Chitosan Hydrogels for Chondroitin Sulphate Controlled Release: An Analytical Characterization
This paper provides an analytical characterization of chitosan scaffolds obtained by freeze-gelation toward the uptake and the controlled release of chondroitin sulphate (CS), as cartilage repair agent, under different pH conditions. Scanning electron microscopy (SEM), attenuated total reflectance-F...
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| Format: | Article |
| Language: | English |
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Wiley
2014-01-01
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| Series: | Journal of Analytical Methods in Chemistry |
| Online Access: | http://dx.doi.org/10.1155/2014/808703 |
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| author | Annalisa Bianchera Enrico Salomi Matteo Pezzanera Elisabeth Ruwet Ruggero Bettini Lisa Elviri |
| author_facet | Annalisa Bianchera Enrico Salomi Matteo Pezzanera Elisabeth Ruwet Ruggero Bettini Lisa Elviri |
| author_sort | Annalisa Bianchera |
| collection | DOAJ |
| description | This paper provides an analytical characterization of chitosan scaffolds obtained by freeze-gelation toward the uptake and the controlled release of chondroitin sulphate (CS), as cartilage repair agent, under different pH conditions. Scanning electron microscopy (SEM), attenuated total reflectance-Fourier transform infrared spectroscopy (ATR-FTIR), and liquid chromatography-UV spectrophotometry (LC-UV) techniques were exploited to obtain qualitative and quantitative descriptions of polymer and drug behaviour in the biomaterial. As for morphology, SEM analysis allowed the evaluation of scaffold porosity in terms of pore size and distribution both at the surface (Feret diameter 58±19 μm) and on the cross section (Feret diameter 106±51 μm). LC and ATR-FTIR evidenced a pH-dependent CS loading and release behaviour, strongly highlighting the role of electrostatic forces on chitosan/chondroitin sulphate interactions. |
| format | Article |
| id | doaj-art-bf380cc2e7dc465bb51d32a48f364529 |
| institution | Kabale University |
| issn | 2090-8865 2090-8873 |
| language | English |
| publishDate | 2014-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Analytical Methods in Chemistry |
| spelling | doaj-art-bf380cc2e7dc465bb51d32a48f3645292025-02-03T01:01:15ZengWileyJournal of Analytical Methods in Chemistry2090-88652090-88732014-01-01201410.1155/2014/808703808703Chitosan Hydrogels for Chondroitin Sulphate Controlled Release: An Analytical CharacterizationAnnalisa Bianchera0Enrico Salomi1Matteo Pezzanera2Elisabeth Ruwet3Ruggero Bettini4Lisa Elviri5Department of Pharmacy, University of Parma, Parco Area delle Scienze 27/A, 43124 Parma, ItalyDepartment of Pharmacy, University of Parma, Parco Area delle Scienze 27/A, 43124 Parma, ItalyDepartment of Pharmacy, University of Parma, Parco Area delle Scienze 27/A, 43124 Parma, ItalyDepartment of Pharmacy, University of Parma, Parco Area delle Scienze 27/A, 43124 Parma, ItalyDepartment of Pharmacy, University of Parma, Parco Area delle Scienze 27/A, 43124 Parma, ItalyDepartment of Pharmacy, University of Parma, Parco Area delle Scienze 27/A, 43124 Parma, ItalyThis paper provides an analytical characterization of chitosan scaffolds obtained by freeze-gelation toward the uptake and the controlled release of chondroitin sulphate (CS), as cartilage repair agent, under different pH conditions. Scanning electron microscopy (SEM), attenuated total reflectance-Fourier transform infrared spectroscopy (ATR-FTIR), and liquid chromatography-UV spectrophotometry (LC-UV) techniques were exploited to obtain qualitative and quantitative descriptions of polymer and drug behaviour in the biomaterial. As for morphology, SEM analysis allowed the evaluation of scaffold porosity in terms of pore size and distribution both at the surface (Feret diameter 58±19 μm) and on the cross section (Feret diameter 106±51 μm). LC and ATR-FTIR evidenced a pH-dependent CS loading and release behaviour, strongly highlighting the role of electrostatic forces on chitosan/chondroitin sulphate interactions.http://dx.doi.org/10.1155/2014/808703 |
| spellingShingle | Annalisa Bianchera Enrico Salomi Matteo Pezzanera Elisabeth Ruwet Ruggero Bettini Lisa Elviri Chitosan Hydrogels for Chondroitin Sulphate Controlled Release: An Analytical Characterization Journal of Analytical Methods in Chemistry |
| title | Chitosan Hydrogels for Chondroitin Sulphate Controlled Release: An Analytical Characterization |
| title_full | Chitosan Hydrogels for Chondroitin Sulphate Controlled Release: An Analytical Characterization |
| title_fullStr | Chitosan Hydrogels for Chondroitin Sulphate Controlled Release: An Analytical Characterization |
| title_full_unstemmed | Chitosan Hydrogels for Chondroitin Sulphate Controlled Release: An Analytical Characterization |
| title_short | Chitosan Hydrogels for Chondroitin Sulphate Controlled Release: An Analytical Characterization |
| title_sort | chitosan hydrogels for chondroitin sulphate controlled release an analytical characterization |
| url | http://dx.doi.org/10.1155/2014/808703 |
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