Altered expression of miRNA profile in peripheral blood mononuclear cells following the third dose of inactivated COVID-19 vaccine
COVID-19 vaccination is the most effective strategy for preventing severe disease and death. Inactivated vaccines are the most accessible type of COVID-19 vaccines in developing countries. Several studies, including work from our group, have demonstrated that the third dose (booster vaccination) of...
Saved in:
| Main Authors: | , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
PeerJ Inc.
2025-01-01
|
| Series: | PeerJ |
| Subjects: | |
| Online Access: | https://peerj.com/articles/18856.pdf |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832587244957859840 |
|---|---|
| author | Guanguan Qiu Ruoyang Zhang Huifeng Qian Ruoqiong Huang Jie Xia Ruoxi Zang Zhenkai Le Qiang Shu Jianguo Xu Guoping Zheng Jiangmei Wang |
| author_facet | Guanguan Qiu Ruoyang Zhang Huifeng Qian Ruoqiong Huang Jie Xia Ruoxi Zang Zhenkai Le Qiang Shu Jianguo Xu Guoping Zheng Jiangmei Wang |
| author_sort | Guanguan Qiu |
| collection | DOAJ |
| description | COVID-19 vaccination is the most effective strategy for preventing severe disease and death. Inactivated vaccines are the most accessible type of COVID-19 vaccines in developing countries. Several studies, including work from our group, have demonstrated that the third dose (booster vaccination) of inactivated COVID-19 vaccine induces robust humoral and cellular immune responses. The present study aimed to examine miRNA expression profile in participants who received a homologous third dose of the CoronaVac vaccine. Samples of peripheral blood mononuclear cells (PBMCs) were collected from healthcare volunteers both before and 1–2 weeks after the booster dose. miRNA microarray analysis in a discovery cohort of six volunteers identified 67 miRNAs with differential expression. Subsequently, the expression of six miRNAs related to immune responses was examined in a validation cohort of 31 participants via qRT-PCR. Our results validated the differential expression of miR-25-5p, miR-34c-3p, and miR-206 post-booster, with a significant correlation to the receptor binding domain (RBD)-specific antibody. Bioinformatic analysis suggested that miR-25-5p, miR-34c-3p, and miR-206 may target multiple pathways involved in immune regulation and inflammation. Therefore, our study highlights miR-25-5p, miR-34c-3p, and miR-206 in PBMCs as promising biomarkers for assessing the immune response induced by the booster dose of the CoronaVac vaccine. |
| format | Article |
| id | doaj-art-bfe4b759f9d349758fc79eaea7a9a27b |
| institution | Kabale University |
| issn | 2167-8359 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | PeerJ Inc. |
| record_format | Article |
| series | PeerJ |
| spelling | doaj-art-bfe4b759f9d349758fc79eaea7a9a27b2025-01-24T15:05:06ZengPeerJ Inc.PeerJ2167-83592025-01-0113e1885610.7717/peerj.18856Altered expression of miRNA profile in peripheral blood mononuclear cells following the third dose of inactivated COVID-19 vaccineGuanguan Qiu0Ruoyang Zhang1Huifeng Qian2Ruoqiong Huang3Jie Xia4Ruoxi Zang5Zhenkai Le6Qiang Shu7Jianguo Xu8Guoping Zheng9Jiangmei Wang10Shaoxing Second Hospital, Shaoxing, Zhejiang, ChinaChildren’s Hospital of Zhejiang University School of Medicine, Hangzhou, ChinaShaoxing Second Hospital, Shaoxing, Zhejiang, ChinaChildren’s Hospital of Zhejiang University School of Medicine, Hangzhou, ChinaChildren’s Hospital of Zhejiang University School of Medicine, Hangzhou, ChinaChildren’s Hospital of Zhejiang University School of Medicine, Hangzhou, ChinaChildren’s Hospital of Zhejiang University School of Medicine, Hangzhou, ChinaChildren’s Hospital of Zhejiang University School of Medicine, Hangzhou, ChinaShaoxing Second Hospital, Shaoxing, Zhejiang, ChinaShaoxing Second Hospital, Shaoxing, Zhejiang, ChinaChildren’s Hospital of Zhejiang University School of Medicine, Hangzhou, ChinaCOVID-19 vaccination is the most effective strategy for preventing severe disease and death. Inactivated vaccines are the most accessible type of COVID-19 vaccines in developing countries. Several studies, including work from our group, have demonstrated