Antioxidative Activity and Inhibition of Key Enzymes Linked to Type-2 Diabetes (α-Glucosidase and α-Amylase) by Khaya Senegalensis
This study evaluated the in vitro antioxidative activity of Khaya senegalensis extracts and inhibitory effects of some solvent fractions on α-glucosidase and α-amylase activities. The stem bark, root and leaf samples of the plant were sequentially extracted with ethyl acetate, ethanol and water and...
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Sciendo
2014-09-01
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| Series: | Acta Pharmaceutica |
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| Online Access: | https://doi.org/10.2478/acph-2014-0025 |
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| author | Ibrahim Mohammed Auwal Koorbanally Neil Anthony Islam Md. Shahidul |
| author_facet | Ibrahim Mohammed Auwal Koorbanally Neil Anthony Islam Md. Shahidul |
| author_sort | Ibrahim Mohammed Auwal |
| collection | DOAJ |
| description | This study evaluated the in vitro antioxidative activity of Khaya senegalensis extracts and inhibitory effects of some solvent fractions on α-glucosidase and α-amylase activities. The stem bark, root and leaf samples of the plant were sequentially extracted with ethyl acetate, ethanol and water and then tested for antioxidative activity. Our findings revealed that the ethanolic extract of the root had the highest antioxidative activity. Solvent-solvent fractionation of the root ethanolic extract yielded a butanol fraction that showed higher antioxidative activity than other fractions. Furthermore, the butanol fraction had significantly higher (p < 0.05) α-glucosidase and α-amylase inhibitory activities with IC50 values of 2.89 ± 0.46 and 97.51 ± 5.72 μg mL-1, respectively. Enzyme kinetic studies indicated that the butanol fraction is a non-competitive inhibitor for α-glucosidase with an inhibition binding constant Ki of 1.30 μg mL-1 and a competitive inhibitor of α-amylase with a Ki of 7.50 μg mL-1. GC-MS analysis revealed that the butanol fraction contained two chromones, p-anilinophenol and 3-ethyl-5-(3- ethyl-(3H)-benzothiazol-2-ylidene)-2-(p-tolylvinylamino)- 4-thiazolidinone. Data obtained in the study suggest that the butanol fraction derived from the ethanolic extract of K. senegalensis root possessed excellent antioxidative as well as α-glucosidase and a-amylase inhibitory activities while chromones and/or p-anilinophenol could be the main bioactive compounds responsible for the observed activities. |
| format | Article |
| id | doaj-art-bf38f46a397f48c790dcd074be7c56cc |
| institution | Kabale University |
| issn | 1846-9558 |
| language | English |
| publishDate | 2014-09-01 |
| publisher | Sciendo |
| record_format | Article |
| series | Acta Pharmaceutica |
| spelling | doaj-art-bf38f46a397f48c790dcd074be7c56cc2025-02-02T15:49:12ZengSciendoActa Pharmaceutica1846-95582014-09-0164331132410.2478/acph-2014-0025acph-2014-0025Antioxidative Activity and Inhibition of Key Enzymes Linked to Type-2 Diabetes (α-Glucosidase and α-Amylase) by Khaya SenegalensisIbrahim Mohammed Auwal0Koorbanally Neil Anthony1Islam Md. Shahidul2School of Life Sciences and Department of Biochemistry Ahmadu Bello University, Zaria NigeriaSchool of Chemistry and Physics University of KwaZulu-Natal (Westville Campus) Durban, 4000, South AfricaSchool of Life SciencesThis study evaluated the in vitro antioxidative activity of Khaya senegalensis extracts and inhibitory effects of some solvent fractions on α-glucosidase and α-amylase activities. The stem bark, root and leaf samples of the plant were sequentially extracted with ethyl acetate, ethanol and water and then tested for antioxidative activity. Our findings revealed that the ethanolic extract of the root had the highest antioxidative activity. Solvent-solvent fractionation of the root ethanolic extract yielded a butanol fraction that showed higher antioxidative activity than other fractions. Furthermore, the butanol fraction had significantly higher (p < 0.05) α-glucosidase and α-amylase inhibitory activities with IC50 values of 2.89 ± 0.46 and 97.51 ± 5.72 μg mL-1, respectively. Enzyme kinetic studies indicated that the butanol fraction is a non-competitive inhibitor for α-glucosidase with an inhibition binding constant Ki of 1.30 μg mL-1 and a competitive inhibitor of α-amylase with a Ki of 7.50 μg mL-1. GC-MS analysis revealed that the butanol fraction contained two chromones, p-anilinophenol and 3-ethyl-5-(3- ethyl-(3H)-benzothiazol-2-ylidene)-2-(p-tolylvinylamino)- 4-thiazolidinone. Data obtained in the study suggest that the butanol fraction derived from the ethanolic extract of K. senegalensis root possessed excellent antioxidative as well as α-glucosidase and a-amylase inhibitory activities while chromones and/or p-anilinophenol could be the main bioactive compounds responsible for the observed activities.https://doi.org/10.2478/acph-2014-0025khaya senegalensis (meliaceae)type-2 diabetesα-glucosidaseα-amylaseoxidative stress |
| spellingShingle | Ibrahim Mohammed Auwal Koorbanally Neil Anthony Islam Md. Shahidul Antioxidative Activity and Inhibition of Key Enzymes Linked to Type-2 Diabetes (α-Glucosidase and α-Amylase) by Khaya Senegalensis Acta Pharmaceutica khaya senegalensis (meliaceae) type-2 diabetes α-glucosidase α-amylase oxidative stress |
| title | Antioxidative Activity and Inhibition of Key Enzymes Linked to Type-2 Diabetes (α-Glucosidase and α-Amylase) by Khaya Senegalensis |
| title_full | Antioxidative Activity and Inhibition of Key Enzymes Linked to Type-2 Diabetes (α-Glucosidase and α-Amylase) by Khaya Senegalensis |
| title_fullStr | Antioxidative Activity and Inhibition of Key Enzymes Linked to Type-2 Diabetes (α-Glucosidase and α-Amylase) by Khaya Senegalensis |
| title_full_unstemmed | Antioxidative Activity and Inhibition of Key Enzymes Linked to Type-2 Diabetes (α-Glucosidase and α-Amylase) by Khaya Senegalensis |
| title_short | Antioxidative Activity and Inhibition of Key Enzymes Linked to Type-2 Diabetes (α-Glucosidase and α-Amylase) by Khaya Senegalensis |
| title_sort | antioxidative activity and inhibition of key enzymes linked to type 2 diabetes α glucosidase and α amylase by khaya senegalensis |
| topic | khaya senegalensis (meliaceae) type-2 diabetes α-glucosidase α-amylase oxidative stress |
| url | https://doi.org/10.2478/acph-2014-0025 |
| work_keys_str_mv | AT ibrahimmohammedauwal antioxidativeactivityandinhibitionofkeyenzymeslinkedtotype2diabetesaglucosidaseandaamylasebykhayasenegalensis AT koorbanallyneilanthony antioxidativeactivityandinhibitionofkeyenzymeslinkedtotype2diabetesaglucosidaseandaamylasebykhayasenegalensis AT islammdshahidul antioxidativeactivityandinhibitionofkeyenzymeslinkedtotype2diabetesaglucosidaseandaamylasebykhayasenegalensis |