Polysaccharide Fraction Isolated from <i>Saccharina japonica</i> Exhibits Anti-Cancer Effects Through Immunostimulating Activities
The present research aimed to assess the anti-cancer effects of the polysaccharide fraction (SJP) isolated from <i>Saccharina japonica</i>. The release of immune-activating cytokines, including IL-6, IL-12, and TNF-α, was markedly stimulated by the SJP in a concentration-dependent manner...
Saved in:
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2025-01-01
|
| Series: | Marine Drugs |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1660-3397/23/1/38 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832588086832267264 |
|---|---|
| author | Min Seung Park Seung-U Son Tae Eun Kim Se Hyun Shim Bong-Keun Jang Sunyoung Park Kwang-Soon Shin |
| author_facet | Min Seung Park Seung-U Son Tae Eun Kim Se Hyun Shim Bong-Keun Jang Sunyoung Park Kwang-Soon Shin |
| author_sort | Min Seung Park |
| collection | DOAJ |
| description | The present research aimed to assess the anti-cancer effects of the polysaccharide fraction (SJP) isolated from <i>Saccharina japonica</i>. The release of immune-activating cytokines, including IL-6, IL-12, and TNF-α, was markedly stimulated by the SJP in a concentration-dependent manner within the range of 1 to 100 µg/mL. Furthermore, the prophylactic intravenous (<i>p.i.v.</i>) and per os (<i>p.p.o.</i>) injection of SJP boosted the cytolytic activity mediated by NK cells and CTLs against tumor cells. In a study involving Colon26-M3.1 carcinoma as a lung cancer model, both <i>p.i.v.</i> and <i>p.p.o.</i> exhibited significant anti-lung-cancer effects. Notably, <i>p.i.v.</i> and <i>p.p.o.</i> administration of SJP at a dose of 50 mg/kg reduced tumor colonies by 84% and 40%, respectively, compared to the control. Moreover, the anti-lung-cancer effects of SJP remained substantial, even when NK cell function was inhibited using anti-asialo-GM1. Fractionation with CaCl<sub>2</sub> suggested that SJP is a mixture of alginate and fucoidan. The fucoidan fraction stimulated the immune response of macrophages more strongly than the alginate fraction. Consequently, this finding suggested that SJP from <i>S. japonica</i> possesses remarkable anti-cancer effects through the activation of various immunocytes. In addition, this finding indicates that the potent biological activity of SJP may be attributed to fucoidan. |
| format | Article |
| id | doaj-art-bf05edb300354a49bb89d69796fc09d2 |
| institution | Kabale University |
| issn | 1660-3397 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Marine Drugs |
| spelling | doaj-art-bf05edb300354a49bb89d69796fc09d22025-01-24T13:39:35ZengMDPI AGMarine Drugs1660-33972025-01-012313810.3390/md23010038Polysaccharide Fraction Isolated from <i>Saccharina japonica</i> Exhibits Anti-Cancer Effects Through Immunostimulating ActivitiesMin Seung Park0Seung-U Son1Tae Eun Kim2Se Hyun Shim3Bong-Keun Jang4Sunyoung Park5Kwang-Soon Shin6Department of Food Science and Biotechnology, Kyonggi University, Suwon 16227, Republic of KoreaDepartment of Food Science and Biotechnology, Kyonggi University, Suwon 16227, Republic of KoreaDepartment of Food Science and Biotechnology, Kyonggi University, Suwon 16227, Republic of KoreaDepartment of Food Science and Biotechnology, Kyonggi University, Suwon 16227, Republic of KoreaJBKLAB, Inc., Daejeon 34013, Republic of KoreaJBKLAB, Inc., Daejeon 34013, Republic of KoreaDepartment of Food Science and Biotechnology, Kyonggi University, Suwon 16227, Republic of KoreaThe present research aimed to assess the anti-cancer effects of the polysaccharide fraction (SJP) isolated from <i>Saccharina japonica</i>. The release of immune-activating cytokines, including IL-6, IL-12, and TNF-α, was markedly stimulated by the SJP in a concentration-dependent manner within the range of 1 to 100 µg/mL. Furthermore, the prophylactic intravenous (<i>p.i.v.