Change in the Microviscosity of Erythrocyte Membranes and Proteins in Blood Plasma after Graphene Oxide Addition: The ESR Spectroscopy Study
The change in the microviscosity of erythrocyte membranes and the proteins in blood plasma after graphene oxide addition is studied by the ESR spectroscopy exploiting two spin probes with different lipophilic components in the structures. Experiments with charged spin probe 2 embedded into the eryth...
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| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2019-01-01
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| Series: | Journal of Spectroscopy |
| Online Access: | http://dx.doi.org/10.1155/2019/8083207 |
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| _version_ | 1832551900585656320 |
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| author | V. A. Karachevtsev N. T. Kartel L. V. Ivanov A. N. Lyapunov O. A. Nardid Ya. O. Cherkashina A. Yu. Ivanov |
| author_facet | V. A. Karachevtsev N. T. Kartel L. V. Ivanov A. N. Lyapunov O. A. Nardid Ya. O. Cherkashina A. Yu. Ivanov |
| author_sort | V. A. Karachevtsev |
| collection | DOAJ |
| description | The change in the microviscosity of erythrocyte membranes and the proteins in blood plasma after graphene oxide addition is studied by the ESR spectroscopy exploiting two spin probes with different lipophilic components in the structures. Experiments with charged spin probe 2 embedded into the erythrocyte membrane showed that the introduction of graphene oxide in small concentrations (∼70 μg/ml) into a suspension of erythrocytes did not lead to significant changes in the microviscosity of their membranes. Correlation times of hydrophobic spin probe 1 adsorbed to hydrophobic pockets of plasma proteins demonstrate a gradual slowdown at the graphene oxide injection into blood plasma that indicates a small deformation of the hydrophobic cavity of protein at the adsorption. However, this protein binding with graphene oxide does not cause the displacement of the spin probe from their hydrophobic cavities, which is evidence about small changes in the protein secondary structure. The obtained results indicate the insignificant cytotoxicity effect of small concentrations of graphene oxide for erythrocytes and blood plasma. |
| format | Article |
| id | doaj-art-c028f586f47d434dad60d9e06dd20fd2 |
| institution | Kabale University |
| issn | 2314-4920 2314-4939 |
| language | English |
| publishDate | 2019-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Spectroscopy |
| spelling | doaj-art-c028f586f47d434dad60d9e06dd20fd22025-02-03T06:00:10ZengWileyJournal of Spectroscopy2314-49202314-49392019-01-01201910.1155/2019/80832078083207Change in the Microviscosity of Erythrocyte Membranes and Proteins in Blood Plasma after Graphene Oxide Addition: The ESR Spectroscopy StudyV. A. Karachevtsev0N. T. Kartel1L. V. Ivanov2A. N. Lyapunov3O. A. Nardid4Ya. O. Cherkashina5A. Yu. Ivanov6B. Verkin Institute for Low Temperature Physics and Engineering, National Academy of Sciences of Ukraine, Kharkiv, UkraineO. Chuiko Institute of Surface Chemistry, National Academy of Sciences of Ukraine, Kiev, UkraineO. Chuiko Institute of Surface Chemistry, National Academy of Sciences of Ukraine, Kiev, UkraineInstitute for Single Crystals, National Academy of Sciences of Ukraine, Kharkiv, UkraineInstitute for Problems of Cryobiology and Cryomedicine, National Academy of Sciences of Ukraine, Kharkiv, UkraineInstitute for Problems of Cryobiology and Cryomedicine, National Academy of Sciences of Ukraine, Kharkiv, UkraineB. Verkin Institute for Low Temperature Physics and Engineering, National Academy of Sciences of Ukraine, Kharkiv, UkraineThe change in the microviscosity of erythrocyte membranes and the proteins in blood plasma after graphene oxide addition is studied by the ESR spectroscopy exploiting two spin probes with different lipophilic components in the structures. Experiments with charged spin probe 2 embedded into the erythrocyte membrane showed that the introduction of graphene oxide in small concentrations (∼70 μg/ml) into a suspension of erythrocytes did not lead to significant changes in the microviscosity of their membranes. Correlation times of hydrophobic spin probe 1 adsorbed to hydrophobic pockets of plasma proteins demonstrate a gradual slowdown at the graphene oxide injection into blood plasma that indicates a small deformation of the hydrophobic cavity of protein at the adsorption. However, this protein binding with graphene oxide does not cause the displacement of the spin probe from their hydrophobic cavities, which is evidence about small changes in the protein secondary structure. The obtained results indicate the insignificant cytotoxicity effect of small concentrations of graphene oxide for erythrocytes and blood plasma.http://dx.doi.org/10.1155/2019/8083207 |
| spellingShingle | V. A. Karachevtsev N. T. Kartel L. V. Ivanov A. N. Lyapunov O. A. Nardid Ya. O. Cherkashina A. Yu. Ivanov Change in the Microviscosity of Erythrocyte Membranes and Proteins in Blood Plasma after Graphene Oxide Addition: The ESR Spectroscopy Study Journal of Spectroscopy |
| title | Change in the Microviscosity of Erythrocyte Membranes and Proteins in Blood Plasma after Graphene Oxide Addition: The ESR Spectroscopy Study |
| title_full | Change in the Microviscosity of Erythrocyte Membranes and Proteins in Blood Plasma after Graphene Oxide Addition: The ESR Spectroscopy Study |
| title_fullStr | Change in the Microviscosity of Erythrocyte Membranes and Proteins in Blood Plasma after Graphene Oxide Addition: The ESR Spectroscopy Study |
| title_full_unstemmed | Change in the Microviscosity of Erythrocyte Membranes and Proteins in Blood Plasma after Graphene Oxide Addition: The ESR Spectroscopy Study |
| title_short | Change in the Microviscosity of Erythrocyte Membranes and Proteins in Blood Plasma after Graphene Oxide Addition: The ESR Spectroscopy Study |
| title_sort | change in the microviscosity of erythrocyte membranes and proteins in blood plasma after graphene oxide addition the esr spectroscopy study |
| url | http://dx.doi.org/10.1155/2019/8083207 |
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