Human embryonic stem cells cultured on hydrogels grafted with extracellular matrix protein‐derived peptides with polyethylene glycol joint nanosegments
Abstract Human pluripotent stem cells (hPSCs) can be proliferated on completely synthetic materials under xeno‐free cultivation conditions using biomaterials grafted with extracellular matrix protein (ECM)‐derived peptides. However, cell culture biomaterials grafted with ECM‐derived peptides must be...
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| Format: | Article |
| Language: | English |
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Wiley
2022-12-01
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| Series: | IET Nanobiotechnology |
| Online Access: | https://doi.org/10.1049/nbt2.12091 |
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| author | Abdullah A. Alarfaj Abdurahman H. Hirad Murugan A. Munusamy S. Suresh Kumar Akon Higuchi |
| author_facet | Abdullah A. Alarfaj Abdurahman H. Hirad Murugan A. Munusamy S. Suresh Kumar Akon Higuchi |
| author_sort | Abdullah A. Alarfaj |
| collection | DOAJ |
| description | Abstract Human pluripotent stem cells (hPSCs) can be proliferated on completely synthetic materials under xeno‐free cultivation conditions using biomaterials grafted with extracellular matrix protein (ECM)‐derived peptides. However, cell culture biomaterials grafted with ECM‐derived peptides must be prepared using a high concentration of peptide reaction solution (e.g. 1000 μg/ml), whereas the ECM concentration of the ECM‐coated surface for hPSC culture is typically 5 μg/ml. We designed a polyethylene glycol (PEG) joint nanosegment (linker) to be used between base cell culture biomaterials and bioactive ECM‐derived peptides to enhance the probability of contact between ECM‐derived peptides and cell binding receptors of hPSCs. Vitronectin‐derived peptides with glycine joint nanosegments (GCGG) were conjugated onto poly (vinyl alcohol‐co‐itaconic acid) hydrogels via PEG joint nanosegments, and human embryonic stem cells (hESCs) were cultivated on these hydrogels. hESCs could successfully be cultivated on hydrogels while maintaining their pluripotency and differentiation potential to differentiate into cells that are induced from three germ layers in vitro and in vivo, where only a 50 μg/ml ECM‐derived peptide concentration was used when the PEG joint nanosegments were introduced into peptides that were grafted onto hydrogel surfaces. The joint nanosegments between bioactive peptides and base cell culture biomaterials were found to contribute to efficient hESC attachment and proliferation. |
| format | Article |
| id | doaj-art-bfbe0b208ea14edc9d4d8cfa6ad6a063 |
| institution | Kabale University |
| issn | 1751-8741 1751-875X |
| language | English |
| publishDate | 2022-12-01 |
| publisher | Wiley |
| record_format | Article |
| series | IET Nanobiotechnology |
| spelling | doaj-art-bfbe0b208ea14edc9d4d8cfa6ad6a0632025-02-03T01:29:36ZengWileyIET Nanobiotechnology1751-87411751-875X2022-12-0116929530410.1049/nbt2.12091Human embryonic stem cells cultured on hydrogels grafted with extracellular matrix protein‐derived peptides with polyethylene glycol joint nanosegmentsAbdullah A. Alarfaj0Abdurahman H. Hirad1Murugan A. Munusamy2S. Suresh Kumar3Akon Higuchi4Department of Botany and Microbiology King Saud University Riyadh Saudi ArabiaDepartment of Botany and Microbiology King Saud University Riyadh Saudi ArabiaDepartment of Botany and Microbiology King Saud University Riyadh Saudi ArabiaDepartment of Biotechnology Bharath Institute of Higher Education and Research Chennai‐73 IndiaDepartment of Chemical and Materials Engineering National Central University Taoyuan TaiwanAbstract Human pluripotent stem cells (hPSCs) can be proliferated on completely synthetic materials under xeno‐free cultivation conditions using biomaterials grafted with extracellular matrix protein (ECM)‐derived peptides. However, cell culture biomaterials grafted with ECM‐derived peptides must be prepared using a high concentration of peptide reaction solution (e.g. 1000 μg/ml), whereas the ECM concentration of the ECM‐coated surface for hPSC culture is typically 5 μg/ml. We designed a polyethylene glycol (PEG) joint nanosegment (linker) to be used between base cell culture biomaterials and bioactive ECM‐derived peptides to enhance the probability of contact between ECM‐derived peptides and cell binding receptors of hPSCs. Vitronectin‐derived peptides with glycine joint nanosegments (GCGG) were conjugated onto poly (vinyl alcohol‐co‐itaconic acid) hydrogels via PEG joint nanosegments, and human embryonic stem cells (hESCs) were cultivated on these hydrogels. hESCs could successfully be cultivated on hydrogels while maintaining their pluripotency and differentiation potential to differentiate into cells that are induced from three germ layers in vitro and in vivo, where only a 50 μg/ml ECM‐derived peptide concentration was used when the PEG joint nanosegments were introduced into peptides that were grafted onto hydrogel surfaces. The joint nanosegments between bioactive peptides and base cell culture biomaterials were found to contribute to efficient hESC attachment and proliferation.https://doi.org/10.1049/nbt2.12091 |
| spellingShingle | Abdullah A. Alarfaj Abdurahman H. Hirad Murugan A. Munusamy S. Suresh Kumar Akon Higuchi Human embryonic stem cells cultured on hydrogels grafted with extracellular matrix protein‐derived peptides with polyethylene glycol joint nanosegments IET Nanobiotechnology |
| title | Human embryonic stem cells cultured on hydrogels grafted with extracellular matrix protein‐derived peptides with polyethylene glycol joint nanosegments |
| title_full | Human embryonic stem cells cultured on hydrogels grafted with extracellular matrix protein‐derived peptides with polyethylene glycol joint nanosegments |
| title_fullStr | Human embryonic stem cells cultured on hydrogels grafted with extracellular matrix protein‐derived peptides with polyethylene glycol joint nanosegments |
| title_full_unstemmed | Human embryonic stem cells cultured on hydrogels grafted with extracellular matrix protein‐derived peptides with polyethylene glycol joint nanosegments |
| title_short | Human embryonic stem cells cultured on hydrogels grafted with extracellular matrix protein‐derived peptides with polyethylene glycol joint nanosegments |
| title_sort | human embryonic stem cells cultured on hydrogels grafted with extracellular matrix protein derived peptides with polyethylene glycol joint nanosegments |
| url | https://doi.org/10.1049/nbt2.12091 |
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