Rapid Determination of Catechin Content in Black Tea by Fluorescence Spectroscopy
Catechin can effectively prevent the occurrence of cancers due to its strong antioxidant capacity. In this study, the catechin contents of black teas from 12 different regions of south China were investigated using fluorescence spectroscopy. Herein, the catechin contents of various black teas with c...
Saved in:
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2020-01-01
|
| Series: | Journal of Spectroscopy |
| Online Access: | http://dx.doi.org/10.1155/2020/2479612 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832546970006192128 |
|---|---|
| author | Chenxu Du Chaoqun Ma Jiao Gu Lei Li Chun Zhu Lvming Chen Tingyu Wang Guoqing Chen |
| author_facet | Chenxu Du Chaoqun Ma Jiao Gu Lei Li Chun Zhu Lvming Chen Tingyu Wang Guoqing Chen |
| author_sort | Chenxu Du |
| collection | DOAJ |
| description | Catechin can effectively prevent the occurrence of cancers due to its strong antioxidant capacity. In this study, the catechin contents of black teas from 12 different regions of south China were investigated using fluorescence spectroscopy. Herein, the catechin contents of various black teas with constant concentration were determined at the optimal excitation and emission wavelength combining the standard addition method and fluorescence spectroscopy. The results indicated that there was a linear relationship between the obtained concentration and fluorescence intensity, where the R values were all greater than 0.99 and the limit of quantification (LOQ) was 0.02 μg/mL. Furthermore, the content of catechin monomer in the chlorophyll environment was measured under the same experimental conditions to demonstrate the correctness of the above experimental methods. It revealed that the experimental error was about 1.14% compared with the actual content. The current work was proved to be an efficient way to detect fluorescence spectrum through diluting the concentration of tea samples, thereby increasing the determination limit of catechin. |
| format | Article |
| id | doaj-art-bfcbd3c542e44f00863a9c72538eb7bf |
| institution | Kabale University |
| issn | 2314-4920 2314-4939 |
| language | English |
| publishDate | 2020-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Spectroscopy |
| spelling | doaj-art-bfcbd3c542e44f00863a9c72538eb7bf2025-02-03T06:46:31ZengWileyJournal of Spectroscopy2314-49202314-49392020-01-01202010.1155/2020/24796122479612Rapid Determination of Catechin Content in Black Tea by Fluorescence SpectroscopyChenxu Du0Chaoqun Ma1Jiao Gu2Lei Li3Chun Zhu4Lvming Chen5Tingyu Wang6Guoqing Chen7School of Science, Jiangnan University, Wuxi, ChinaSchool of Science, Jiangnan University, Wuxi, ChinaSchool of Science, Jiangnan University, Wuxi, ChinaSchool of Science, Jiangnan University, Wuxi, ChinaSchool of Science, Jiangnan University, Wuxi, ChinaSchool of Science, Jiangnan University, Wuxi, ChinaSchool of Science, Jiangnan University, Wuxi, ChinaSchool of Science, Jiangnan University, Wuxi, ChinaCatechin can effectively prevent the occurrence of cancers due to its strong antioxidant capacity. In this study, the catechin contents of black teas from 12 different regions of south China were investigated using fluorescence spectroscopy. Herein, the catechin contents of various black teas with constant concentration were determined at the optimal excitation and emission wavelength combining the standard addition method and fluorescence spectroscopy. The results indicated that there was a linear relationship between the obtained concentration and fluorescence intensity, where the R values were all greater than 0.99 and the limit of quantification (LOQ) was 0.02 μg/mL. Furthermore, the content of catechin monomer in the chlorophyll environment was measured under the same experimental conditions to demonstrate the correctness of the above experimental methods. It revealed that the experimental error was about 1.14% compared with the actual content. The current work was proved to be an efficient way to detect fluorescence spectrum through diluting the concentration of tea samples, thereby increasing the determination limit of catechin.http://dx.doi.org/10.1155/2020/2479612 |
| spellingShingle | Chenxu Du Chaoqun Ma Jiao Gu Lei Li Chun Zhu Lvming Chen Tingyu Wang Guoqing Chen Rapid Determination of Catechin Content in Black Tea by Fluorescence Spectroscopy Journal of Spectroscopy |
| title | Rapid Determination of Catechin Content in Black Tea by Fluorescence Spectroscopy |
| title_full | Rapid Determination of Catechin Content in Black Tea by Fluorescence Spectroscopy |
| title_fullStr | Rapid Determination of Catechin Content in Black Tea by Fluorescence Spectroscopy |
| title_full_unstemmed | Rapid Determination of Catechin Content in Black Tea by Fluorescence Spectroscopy |
| title_short | Rapid Determination of Catechin Content in Black Tea by Fluorescence Spectroscopy |
| title_sort | rapid determination of catechin content in black tea by fluorescence spectroscopy |
| url | http://dx.doi.org/10.1155/2020/2479612 |
| work_keys_str_mv | AT chenxudu rapiddeterminationofcatechincontentinblackteabyfluorescencespectroscopy AT chaoqunma rapiddeterminationofcatechincontentinblackteabyfluorescencespectroscopy AT jiaogu rapiddeterminationofcatechincontentinblackteabyfluorescencespectroscopy AT leili rapiddeterminationofcatechincontentinblackteabyfluorescencespectroscopy AT chunzhu rapiddeterminationofcatechincontentinblackteabyfluorescencespectroscopy AT lvmingchen rapiddeterminationofcatechincontentinblackteabyfluorescencespectroscopy AT tingyuwang rapiddeterminationofcatechincontentinblackteabyfluorescencespectroscopy AT guoqingchen rapiddeterminationofcatechincontentinblackteabyfluorescencespectroscopy |