In Vitro Cadmium-Induced Alterations in Growth and Oxidative Metabolism of Upland Cotton (Gossypium hirsutum L.)
Cadmium (Cd) is a toxic pollutant, which cause both dose- and time-dependent physiological and biochemical alterations in plants. The present in vitro study was undertaken to explore Cd-induced physiological and biochemical changes in cotton callus culture at 0, 550, 700, 850, and 1000 μM Cd for fou...
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Wiley
2014-01-01
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| Series: | The Scientific World Journal |
| Online Access: | http://dx.doi.org/10.1155/2014/309409 |
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| author | M. K. Daud Lei Mei Ullah Najeeb Muhammad Azim Khan Farah Deeba Irum Raza Aliya Batool S. J. Zhu |
| author_facet | M. K. Daud Lei Mei Ullah Najeeb Muhammad Azim Khan Farah Deeba Irum Raza Aliya Batool S. J. Zhu |
| author_sort | M. K. Daud |
| collection | DOAJ |
| description | Cadmium (Cd) is a toxic pollutant, which cause both dose- and time-dependent physiological and biochemical alterations in plants. The present in vitro study was undertaken to explore Cd-induced physiological and biochemical changes in cotton callus culture at 0, 550, 700, 850, and 1000 μM Cd for four different stress periods (7, 14, 21, and 28 days). At 1000 μM Cd, mean growth values were lower than their respective control. The cell protein contents decreased only after 7-day and 14-day stress treatment. At 550 μM Cd, malondialdehyde (MDA) contents decreased after various stress periods except 21-day period. Superoxide dismutase (SOD) activity at 1000 μM Cd improved relative to its respective controls in the first three stress regimes. Almost a decreasing trend in the hydrogen peroxide (H2O2) and peroxidase (POD) activities at all Cd levels after different stress periods was noticed. Ascorbate peroxidase (APX) activity descended over its relevant controls in the first three stress regimes except at 700 μM Cd after 14- and 21-day stress duration. Moreover, catalase (CAT) mean values significantly increased as a whole. From this experiment, it can be concluded that lipid peroxidation as well as reactive oxygen species (ROS) production was relatively higher as has been revealed by higher MDA contents and greater SOD, CAT activities. |
| format | Article |
| id | doaj-art-c011627e15a64d6fbad8e0683b62dcae |
| institution | Kabale University |
| issn | 2356-6140 1537-744X |
| language | English |
| publishDate | 2014-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | The Scientific World Journal |
| spelling | doaj-art-c011627e15a64d6fbad8e0683b62dcae2025-02-03T05:59:24ZengWileyThe Scientific World Journal2356-61401537-744X2014-01-01201410.1155/2014/309409309409In Vitro Cadmium-Induced Alterations in Growth and Oxidative Metabolism of Upland Cotton (Gossypium hirsutum L.)M. K. Daud0Lei Mei1Ullah Najeeb2Muhammad Azim Khan3Farah Deeba4Irum Raza5Aliya Batool6S. J. Zhu7Department of Agronomy, College of Agriculture and Biotechnology, Zhejiang University, Zijingang Campus, Hangzhou 310058, ChinaDepartment of Agronomy, College of Agriculture and Biotechnology, Zhejiang University, Zijingang Campus, Hangzhou 310058, ChinaDepartment of Agronomy, College of Agriculture and Biotechnology, Zhejiang University, Zijingang Campus, Hangzhou 310058, ChinaDepartment of Weed Science, The University of Agriculture, Peshawar, PakistanDepartment of Biotechnology and Genetic Engineering, Kohat University of Science and Technology, Kohat 26000, PakistanDepartment of Biotechnology and Genetic Engineering, Kohat University of Science and Technology, Kohat 26000, PakistanDepartment of Biotechnology and Genetic Engineering, Kohat University of Science and Technology, Kohat 26000, PakistanDepartment of Agronomy, College of Agriculture and Biotechnology, Zhejiang University, Zijingang Campus, Hangzhou 310058, ChinaCadmium (Cd) is a toxic pollutant, which cause both dose- and time-dependent physiological and biochemical alterations in plants. The present in vitro study was undertaken to explore Cd-induced physiological and biochemical changes in cotton callus culture at 0, 550, 700, 850, and 1000 μM Cd for four different stress periods (7, 14, 21, and 28 days). At 1000 μM Cd, mean growth values were lower than their respective control. The cell protein contents decreased only after 7-day and 14-day stress treatment. At 550 μM Cd, malondialdehyde (MDA) contents decreased after various stress periods except 21-day period. Superoxide dismutase (SOD) activity at 1000 μM Cd improved relative to its respective controls in the first three stress regimes. Almost a decreasing trend in the hydrogen peroxide (H2O2) and peroxidase (POD) activities at all Cd levels after different stress periods was noticed. Ascorbate peroxidase (APX) activity descended over its relevant controls in the first three stress regimes except at 700 μM Cd after 14- and 21-day stress duration. Moreover, catalase (CAT) mean values significantly increased as a whole. From this experiment, it can be concluded that lipid peroxidation as well as reactive oxygen species (ROS) production was relatively higher as has been revealed by higher MDA contents and greater SOD, CAT activities.http://dx.doi.org/10.1155/2014/309409 |
| spellingShingle | M. K. Daud Lei Mei Ullah Najeeb Muhammad Azim Khan Farah Deeba Irum Raza Aliya Batool S. J. Zhu In Vitro Cadmium-Induced Alterations in Growth and Oxidative Metabolism of Upland Cotton (Gossypium hirsutum L.) The Scientific World Journal |
| title | In Vitro Cadmium-Induced Alterations in Growth and Oxidative Metabolism of Upland Cotton (Gossypium hirsutum L.) |
| title_full | In Vitro Cadmium-Induced Alterations in Growth and Oxidative Metabolism of Upland Cotton (Gossypium hirsutum L.) |
| title_fullStr | In Vitro Cadmium-Induced Alterations in Growth and Oxidative Metabolism of Upland Cotton (Gossypium hirsutum L.) |
| title_full_unstemmed | In Vitro Cadmium-Induced Alterations in Growth and Oxidative Metabolism of Upland Cotton (Gossypium hirsutum L.) |
| title_short | In Vitro Cadmium-Induced Alterations in Growth and Oxidative Metabolism of Upland Cotton (Gossypium hirsutum L.) |
| title_sort | in vitro cadmium induced alterations in growth and oxidative metabolism of upland cotton gossypium hirsutum l |
| url | http://dx.doi.org/10.1155/2014/309409 |
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