A strain of Talaromyces assiutensis provides multiple protection effects against insect pests and a fungal pathogen after endophytic settlement in soybean plants
Talaromyces assiutensis was reported as an endophytic fungi of plant, with antimicrobial and anticancer properties; however, it has never been reported as an entomopathogenic fungus (EPF). Herein, an EPF strain was isolated from diseased larvae of Spodoptera litura in a soybean field. The purified i...
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Elsevier
2025-02-01
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| Series: | Biological Control |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S1049964425000131 |
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| author | Li Sui Yang Lu Ke Cheng Yifan Tian Zhiming Liu Zhao Xie Zhengkun Zhang Qiyun Li |
| author_facet | Li Sui Yang Lu Ke Cheng Yifan Tian Zhiming Liu Zhao Xie Zhengkun Zhang Qiyun Li |
| author_sort | Li Sui |
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| description | Talaromyces assiutensis was reported as an endophytic fungi of plant, with antimicrobial and anticancer properties; however, it has never been reported as an entomopathogenic fungus (EPF). Herein, an EPF strain was isolated from diseased larvae of Spodoptera litura in a soybean field. The purified isolate was identified as T. assiutensis and designated TaS1GZL-1. Its pathogenicity towards five insect pests belonging to Lepidoptera and Hemiptera, as well as the effect of temperature on its growth and pathogenicity against insects were measured. In addition, its control efficiency against major soybean insect pests and phytopathogenicity were also evaluated after plant colonization. The results showed that TaS1GZL-1 had strong pathogenicity towards five insect pests, and there was no negative effect on the strain growth rate and the corrected mortality rate toward insect pests at 40 °C. Furthermore, this EPF strain could not only inhibit the growth of Sclerotinia sclerotiorum in vitro, but also colonized soybean plants as an endophyte via root irrigation. TaS1GZL-1 colonization upregulated the expression levels of 12 genes related to defense pathways in soybean lateral roots, thereby inducing plant tolerance against phytopathogen infection, as well as disrupting the feeding selectivity of S. litura larvae. Semi-field experiments verified that TaS1GZL-1 had practical control effects on S. litura larvae and sclerotinia disease. This is the first record of a natural infection of insect pests by T. assiutensis, providing new insights into its ecological function. Thus, TaS1GZL-1 is an EPF strain that directly kills a broad-spectrum of insect pests and induces plant tolerance against biotic stress caused by phytopathogens and insect pest after endophytic settlement in soybean plants. |
| format | Article |
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| institution | Kabale University |
| issn | 1049-9644 |
| language | English |
| publishDate | 2025-02-01 |
| publisher | Elsevier |
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| series | Biological Control |
| spelling | doaj-art-bee0c3b7d0c64d5ca35acdd71e7cbcfd2025-02-05T04:31:12ZengElsevierBiological Control1049-96442025-02-01201105703A strain of Talaromyces assiutensis provides multiple protection effects against insect pests and a fungal pathogen after endophytic settlement in soybean plantsLi Sui0Yang Lu1Ke Cheng2Yifan Tian3Zhiming Liu4Zhao Xie5Zhengkun Zhang6Qiyun Li7Institute of Plant Protection, Jilin Academy of Agricultural Sciences, Key Laboratory of Integrated Pest Management on Crops in Northeast China, Ministry of Agriculture and Rural Areas, Changchun 130033, ChinaInstitute of Plant Protection, Jilin Academy of Agricultural Sciences, Key Laboratory of Integrated Pest Management on Crops in Northeast China, Ministry of Agriculture and Rural Areas, Changchun 130033, ChinaInstitute of Plant Protection, Jilin Academy of Agricultural Sciences, Key Laboratory of Integrated Pest Management on Crops in Northeast China, Ministry of Agriculture and Rural Areas, Changchun 130033, ChinaInstitute of Plant Protection, Jilin Academy of Agricultural Sciences, Key Laboratory of Integrated Pest Management on Crops in Northeast China, Ministry of Agriculture and Rural Areas, Changchun 130033, China; Department of Entomology, College of Plant Protection, China Agricultural University, Beijing 100193, ChinaInstitute of Plant Protection, Jilin Academy of Agricultural Sciences, Key Laboratory of Integrated Pest Management on Crops in Northeast China, Ministry of Agriculture and Rural Areas, Changchun 130033, ChinaInstitute of Plant Protection, Jilin Academy of Agricultural Sciences, Key Laboratory of Integrated Pest Management on Crops in Northeast China, Ministry