NIR‐II emissive biohybrid nanovesicles as mild‐temperature photothermal antibiofilm agents against acute bacterial skin and skin‐structure infections
Abstract The emergence of antibiotic‐resistant bacteria poses a significant challenge to the prompt and appropriate treatment of pathogenic bacteria infections, such as acute bacterial skin and skin‐structure infections (ABSSSI), especially in the presence of biofilms. Bacterial biofilms are natural...
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| Format: | Article |
| Language: | English |
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Wiley-VCH
2025-01-01
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| Series: | Interdisciplinary Medicine |
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| Online Access: | https://doi.org/10.1002/INMD.20240053 |
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| author | Ji Wang Zhihao Wu Xiaoxi Ma Zhihui Huang Haorong Dong Jinxin Zhang Xiaoming Liu Pengfei Zhang Shuhuai Yao |
| author_facet | Ji Wang Zhihao Wu Xiaoxi Ma Zhihui Huang Haorong Dong Jinxin Zhang Xiaoming Liu Pengfei Zhang Shuhuai Yao |
| author_sort | Ji Wang |
| collection | DOAJ |
| description | Abstract The emergence of antibiotic‐resistant bacteria poses a significant challenge to the prompt and appropriate treatment of pathogenic bacteria infections, such as acute bacterial skin and skin‐structure infections (ABSSSI), especially in the presence of biofilms. Bacterial biofilms are naturally resistant to antibiotics and the human immune system, making biofilm‐based infections extremely difficult to treat. Therefore, developing new antibacterial therapies targeting biofilms is crucial. Aggregation‐induced emission luminogens with fluorescence in the second near‐infrared window (NIR‐II AIEgens), which can be activated by a near‐infrared laser to generate heat, offer an effective and precise photothermal therapy (PTT) approach for treating deep‐tissue bacterial infections. However, the presence of biofilms impedes the entry of photosensitizers into the infected area, requiring higher drug doses and increasing the risk of PTT. Herein, we developed a biocompatible AIEgen‐based biohybrid nano formulation that incorporates the BPBBT (NIR‐II AIEgen) and antibiofilm α‐amylase into a red blood cell (RBC) membrane‐derived nanovesicle carrier for a PTT/biofilm degradation combination therapy. The synergistic effect of this new formulation enhances both the photothermal capability of BPBBT and the biofilm degradation compared to traditional individual treatments. The new combination therapy demonstrated significant improvement in treating severe Staphylococcus aureus infections caused by biofilms in vitro and in vivo, presenting a promising alternative to traditional antibiotic therapy. |
| format | Article |
| id | doaj-art-c02dee61e338420fa4c0a99ebb0c923e |
| institution | Kabale University |
| issn | 2832-6245 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Wiley-VCH |
| record_format | Article |
| series | Interdisciplinary Medicine |
| spelling | doaj-art-c02dee61e338420fa4c0a99ebb0c923e2025-01-25T17:57:32ZengWiley-VCHInterdisciplinary Medicine2832-62452025-01-0131n/an/a10.1002/INMD.20240053NIR‐II emissive biohybrid nanovesicles as mild‐temperature photothermal antibiofilm agents against acute bacterial skin and skin‐structure infectionsJi Wang0Zhihao Wu1Xiaoxi Ma2Zhihui Huang3Haorong Dong4Jinxin Zhang5Xiaoming Liu6Pengfei Zhang7Shuhuai Yao8Department of Mechanical and Aerospace Engineering The Hong Kong University of Science and Technology Hong Kong ChinaFunction Hub The Hong Kong University of Science and Technology (Guangzhou) Guangzhou Guangdong ChinaGuangdong Key Laboratory of Nanomedicine CAS‐HK Joint Lab of Biomaterials CAS Key Laboratory of Biomedical Imaging Science and System Shenzhen Engineering Laboratory of Nanomedicine and Nanoformulations CAS Key Lab for Health Informatics Shenzhen Institutes of Advanced Technology Chinese Academy of Sciences Shenzhen ChinaGuangdong Key Laboratory of Nanomedicine CAS‐HK Joint Lab of Biomaterials CAS Key Laboratory of Biomedical Imaging Science and System Shenzhen Engineering Laboratory of Nanomedicine and Nanoformulations CAS Key Lab for Health Informatics Shenzhen Institutes of Advanced Technology Chinese Academy of Sciences