Antioxidant and anti-inflammatory activity of capitula, leaf and stem extracts of Tanacetum cilicicum (Boiss.) Grierson
In this study, various extracts obtained different parts of Tanacetum cilicicum were investigated for in vitro antioxidant and anti-inflammatory activity. Antioxidant activity was tested with three methods; namely 2.2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging capacity, 2.2′‐azinobis (3‐eth...
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| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
| Published: |
izzet kara
2019-07-01
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| Series: | International Journal of Secondary Metabolite |
| Subjects: | |
| Online Access: | https://dergipark.org.tr/tr/pub/ijsm/issue/44217/510316?publisher=ijate |
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| Summary: | In
this study, various extracts obtained different parts of Tanacetum cilicicum were investigated for in vitro antioxidant and anti-inflammatory activity. Antioxidant
activity was tested with three methods; namely 2.2-diphenyl-1-picrylhydrazyl
(DPPH) radical scavenging capacity, 2.2′‐azinobis
(3‐ethylbenzothiazolin‐6‐sulphonic acid) (ABTS) radical cation scavenging
capacity, and ferric-reducing antioxidant power (FRAP) assays. Total phenolic
and flavonoid contents of extracts were determined by Folin-Ciocalteu and
aluminum chloride methods, respectively. Also, anti-inflammatory activity of
these extracts was evaluated by 5-lipoxygenase inhibition assay. Ethyl acetate
extract of capitula of T. cilicicum
(TCCEA) showed the highest antioxidant activity with IC50 values of
22.44 and 30.86 µg/mL against DPPH and ABTS radicals, respectively. At the same
time, the highest ferric reducing power was found in the TCCEA (42.2 mg TE/g
extract). The highest total phenolic contents have been detected in TCCEA and
ethyl acetate extract of leaves of T.
cilicicum (TCLEA) with value of 174.1 and 175.6 mg GAE/g extract,
respectively. Similarly, the highest total flavonoid contents have been
detected in TCCEA and TCLEA with values of 26.94 and 30.48 mg QE/g extract,
respectively. TCCEA exhibited strong anti-inflammatory activity with IC50
value of 9.44 µg/mL when compared to standard indomethacine (22.39 µg/mL).
These results demonstrate that TCCEA has a significant antioxidant and
anti-inflammatory activity. Also, the results show that TCCEA is a good
candidate for further bioactivity-guided fractionation in the search for new
active anti-inflammatory and antioxidant compounds. |
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| ISSN: | 2148-6905 |