Regeneration of Tracheal Tissue in Partial Defects Using Porcine Small Intestinal Submucosa
Background. Surgical correction of tracheal defects is a complex procedure when the gold standard treatment with primary end-to-end anastomosis is not possible. An alternative treatment may be the use of porcine small intestinal submucosa (SIS). It has been used as graft material for bioengineering...
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| Language: | English |
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Wiley
2018-01-01
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| Series: | Stem Cells International |
| Online Access: | http://dx.doi.org/10.1155/2018/5102630 |
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| author | Nelson Bergonse Neto Lianna Ferrari Jorge Julio C. Francisco Bruna Olandoski Erbano Barbara Evelin Gonçalves Barboza Larissa Luvison Gomes da Silva Marcia Olandoski Katherine Athayde Teixeira de Carvalho Luiz Felipe Pinho Moreira Jose Rocha Faria Neto Eltyeb Abdelwahid Luiz Cesar Guarita-Souza |
| author_facet | Nelson Bergonse Neto Lianna Ferrari Jorge Julio C. Francisco Bruna Olandoski Erbano Barbara Evelin Gonçalves Barboza Larissa Luvison Gomes da Silva Marcia Olandoski Katherine Athayde Teixeira de Carvalho Luiz Felipe Pinho Moreira Jose Rocha Faria Neto Eltyeb Abdelwahid Luiz Cesar Guarita-Souza |
| author_sort | Nelson Bergonse Neto |
| collection | DOAJ |
| description | Background. Surgical correction of tracheal defects is a complex procedure when the gold standard treatment with primary end-to-end anastomosis is not possible. An alternative treatment may be the use of porcine small intestinal submucosa (SIS). It has been used as graft material for bioengineering applications and to promote tissue regeneration. The aim of this study was to evaluate whether SIS grafts improved tracheal tissue regeneration in a rabbit model of experimental tracheostomy. Methods. Sixteen rabbits were randomized into two groups. Animals in the control group underwent only surgical tracheostomy, while animals in the SIS group underwent surgical tracheostomy with an SIS graft covering the defect. We examined tissues at the site of tracheostomy 60 days after surgery using histological analysis with hematoxylin and eosin (H&E) staining and analyzed the perimeter and area of the defect with Image-Pro® PLUS 4.5 (Media Cybernetics). Results. The average perimeter and area of the defects were smaller by 15.3% (p=0.034) and 21.8% (p=0.151), respectively, in the SIS group than in the control group. Histological analysis revealed immature cartilage, pseudostratified ciliated epithelium, and connective tissue in 54.5% (p=0.018) of the SIS group, while no cartilaginous regeneration was observed in the control group. Conclusions. Although tracheal SIS engraftment could not prevent stenosis in a rabbit model of tracheal injury, it produced some remarkable changes, efficiently facilitating neovascularization, reepithelialization, and neoformation of immature cartilage. |
| format | Article |
| id | doaj-art-bf3cfe1d71ab4a11a880955ec7560ee0 |
| institution | Kabale University |
| issn | 1687-966X 1687-9678 |
| language | English |
| publishDate | 2018-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Stem Cells International |
| spelling | doaj-art-bf3cfe1d71ab4a11a880955ec7560ee02025-02-03T01:12:08ZengWileyStem Cells International1687-966X1687-96782018-01-01201810.1155/2018/51026305102630Regeneration of Tracheal Tissue in Partial Defects Using Porcine Small Intestinal SubmucosaNelson Bergonse Neto0Lianna Ferrari Jorge1Julio C. Francisco2Bruna Olandoski Erbano3Barbara Evelin Gonçalves Barboza4Larissa Luvison Gomes da Silva5Marcia Olandoski6Katherine Athayde Teixeira de Carvalho7Luiz Felipe Pinho Moreira8Jose Rocha Faria Neto9Eltyeb Abdelwahid10Luiz Cesar Guarita-Souza11Experimental Laboratory of Institute of Biological and Health Sciences of Pontifical Catholic University of Paraná (PUCPR), Rua Imaculada Conceição 1155, 80215-901 Curitiba, PR, BrazilExperimental Laboratory of Institute of Biological and Health Sciences of Pontifical Catholic University of Paraná (PUCPR), Rua Imaculada Conceição 1155, 80215-901 Curitiba, PR, BrazilExperimental Laboratory of Institute of Biological and Health Sciences of Pontifical Catholic University