New Evidence for <i>Cotinus coggygria</i> Scop. Extracts Application in Gastrointestinal Ailments
Background/Objectives: <i>Cotinus coggygria</i> Scop. is traditionally used for treatment of various gastrointestinal ailments. In this study, we investigated the phytochemical profile and biological activities of leaves, bark and flowers extracts of <i>C. coggygria.</i> Meth...
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2025-01-01
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| author | Dejan Stojković Nina Dragičević Marija Ivanov Nevena Gajović Milena Jurišević Ivan Jovanović Marina Tomović Jelena Živković |
| author_facet | Dejan Stojković Nina Dragičević Marija Ivanov Nevena Gajović Milena Jurišević Ivan Jovanović Marina Tomović Jelena Živković |
| author_sort | Dejan Stojković |
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| description | Background/Objectives: <i>Cotinus coggygria</i> Scop. is traditionally used for treatment of various gastrointestinal ailments. In this study, we investigated the phytochemical profile and biological activities of leaves, bark and flowers extracts of <i>C. coggygria.</i> Methods: Phytochemical analysis was performed using HPLC. The antimicrobial activity of water and methanolic extracts of <i>C. coggygria</i> leaves, bark and flowers towards various oral fungal and bacterial strains of clinical origin was tested by use of a microdilution assay. Additionally, their cytotoxic activity was determined against different gastrointestinal carcinoma cell lines (CAL27, FaDu, SW480, HCT116 and MRC-5) in concentrations ranging from 3.125 to 400 µg/mL for 48 and 72 h. Results: The presence of numerous flavonoid and phenolic compounds such as sulfuretin, gallic acid, rutin, hyperoside, and isoquercitrine was detected. <i>Micrococcus luteus</i>, <i>Streptococcus parasanguinis</i>, and <i>Candida tropicalis</i> were the most sensitive microbiological species, with MICs of 0.12 mg/mL for the most effective extracts. Additionally, the cytotoxic activity of the samples against different gastrointestinal carcinoma cell lines (CAL27, FaDu, SW480, HCT116, and MRC-5) was determined in concentrations ranging from 3.125 to 400 µg/mL. Among the tested samples, the methanolic leaf extract exhibited the highest cytotoxic capacity, and the possible mechanism could be related to its inhibitory effect on the release of proinflammatory cytokine in CD4+ cells. Conclusions: The traditional use of <i>C. coggygria</i> for gastrointestinal diseases may be substantiated by its ability to inhibit the growth of harmful microorganisms and its promising cytotoxic properties. The methanolic leaf and flower extracts show significant potential for future clinical applications, and further studies are warranted to explore their mechanisms and applications in medical treatments. |
| format | Article |
| id | doaj-art-bf653ff1f6e642af9408fc75781e0b35 |
| institution | Kabale University |
| issn | 1424-8247 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | MDPI AG |
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| series | Pharmaceuticals |
| spelling | doaj-art-bf653ff1f6e642af9408fc75781e0b352025-01-24T13:45:23ZengMDPI AGPharmaceuticals1424-82472025-01-011819810.3390/ph18010098New Evidence for <i>Cotinus coggygria</i> Scop. Extracts Application in Gastrointestinal AilmentsDejan Stojković0Nina Dragičević1Marija Ivanov2Nevena Gajović3Milena Jurišević4Ivan Jovanović5Marina Tomović6Jelena Živković7Institute for Biological Research “Siniša Stanković”—National Institute of the Republic of Serbia, University of Belgrade, Bulevar despota Stefana 142, 11108 Belgrade, SerbiaDepartment of Pharmacy, Singidunum University, Danijelova 32, 11000 Belgrade, SerbiaInstitute for Biological Research “Siniša Stanković”—National Institute of the Republic of Serbia, University of Belgrade, Bulevar despota Stefana 142, 11108 Belgrade, SerbiaCenter for Molecular Medicine and Stem Cell Research, Faculty of Medical