Laxative Effect of Lacticaseibacillus rhamnosus Glory LG12: from the Perspective of the Stem Cell Factor/C-kit Signaling Pathway
Purpose: To explore the laxative effect of Lacticaseibacillus rhamnosus Glory LG12 from the perspective of the stem cell factor (SCF)/C-kit signaling pathway. Methods: Male ICR mice were randomly divided into five groups: control, model, low- (1.5 × 106 CFU/mouse), medium- (1.5 × 107 CFU/mouse), and...
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China Food Publishing Company
2025-02-01
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| Series: | Shipin Kexue |
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| Online Access: | https://www.spkx.net.cn/fileup/1002-6630/PDF/2025-46-3-003.pdf |
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| author | XIANG Fangqin, LI Yuxing, LI Bailiang |
| author_facet | XIANG Fangqin, LI Yuxing, LI Bailiang |
| author_sort | XIANG Fangqin, LI Yuxing, LI Bailiang |
| collection | DOAJ |
| description | Purpose: To explore the laxative effect of Lacticaseibacillus rhamnosus Glory LG12 from the perspective of the stem cell factor (SCF)/C-kit signaling pathway. Methods: Male ICR mice were randomly divided into five groups: control, model, low- (1.5 × 106 CFU/mouse), medium- (1.5 × 107 CFU/mouse), and high- (1.5 × 108 CFU/mouse) dose L. rhamnosus Glory LG12. After 15 continuous days of gavage, all mice were further gavaged with loperamide hydrochloride suspension (4 mg/kg) to establish a mouse model of constipation. Body mass, the time taken until the first black stool excretion, the number and mass of black stool within 5 hours, small intestinal transit rate, serum levels of gastrointestinal regulatory peptides and inflammatory cytokines, colonic SCF and C-kit transcription levels were measured, and colonic pathology was examined. Results: Compared with the model group, medium- and high-dose L. rhamnosus Glory LG12 significantly reduced the time taken until the first black stool excretion (P < 0.001), increased the number and mass of black stools within 5 h (P < 0.01 and P < 0.001, respectively), and improved the ink propulsion rate in the small intestine (P < 0.001). In addition, both treatments elevated the serum levels of motilin (MTL), gastrin (Gas), substance P (SP), and interleukin 10 (IL-10), reduced the levels of endothelin-1 (ET-1), somatostatin (SS), vasoactive intestinal peptide (VIP), and IL-6, up-regulated the relative mRNA expression of SCF and C-kit in colonic tissue was (P < 0.01, P < 0.001), and restored damaged colonic barrier. Conclusion: L. rhamnosus Glory LG12 promotes gastrointestinal motility by affecting the release of gastrointestinal regulatory peptides and inflammatory cytokines through the modulation of the SCF/C-kit signaling pathway, thereby alleviating constipation symptoms. |
| format | Article |
| id | doaj-art-beb6adb456654ad5985f42682875dbd7 |
| institution | Kabale University |
| issn | 1002-6630 |
| language | English |
| publishDate | 2025-02-01 |
| publisher | China Food Publishing Company |
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| series | Shipin Kexue |
| spelling | doaj-art-beb6adb456654ad5985f42682875dbd72025-02-05T09:08:33ZengChina Food Publishing CompanyShipin Kexue1002-66302025-02-01463202610.7506/spkx1002-6630-20240710-109Laxative Effect of Lacticaseibacillus rhamnosus Glory LG12: from the Perspective of the Stem Cell Factor/C-kit Signaling PathwayXIANG Fangqin, LI Yuxing, LI Bailiang0(College of Food Science, Northeast Agricultural University, Harbin 150006, China)Purpose: To explore the laxative effect of Lacticaseibacillus rhamnosus Glory LG12 from the perspective of the stem cell factor (SCF)/C-kit signaling pathway. Methods: Male ICR mice were randomly divided into five groups: control, model, low- (1.5 × 106 CFU/mouse), medium- (1.5 × 107 CFU/mouse), and high- (1.5 × 108 CFU/mouse) dose L. rhamnosus Glory LG12. After 15 continuous days of gavage, all mice were further gavaged with loperamide hydrochloride suspension (4 mg/kg) to establish a mouse model of constipation. Body mass, the time taken until the first black stool excretion, the number and mass of black stool within 5 hours, small intestinal transit rate, serum levels of gastrointestinal regulatory peptides and inflammatory cytokines, colonic SCF and C-kit transcription levels were measured, and colonic pathology was examined. Results: Compared with the model group, medium- and high-dose L. rhamnosus Glory LG12 significantly reduced the time taken until the first black stool excretion (P < 0.001), increased the number and mass of black stools within 5 h (P < 0.01 and P < 0.001, respectively), and improved the ink propulsion rate in the small intestine (P < 0.001). In addition, both treatments elevated the serum levels of motilin (MTL), gastrin (Gas), substance P (SP), and interleukin 10 (IL-10), reduced the levels of endothelin-1 (ET-1), somatostatin (SS), vasoactive intestinal peptide (VIP), and IL-6, up-regulated the relative mRNA expression of SCF and C-kit in colonic tissue was (P < 0.01, P < 0.001), and restored damaged colonic barrier. Conclusion: L. rhamnosus Glory LG12 promotes gastrointestinal motility by affecting the release of gastrointestinal regulatory peptides and inflammatory cytokines through the modulation of the SCF/C-kit signaling pathway, thereby alleviating constipation symptoms.https://www.spkx.net.cn/fileup/1002-6630/PDF/2025-46-3-003.pdflacticaseibacillus rhamnosus glory lg12; constipation; laxative effect; inflammatory cytokines; gastrointestinal regulatory peptides; stem cell factor/c-kit signaling pathway |
| spellingShingle | XIANG Fangqin, LI Yuxing, LI Bailiang Laxative Effect of Lacticaseibacillus rhamnosus Glory LG12: from the Perspective of the Stem Cell Factor/C-kit Signaling Pathway Shipin Kexue lacticaseibacillus rhamnosus glory lg12; constipation; laxative effect; inflammatory cytokines; gastrointestinal regulatory peptides; stem cell factor/c-kit signaling pathway |
| title | Laxative Effect of Lacticaseibacillus rhamnosus Glory LG12: from the Perspective of the Stem Cell Factor/C-kit Signaling Pathway |
| title_full | Laxative Effect of Lacticaseibacillus rhamnosus Glory LG12: from the Perspective of the Stem Cell Factor/C-kit Signaling Pathway |
| title_fullStr | Laxative Effect of Lacticaseibacillus rhamnosus Glory LG12: from the Perspective of the Stem Cell Factor/C-kit Signaling Pathway |
| title_full_unstemmed | Laxative Effect of Lacticaseibacillus rhamnosus Glory LG12: from the Perspective of the Stem Cell Factor/C-kit Signaling Pathway |
| title_short | Laxative Effect of Lacticaseibacillus rhamnosus Glory LG12: from the Perspective of the Stem Cell Factor/C-kit Signaling Pathway |
| title_sort | laxative effect of lacticaseibacillus rhamnosus glory lg12 from the perspective of the stem cell factor c kit signaling pathway |
| topic | lacticaseibacillus rhamnosus glory lg12; constipation; laxative effect; inflammatory cytokines; gastrointestinal regulatory peptides; stem cell factor/c-kit signaling pathway |
| url | https://www.spkx.net.cn/fileup/1002-6630/PDF/2025-46-3-003.pdf |
| work_keys_str_mv | AT xiangfangqinliyuxinglibailiang laxativeeffectoflacticaseibacillusrhamnosusglorylg12fromtheperspectiveofthestemcellfactorckitsignalingpathway |