</i>) and per os (<i>p.p.o.</i>) injection of SJP boosted the cytolytic activity mediated by NK cells and CTLs against tumor cells. In a study involving Colon26-M3.1 carcinoma as a lung cancer model, both <i>p.i.v.</i> and <i>p.p.o.</i> exhibited significant anti-lung-cancer effects. Notably, <i>p.i.v.</i> and <i>p.p.o.</i> administration of SJP at a dose of 50 mg/kg reduced tumor colonies by 84% and 40%, respectively, compared to the control. Moreover, the anti-lung-cancer effects of SJP remained substantial, even when NK cell function was inhibited using anti-asialo-GM1. Fractionation with CaCl<sub>2</sub> suggested that SJP is a mixture of alginate and fucoidan. The fucoidan fraction stimulated the immune response of macrophages more strongly than the alginate fraction. Consequently, this finding suggested that SJP from <i>S. japonica</i> possesses remarkable anti-cancer effects through the activation of various immunocytes. In addition, this finding indicates that the potent biological activity of SJP may be attributed to fucoidan.https://www.mdpi.com/1660-3397/23/1/38<i>Saccharina japonica</i>polysaccharidefucoidanimmunostimulatinganti-cancerseparation |
| spellingShingle | Min Seung Park Seung-U Son Tae Eun Kim Se Hyun Shim Bong-Keun Jang Sunyoung Park Kwang-Soon Shin Polysaccharide Fraction Isolated from <i>Saccharina japonica</i> Exhibits Anti-Cancer Effects Through Immunostimulating Activities Marine Drugs <i>Saccharina japonica</i> polysaccharide fucoidan immunostimulating anti-cancer separation |
| title | Polysaccharide Fraction Isolated from <i>Saccharina japonica</i> Exhibits Anti-Cancer Effects Through Immunostimulating Activities |
| title_full | Polysaccharide Fraction Isolated from <i>Saccharina japonica</i> Exhibits Anti-Cancer Effects Through Immunostimulating Activities |
| title_fullStr | Polysaccharide Fraction Isolated from <i>Saccharina japonica</i> Exhibits Anti-Cancer Effects Through Immunostimulating Activities |
| title_full_unstemmed | Polysaccharide Fraction Isolated from <i>Saccharina japonica</i> Exhibits Anti-Cancer Effects Through Immunostimulating Activities |
| title_short | Polysaccharide Fraction Isolated from <i>Saccharina japonica</i> Exhibits Anti-Cancer Effects Through Immunostimulating Activities |
| title_sort | polysaccharide fraction isolated from i saccharina japonica i exhibits anti cancer effects through immunostimulating activities |
| topic | <i>Saccharina japonica</i> polysaccharide fucoidan immunostimulating anti-cancer separation |
| url | https://www.mdpi.com/1660-3397/23/1/38 |
| work_keys_str_mv | AT minseungpark polysaccharidefractionisolatedfromisaccharinajaponicaiexhibitsanticancereffectsthroughimmunostimulatingactivities AT seunguson polysaccharidefractionisolatedfromisaccharinajaponicaiexhibitsanticancereffectsthroughimmunostimulatingactivities AT taeeunkim polysaccharidefractionisolatedfromisaccharinajaponicaiexhibitsanticancereffectsthroughimmunostimulatingactivities AT sehyunshim polysaccharidefractionisolatedfromisaccharinajaponicaiexhibitsanticancereffectsthroughimmunostimulatingactivities AT bongkeunjang polysaccharidefractionisolatedfromisaccharinajaponicaiexhibitsanticancereffectsthroughimmunostimulatingactivities AT sunyoungpark polysaccharidefractionisolatedfromisaccharinajaponicaiexhibitsanticancereffectsthroughimmunostimulatingactivities AT kwangsoonshin polysaccharidefractionisolatedfromisaccharinajaponicaiexhibitsanticancereffectsthroughimmunostimulatingactivities |