A Full-Length Reference Floral Transcriptome of Boehmeria tricuspis Provides Insights into Apomeiosis and Polyploidy
Boehmeria tricuspis (Hance) Makino constitutes a hardy herbaceous or shrubby perennial native to East Asia that includes different ploidy levels and reproductive modes (diplosporous to sexual). Although several apomeiosis-associated genes have been described, the genetic control and molecular mechan...
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| Format: | Article |
| Language: | English |
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Wiley
2019-01-01
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| Series: | International Journal of Genomics |
| Online Access: | http://dx.doi.org/10.1155/2019/4025747 |
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| author | Qing Tang Ying Xu Canhui Deng Chaohua Cheng Zhigang Dai Zemao Yang Chan Liu Jianguang Su |
| author_facet | Qing Tang Ying Xu Canhui Deng Chaohua Cheng Zhigang Dai Zemao Yang Chan Liu Jianguang Su |
| author_sort | Qing Tang |
| collection | DOAJ |
| description | Boehmeria tricuspis (Hance) Makino constitutes a hardy herbaceous or shrubby perennial native to East Asia that includes different ploidy levels and reproductive modes (diplosporous to sexual). Although several apomeiosis-associated genes have been described, the genetic control and molecular mechanisms underlying apomeiosis remain poorly understood. Moreover, the basis of the correlation between polyploidy and apomixis has not yet been clarified. We utilized long-read sequencing to produce a full-length reference floral transcriptome of B. tricuspis. Based on the generated database, gene expression of the female flowers of different ploidy levels and reproductive mode cytotypes was compared. Overall, 1,387 genes related to apomeiosis, 217 genes related to ploidy, and 9 genes associated with both apomixis and ploidy were identified. Gene Ontology analyses of this set of transcripts indicated reproductive genes, especially those related to “cell differentiation” and “cell cycle process,” as significant factors regulating apomeiosis. Furthermore, our results suggested that different expressions of stress response genes might be important in the preparation for apomeiosis transition. In addition, our observations indicated that the expression of apomeiosis may not depend on polyploidy but rather on deregulation of the sexual pathway in B. tricuspis. |
| format | Article |
| id | doaj-art-bee75ee273114dda95bd8c70aa22eef2 |
| institution | Kabale University |
| issn | 2314-436X 2314-4378 |
| language | English |
| publishDate | 2019-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | International Journal of Genomics |
| spelling | doaj-art-bee75ee273114dda95bd8c70aa22eef22025-02-03T05:57:37ZengWileyInternational Journal of Genomics2314-436X2314-43782019-01-01201910.1155/2019/40257474025747A Full-Length Reference Floral Transcriptome of Boehmeria tricuspis Provides Insights into Apomeiosis and PolyploidyQing Tang0Ying Xu1Canhui Deng2Chaohua Cheng3Zhigang Dai4Zemao Yang5Chan Liu6Jianguang Su7Institute of Bast Fiber Crops, Chinese Academy of Agricultural Sciences, Changsha, 410205 Hunan, ChinaInstitute of Bast Fiber Crops, Chinese Academy of Agricultural Sciences, Changsha, 410205 Hunan, ChinaInstitute of Bast Fiber Crops, Chinese Academy of Agricultural Sciences, Changsha, 410205 Hunan, ChinaInstitute of Bast Fiber Crops, Chinese Academy of Agricultural Sciences, Changsha, 410205 Hunan, ChinaInstitute of Bast Fiber Crops, Chinese Academy of Agricultural Sciences, Changsha, 410205 Hunan, ChinaInstitute of Bast Fiber Crops, Chinese Academy of Agricultural Sciences, Changsha, 410205 Hunan, ChinaInstitute of Bast Fiber Crops, Chinese Academy of Agricultural Sciences, Changsha, 410205 Hunan, ChinaInstitute of Bast Fiber Crops, Chinese Academy of Agricultural Sciences, Changsha, 410205 Hunan, ChinaBoehmeria tricuspis (Hance) Makino constitutes a hardy herbaceous or shrubby perennial native to East Asia that includes different ploidy levels and reproductive modes (diplosporous to sexual). Although several apomeiosis-associated genes have been described, the genetic control and molecular mechanisms underlying apomeiosis remain poorly understood. Moreover, the basis of the correlation between polyploidy and apomixis has not yet been clarified. We utilized long-read sequencing to produce a full-length reference floral transcriptome of B. tricuspis. Based on the generated database, gene expression of the female flowers of different ploidy levels and reproductive mode cytotypes was compared. Overall, 1,387 genes related to apomeiosis, 217 genes related to ploidy, and 9 genes associated with both apomixis and ploidy were identified. Gene Ontology analyses of this set of transcripts indicated reproductive genes, especially those related to “cell differentiation” and “cell cycle process,” as significant factors regulating apomeiosis. Furthermore, our results suggested that different expressions of stress response genes might be important in the preparation for apomeiosis transition. In addition, our observations indicated that the expression of apomeiosis may not depend on polyploidy but rather on deregulation of the sexual pathway in B. tricuspis.http://dx.doi.org/10.1155/2019/4025747 |
| spellingShingle | Qing Tang Ying Xu Canhui Deng Chaohua Cheng Zhigang Dai Zemao Yang Chan Liu Jianguang Su A Full-Length Reference Floral Transcriptome of Boehmeria tricuspis Provides Insights into Apomeiosis and Polyploidy International Journal of Genomics |
| title | A Full-Length Reference Floral Transcriptome of Boehmeria tricuspis Provides Insights into Apomeiosis and Polyploidy |
| title_full | A Full-Length Reference Floral Transcriptome of Boehmeria tricuspis Provides Insights into Apomeiosis and Polyploidy |
| title_fullStr | A Full-Length Reference Floral Transcriptome of Boehmeria tricuspis Provides Insights into Apomeiosis and Polyploidy |
| title_full_unstemmed | A Full-Length Reference Floral Transcriptome of Boehmeria tricuspis Provides Insights into Apomeiosis and Polyploidy |
| title_short | A Full-Length Reference Floral Transcriptome of Boehmeria tricuspis Provides Insights into Apomeiosis and Polyploidy |
| title_sort | full length reference floral transcriptome of boehmeria tricuspis provides insights into apomeiosis and polyploidy |
| url | http://dx.doi.org/10.1155/2019/4025747 |
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