of Agriculture and Rural Areas, Changchun 130033, ChinaInstitute of Plant Protection, Jilin Academy of Agricultural Sciences, Key Laboratory of Integrated Pest Management on Crops in Northeast China, Ministry of Agriculture and Rural Areas, Changchun 130033, China; Corresponding authors at: Institute of Plant Protection, Jilin Academy of Agricultural Sciences, Key Laboratory of Integrated Pest Management on Crops in Northeast China, Ministry of Agriculture and Rural Areas, Changchun 130033, China.Institute of Plant Protection, Jilin Academy of Agricultural Sciences, Key Laboratory of Integrated Pest Management on Crops in Northeast China, Ministry of Agriculture and Rural Areas, Changchun 130033, China; Department of Entomology, College of Plant Protection, China Agricultural University, Beijing 100193, China; Jilin Agricultural University, Jilin, 132109, China; Corresponding authors at: Institute of Plant Protection, Jilin Academy of Agricultural Sciences, Key Laboratory of Integrated Pest Management on Crops in Northeast China, Ministry of Agriculture and Rural Areas, Changchun 130033, China.Talaromyces assiutensis was reported as an endophytic fungi of plant, with antimicrobial and anticancer properties; however, it has never been reported as an entomopathogenic fungus (EPF). Herein, an EPF strain was isolated from diseased larvae of Spodoptera litura in a soybean field. The purified isolate was identified as T. assiutensis and designated TaS1GZL-1. Its pathogenicity towards five insect pests belonging to Lepidoptera and Hemiptera, as well as the effect of temperature on its growth and pathogenicity against insects were measured. In addition, its control efficiency against major soybean insect pests and phytopathogenicity were also evaluated after plant colonization. The results showed that TaS1GZL-1 had strong pathogenicity towards five insect pests, and there was no negative effect on the strain growth rate and the corrected mortality rate toward insect pests at 40 °C. Furthermore, this EPF strain could not only inhibit the growth of Sclerotinia sclerotiorum in vitro, but also colonized soybean plants as an endophyte via root irrigation. TaS1GZL-1 colonization upregulated the expression levels of 12 genes related to defense pathways in soybean lateral roots, thereby inducing plant tolerance against phytopathogen infection, as well as disrupting the feeding selectivity of S. litura larvae. Semi-field experiments verified that TaS1GZL-1 had practical control effects on S. litura larvae and sclerotinia disease. This is the first record of a natural infection of insect pests by T. assiutensis, providing new insights into its ecological function. Thus, TaS1GZL-1 is an EPF strain that directly kills a broad-spectrum of insect pests and induces plant tolerance against biotic stress caused by phytopathogens and insect pest after endophytic settlement in soybean plants.http://www.sciencedirect.com/science/article/pii/S1049964425000131Entomopathogenic fungiTalaromyces assiutensisBroad-spectrum pathogenicityEndogenous colonizationBiocontrol |
| spellingShingle | Li Sui Yang Lu Ke Cheng Yifan Tian Zhiming Liu Zhao Xie Zhengkun Zhang Qiyun Li A strain of Talaromyces assiutensis provides multiple protection effects against insect pests and a fungal pathogen after endophytic settlement in soybean plants Biological Control Entomopathogenic fungi Talaromyces assiutensis Broad-spectrum pathogenicity Endogenous colonization Biocontrol |
| title | A strain of Talaromyces assiutensis provides multiple protection effects against insect pests and a fungal pathogen after endophytic settlement in soybean plants |
| title_full | A strain of Talaromyces assiutensis provides multiple protection effects against insect pests and a fungal pathogen after endophytic settlement in soybean plants |
| title_fullStr | A strain of Talaromyces assiutensis provides multiple protection effects against insect pests and a fungal pathogen after endophytic settlement in soybean plants |
| title_full_unstemmed | A strain of Talaromyces assiutensis provides multiple protection effects against insect pests and a fungal pathogen after endophytic settlement in soybean plants |
| title_short | A strain of Talaromyces assiutensis provides multiple protection effects against insect pests and a fungal pathogen after endophytic settlement in soybean plants |
| title_sort | strain of talaromyces assiutensis provides multiple protection effects against insect pests and a fungal pathogen after endophytic settlement in soybean plants |
| topic | Entomopathogenic fungi Talaromyces assiutensis Broad-spectrum pathogenicity Endogenous colonization Biocontrol |
| url | http://www.sciencedirect.com/science/article/pii/S1049964425000131 |
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