Construction of human peritoneal mesothelial cell lines with PKCα gene knockout with the technique of CRISPR/Cas9

Construction of human peritoneal mesothelial cell lines with PKCα gene knockout with the technique of CRISPR/Cas9

Objective To construct an in vitro cell model for elucidating the pathogenesis of peritoneal fibrosis by a knockout of PKCα gene in human peritoneal mesothelial cell line HMRSV5 with the technique of CRISPR/Cas9.Methods Three pairs of sgRNA sequences were designed for exon region of human PKCα gene....

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Bibliographic Details
Main Authors: Wu Zhong-cai, Ma Zu-fu, Liang Wang-qun, Zuo Xue-zhi, Yao Ying, Xu Gang, Wang Le
Format: Article
Language:zho
Published: Editorial Department of Journal of Clinical Nephrology 2021-01-01
Series:Linchuang shenzangbing zazhi
Subjects:
Peritoneal fibrosis
PKC alpha
CRISPR/Cas9
Knockout
Online Access:http://www.lcszb.com/thesisDetails?columnId=57902084&Fpath=home&index=0
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