Shenzhen ChinaIndividualized Interdisciplinary Program The Hong Kong University of Science and Technology Hong Kong ChinaGuangdong Key Laboratory of Nanomedicine CAS‐HK Joint Lab of Biomaterials CAS Key Laboratory of Biomedical Imaging Science and System Shenzhen Engineering Laboratory of Nanomedicine and Nanoformulations CAS Key Lab for Health Informatics Shenzhen Institutes of Advanced Technology Chinese Academy of Sciences Shenzhen ChinaDepartment of Dermatology Southern University of Science and Technology Hospital Shenzhen ChinaGuangdong Key Laboratory of Nanomedicine CAS‐HK Joint Lab of Biomaterials CAS Key Laboratory of Biomedical Imaging Science and System Shenzhen Engineering Laboratory of Nanomedicine and Nanoformulations CAS Key Lab for Health Informatics Shenzhen Institutes of Advanced Technology Chinese Academy of Sciences Shenzhen ChinaDepartment of Mechanical and Aerospace Engineering The Hong Kong University of Science and Technology Hong Kong ChinaAbstract The emergence of antibiotic‐resistant bacteria poses a significant challenge to the prompt and appropriate treatment of pathogenic bacteria infections, such as acute bacterial skin and skin‐structure infections (ABSSSI), especially in the presence of biofilms. Bacterial biofilms are naturally resistant to antibiotics and the human immune system, making biofilm‐based infections extremely difficult to treat. Therefore, developing new antibacterial therapies targeting biofilms is crucial. Aggregation‐induced emission luminogens with fluorescence in the second near‐infrared window (NIR‐II AIEgens), which can be activated by a near‐infrared laser to generate heat, offer an effective and precise photothermal therapy (PTT) approach for treating deep‐tissue bacterial infections. However, the presence of biofilms impedes the entry of photosensitizers into the infected area, requiring higher drug doses and increasing the risk of PTT. Herein, we developed a biocompatible AIEgen‐based biohybrid nano formulation that incorporates the BPBBT (NIR‐II AIEgen) and antibiofilm α‐amylase into a red blood cell (RBC) membrane‐derived nanovesicle carrier for a PTT/biofilm degradation combination therapy. The synergistic effect of this new formulation enhances both the photothermal capability of BPBBT and the biofilm degradation compared to traditional individual treatments. The new combination therapy demonstrated significant improvement in treating severe Staphylococcus aureus infections caused by biofilms in vitro and in vivo, presenting a promising alternative to traditional antibiotic therapy.https://doi.org/10.1002/INMD.20240053aggregation‐induced emissionanti‐biofilm materialsbiofilmnanovesiclephotothermal therapy |
| spellingShingle | Ji Wang Zhihao Wu Xiaoxi Ma Zhihui Huang Haorong Dong Jinxin Zhang Xiaoming Liu Pengfei Zhang Shuhuai Yao NIR‐II emissive biohybrid nanovesicles as mild‐temperature photothermal antibiofilm agents against acute bacterial skin and skin‐structure infections Interdisciplinary Medicine aggregation‐induced emission anti‐biofilm materials biofilm nanovesicle photothermal therapy |
| title | NIR‐II emissive biohybrid nanovesicles as mild‐temperature photothermal antibiofilm agents against acute bacterial skin and skin‐structure infections |
| title_full | NIR‐II emissive biohybrid nanovesicles as mild‐temperature photothermal antibiofilm agents against acute bacterial skin and skin‐structure infections |
| title_fullStr | NIR‐II emissive biohybrid nanovesicles as mild‐temperature photothermal antibiofilm agents against acute bacterial skin and skin‐structure infections |
| title_full_unstemmed | NIR‐II emissive biohybrid nanovesicles as mild‐temperature photothermal antibiofilm agents against acute bacterial skin and skin‐structure infections |
| title_short | NIR‐II emissive biohybrid nanovesicles as mild‐temperature photothermal antibiofilm agents against acute bacterial skin and skin‐structure infections |
| title_sort | nir ii emissive biohybrid nanovesicles as mild temperature photothermal antibiofilm agents against acute bacterial skin and skin structure infections |
| topic | aggregation‐induced emission anti‐biofilm materials biofilm nanovesicle photothermal therapy |
| url | https://doi.org/10.1002/INMD.20240053 |
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