014-0018acph-2014-0018Effect of selected catechins on doxorubicin antiproliferative efficacy and hepatotoxicity in vitroRudolfová Petra0Hanušová Veronika1Skálová Lenka2Bártíková Hana3Matoušková Petra4Boušová Iva5Department of Biochemical Sciences Charles University in Prague, Faculty of Pharmacy in Hradec Králové CZ-500 05 Hradec Králové Czech RepublicDepartment of Biochemical Sciences Charles University in Prague, Faculty of Pharmacy in Hradec Králové CZ-500 05 Hradec Králové Czech RepublicDepartment of Biochemical Sciences Charles University in Prague, Faculty of Pharmacy in Hradec Králové CZ-500 05 Hradec Králové Czech RepublicDepartment of Biochemical Sciences Charles University in Prague, Faculty of Pharmacy in Hradec Králové CZ-500 05 Hradec Králové Czech RepublicDepartment of Biochemical Sciences Charles University in Prague, Faculty of Pharmacy in Hradec Králové CZ-500 05 Hradec Králové Czech RepublicDepartment of Biochemical Sciences Charles University in Prague, Faculty of Pharmacy in Hradec Králové CZ-500 05 Hradec Králové Czech RepublicCatechins may influence both desirable and undesirable effects of many drugs. In this study, the in vitro effect of (+)-catechin, (-)-epicatechin, (-)-epigallocatechin, (-)-epicatechin gallate, and (-)-epigallocatechin gallate (EGCG) on the efficacy of anticancer drug doxorubicin (DOX) was studied in HCT-8 cancer cells. Rat hepatocytes were used to study the influence of EGCG on DOX hepatotoxicity. Cell proliferation and viability were studied by 3-[4,5-dimethylthiazol- 2-yl]-2,5-diphenyl tetrazolium bromide and neutral red uptake test assays. Formation of reactive oxygen species (ROS) was determined using the dichlorofluorescein assay. All of the studied catechins (1-25 μmol L-1) had no effect on the proliferation of intestinal cancer cells and did not affect the antiproliferative effect of DOX (1-8 μmol L-1) in these cells. Moreover, EGCG at 25 μmol L-1 increased the viability of isolated hepatocytes and significantly protected these cells against DOX-induced toxicity and ROS production. Consumption of EGCG during DOX therapy seems to be safe and beneficial, since EGCG does not decrease DOX anticancer efficacy and could ameliorate DOX hepatotoxicityhttp://www.degruyter.com/view/j/acph.2014.64.issue-2/acph-2014-0018/acph-2014-0018.xml?format=INTepigallocatechin gallatechemopreventionHCT- -8 cellshepatocytesdoxorubicin |
| spellingShingle | Rudolfová Petra Hanušová Veronika Skálová Lenka Bártíková Hana Matoušková Petra Boušová Iva Effect of selected catechins on doxorubicin antiproliferative efficacy and hepatotoxicity in vitro Acta Pharmaceutica epigallocatechin gallate chemoprevention HCT- -8 cells hepatocytes doxorubicin |
| title | Effect of selected catechins on doxorubicin antiproliferative efficacy and hepatotoxicity in vitro |
| title_full | Effect of selected catechins on doxorubicin antiproliferative efficacy and hepatotoxicity in vitro |
| title_fullStr | Effect of selected catechins on doxorubicin antiproliferative efficacy and hepatotoxicity in vitro |
| title_full_unstemmed | Effect of selected catechins on doxorubicin antiproliferative efficacy and hepatotoxicity in vitro |
| title_short | Effect of selected catechins on doxorubicin antiproliferative efficacy and hepatotoxicity in vitro |
| title_sort | effect of selected catechins on doxorubicin antiproliferative efficacy and hepatotoxicity in vitro |
| topic | epigallocatechin gallate chemoprevention HCT- -8 cells hepatocytes doxorubicin |
| url | http://www.degruyter.com/view/j/acph.2014.64.issue-2/acph-2014-0018/acph-2014-0018.xml?format=INT |
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