that the third dose (booster vaccination) of inactivated COVID-19 vaccine induces robust humoral and cellular immune responses. The present study aimed to examine miRNA expression profile in participants who received a homologous third dose of the CoronaVac vaccine. Samples of peripheral blood mononuclear cells (PBMCs) were collected from healthcare volunteers both before and 1–2 weeks after the booster dose. miRNA microarray analysis in a discovery cohort of six volunteers identified 67 miRNAs with differential expression. Subsequently, the expression of six miRNAs related to immune responses was examined in a validation cohort of 31 participants via qRT-PCR. Our results validated the differential expression of miR-25-5p, miR-34c-3p, and miR-206 post-booster, with a significant correlation to the receptor binding domain (RBD)-specific antibody. Bioinformatic analysis suggested that miR-25-5p, miR-34c-3p, and miR-206 may target multiple pathways involved in immune regulation and inflammation. Therefore, our study highlights miR-25-5p, miR-34c-3p, and miR-206 in PBMCs as promising biomarkers for assessing the immune response induced by the booster dose of the CoronaVac vaccine.https://peerj.com/articles/18856.pdfCOVID-19Inactivated vaccineCoronaVacPeripheral blood mononuclear cellsMicroRNAImmune response |
| spellingShingle | Guanguan Qiu Ruoyang Zhang Huifeng Qian Ruoqiong Huang Jie Xia Ruoxi Zang Zhenkai Le Qiang Shu Jianguo Xu Guoping Zheng Jiangmei Wang Altered expression of miRNA profile in peripheral blood mononuclear cells following the third dose of inactivated COVID-19 vaccine PeerJ COVID-19 Inactivated vaccine CoronaVac Peripheral blood mononuclear cells MicroRNA Immune response |
| title | Altered expression of miRNA profile in peripheral blood mononuclear cells following the third dose of inactivated COVID-19 vaccine |
| title_full | Altered expression of miRNA profile in peripheral blood mononuclear cells following the third dose of inactivated COVID-19 vaccine |
| title_fullStr | Altered expression of miRNA profile in peripheral blood mononuclear cells following the third dose of inactivated COVID-19 vaccine |
| title_full_unstemmed | Altered expression of miRNA profile in peripheral blood mononuclear cells following the third dose of inactivated COVID-19 vaccine |
| title_short | Altered expression of miRNA profile in peripheral blood mononuclear cells following the third dose of inactivated COVID-19 vaccine |
| title_sort | altered expression of mirna profile in peripheral blood mononuclear cells following the third dose of inactivated covid 19 vaccine |
| topic | COVID-19 Inactivated vaccine CoronaVac Peripheral blood mononuclear cells MicroRNA Immune response |
| url | https://peerj.com/articles/18856.pdf |
| work_keys_str_mv | AT guanguanqiu alteredexpressionofmirnaprofileinperipheralbloodmononuclearcellsfollowingthethirddoseofinactivatedcovid19vaccine AT ruoyangzhang alteredexpressionofmirnaprofileinperipheralbloodmononuclearcellsfollowingthethirddoseofinactivatedcovid19vaccine AT huifengqian alteredexpressionofmirnaprofileinperipheralbloodmononuclearcellsfollowingthethirddoseofinactivatedcovid19vaccine AT ruoqionghuang alteredexpressionofmirnaprofileinperipheralbloodmononuclearcellsfollowingthethirddoseofinactivatedcovid19vaccine AT jiexia alteredexpressionofmirnaprofileinperipheralbloodmononuclearcellsfollowingthethirddoseofinactivatedcovid19vaccine AT ruoxizang alteredexpressionofmirnaprofileinperipheralbloodmononuclearcellsfollowingthethirddoseofinactivatedcovid19vaccine AT zhenkaile alteredexpressionofmirnaprofileinperipheralbloodmononuclearcellsfollowingthethirddoseofinactivatedcovid19vaccine AT qiangshu alteredexpressionofmirnaprofileinperipheralbloodmononuclearcellsfollowingthethirddoseofinactivatedcovid19vaccine AT jianguoxu alteredexpressionofmirnaprofileinperipheralbloodmononuclearcellsfollowingthethirddoseofinactivatedcovid19vaccine AT guopingzheng alteredexpressionofmirnaprofileinperipheralbloodmononuclearcellsfollowingthethirddoseofinactivatedcovid19vaccine AT jiangmeiwang alteredexpressionofmirnaprofileinperipheralbloodmononuclearcellsfollowingthethirddoseofinactivatedcovid19vaccine |