Sciences, University of Kragujevac, Svetozara Markovica 69, 34000 Kragujevac, SerbiaCenter for Molecular Medicine and Stem Cell Research, Faculty of Medical Sciences, University of Kragujevac, Svetozara Markovica 69, 34000 Kragujevac, SerbiaCenter for Molecular Medicine and Stem Cell Research, Faculty of Medical Sciences, University of Kragujevac, Svetozara Markovica 69, 34000 Kragujevac, SerbiaCenter for Molecular Medicine and Stem Cell Research, Faculty of Medical Sciences, University of Kragujevac, Svetozara Markovica 69, 34000 Kragujevac, SerbiaInstitute for Medicinal Plants Research “Dr. Josif Pančić”, Tadeuša Košćuška 1, 11000 Belgrade, SerbiaBackground/Objectives: <i>Cotinus coggygria</i> Scop. is traditionally used for treatment of various gastrointestinal ailments. In this study, we investigated the phytochemical profile and biological activities of leaves, bark and flowers extracts of <i>C. coggygria.</i> Methods: Phytochemical analysis was performed using HPLC. The antimicrobial activity of water and methanolic extracts of <i>C. coggygria</i> leaves, bark and flowers towards various oral fungal and bacterial strains of clinical origin was tested by use of a microdilution assay. Additionally, their cytotoxic activity was determined against different gastrointestinal carcinoma cell lines (CAL27, FaDu, SW480, HCT116 and MRC-5) in concentrations ranging from 3.125 to 400 µg/mL for 48 and 72 h. Results: The presence of numerous flavonoid and phenolic compounds such as sulfuretin, gallic acid, rutin, hyperoside, and isoquercitrine was detected. <i>Micrococcus luteus</i>, <i>Streptococcus parasanguinis</i>, and <i>Candida tropicalis</i> were the most sensitive microbiological species, with MICs of 0.12 mg/mL for the most effective extracts. Additionally, the cytotoxic activity of the samples against different gastrointestinal carcinoma cell lines (CAL27, FaDu, SW480, HCT116, and MRC-5) was determined in concentrations ranging from 3.125 to 400 µg/mL. Among the tested samples, the methanolic leaf extract exhibited the highest cytotoxic capacity, and the possible mechanism could be related to its inhibitory effect on the release of proinflammatory cytokine in CD4+ cells. Conclusions: The traditional use of <i>C. coggygria</i> for gastrointestinal diseases may be substantiated by its ability to inhibit the growth of harmful microorganisms and its promising cytotoxic properties. The methanolic leaf and flower extracts show significant potential for future clinical applications, and further studies are warranted to explore their mechanisms and applications in medical treatments.https://www.mdpi.com/1424-8247/18/1/98<i>Cotinus coggygria</i>flavonoidsphenolic acidsgastrointestinalantimicrobialcytotoxic |
| spellingShingle | Dejan Stojković Nina Dragičević Marija Ivanov Nevena Gajović Milena Jurišević Ivan Jovanović Marina Tomović Jelena Živković New Evidence for <i>Cotinus coggygria</i> Scop. Extracts Application in Gastrointestinal Ailments Pharmaceuticals <i>Cotinus coggygria</i> flavonoids phenolic acids gastrointestinal antimicrobial cytotoxic |
| title | New Evidence for <i>Cotinus coggygria</i> Scop. Extracts Application in Gastrointestinal Ailments |
| title_full | New Evidence for <i>Cotinus coggygria</i> Scop. Extracts Application in Gastrointestinal Ailments |
| title_fullStr | New Evidence for <i>Cotinus coggygria</i> Scop. Extracts Application in Gastrointestinal Ailments |
| title_full_unstemmed | New Evidence for <i>Cotinus coggygria</i> Scop. Extracts Application in Gastrointestinal Ailments |
| title_short | New Evidence for <i>Cotinus coggygria</i> Scop. Extracts Application in Gastrointestinal Ailments |
| title_sort | new evidence for i cotinus coggygria i scop extracts application in gastrointestinal ailments |
| topic | <i>Cotinus coggygria</i> flavonoids phenolic acids gastrointestinal antimicrobial cytotoxic |
| url | https://www.mdpi.com/1424-8247/18/1/98 |
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