of Paraná (PUCPR), Rua Imaculada Conceição 1155, 80215-901 Curitiba, PR, BrazilExperimental Laboratory of Institute of Biological and Health Sciences of Pontifical Catholic University of Paraná (PUCPR), Rua Imaculada Conceição 1155, 80215-901 Curitiba, PR, BrazilExperimental Laboratory of Institute of Biological and Health Sciences of Pontifical Catholic University of Paraná (PUCPR), Rua Imaculada Conceição 1155, 80215-901 Curitiba, PR, BrazilCenter of Cytopathology of Paraná (CITOPAR), Av. Sete de Setembro 5426, 80240-220 Curitiba, PR, BrazilExperimental Laboratory of Institute of Biological and Health Sciences of Pontifical Catholic University of Paraná (PUCPR), Rua Imaculada Conceição 1155, 80215-901 Curitiba, PR, BrazilCell Therapy and Biotechnology in Regenerative Medicine Research Group, Pelé Pequeno Príncipe Institute, Avenida Silva Jardim 1632, 80250-200 Curitiba, PR, BrazilHeart Institute (InCor), University of São Paulo Medical School, LM, 11 São Paulo, SP, BrazilExperimental Laboratory of Institute of Biological and Health Sciences of Pontifical Catholic University of Paraná (PUCPR), Rua Imaculada Conceição 1155, 80215-901 Curitiba, PR, BrazilFeinberg School of Medicine, Feinberg Cardiovascular Research Institute, Northwestern University, 303 E. Chicago Ave., Chicago, IL 60611, USAExperimental Laboratory of Institute of Biological and Health Sciences of Pontifical Catholic University of Paraná (PUCPR), Rua Imaculada Conceição 1155, 80215-901 Curitiba, PR, BrazilBackground. Surgical correction of tracheal defects is a complex procedure when the gold standard treatment with primary end-to-end anastomosis is not possible. An alternative treatment may be the use of porcine small intestinal submucosa (SIS). It has been used as graft material for bioengineering applications and to promote tissue regeneration. The aim of this study was to evaluate whether SIS grafts improved tracheal tissue regeneration in a rabbit model of experimental tracheostomy. Methods. Sixteen rabbits were randomized into two groups. Animals in the control group underwent only surgical tracheostomy, while animals in the SIS group underwent surgical tracheostomy with an SIS graft covering the defect. We examined tissues at the site of tracheostomy 60 days after surgery using histological analysis with hematoxylin and eosin (H&E) staining and analyzed the perimeter and area of the defect with Image-Pro® PLUS 4.5 (Media Cybernetics). Results. The average perimeter and area of the defects were smaller by 15.3% (p=0.034) and 21.8% (p=0.151), respectively, in the SIS group than in the control group. Histological analysis revealed immature cartilage, pseudostratified ciliated epithelium, and connective tissue in 54.5% (p=0.018) of the SIS group, while no cartilaginous regeneration was observed in the control group. Conclusions. Although tracheal SIS engraftment could not prevent stenosis in a rabbit model of tracheal injury, it produced some remarkable changes, efficiently facilitating neovascularization, reepithelialization, and neoformation of immature cartilage.http://dx.doi.org/10.1155/2018/5102630 |
| spellingShingle | Nelson Bergonse Neto Lianna Ferrari Jorge Julio C. Francisco Bruna Olandoski Erbano Barbara Evelin Gonçalves Barboza Larissa Luvison Gomes da Silva Marcia Olandoski Katherine Athayde Teixeira de Carvalho Luiz Felipe Pinho Moreira Jose Rocha Faria Neto Eltyeb Abdelwahid Luiz Cesar Guarita-Souza Regeneration of Tracheal Tissue in Partial Defects Using Porcine Small Intestinal Submucosa Stem Cells International |
| title | Regeneration of Tracheal Tissue in Partial Defects Using Porcine Small Intestinal Submucosa |
| title_full | Regeneration of Tracheal Tissue in Partial Defects Using Porcine Small Intestinal Submucosa |
| title_fullStr | Regeneration of Tracheal Tissue in Partial Defects Using Porcine Small Intestinal Submucosa |
| title_full_unstemmed | Regeneration of Tracheal Tissue in Partial Defects Using Porcine Small Intestinal Submucosa |
| title_short | Regeneration of Tracheal Tissue in Partial Defects Using Porcine Small Intestinal Submucosa |
| title_sort | regeneration of tracheal tissue in partial defects using porcine small intestinal submucosa |
| url | http://dx.doi.org/10.1155/2018/5102630 |
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