Protocol for the quantitative detection of mtDNA in the supernatants of activated human naive CD8+ T cells
Summary: Here, we present a protocol for the quantitative characterization of human T cell aging. We describe steps for sample collection; peripheral blood mononuclear cell (PBMC) isolation; and the enrichment, assessment, and activation of naive CD8+ T cells. We then detail procedures for supernata...
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| Language: | English |
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Elsevier
2025-03-01
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| Series: | STAR Protocols |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2666166725000188 |
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| author | Linlin Li Jun Jin |
| author_facet | Linlin Li Jun Jin |
| author_sort | Linlin Li |
| collection | DOAJ |
| description | Summary: Here, we present a protocol for the quantitative characterization of human T cell aging. We describe steps for sample collection; peripheral blood mononuclear cell (PBMC) isolation; and the enrichment, assessment, and activation of naive CD8+ T cells. We then detail procedures for supernatant collection and quantification using the absolute copy number of mitochondrial DNA (mtDNA) released into the supernatants from in-vitro-activated naive CD8+ T cells. We also apply this approach to predict the occurrence of lung adenocarcinoma in middle-aged populations.For complete details on the use and execution of this protocol, please refer to Jin et al.1 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics. |
| format | Article |
| id | doaj-art-beaaf7da3516444fba64203ed2b05bf9 |
| institution | Kabale University |
| issn | 2666-1667 |
| language | English |
| publishDate | 2025-03-01 |
| publisher | Elsevier |
| record_format | Article |
| series | STAR Protocols |
| spelling | doaj-art-beaaf7da3516444fba64203ed2b05bf92025-02-02T05:29:21ZengElsevierSTAR Protocols2666-16672025-03-0161103612Protocol for the quantitative detection of mtDNA in the supernatants of activated human naive CD8+ T cellsLinlin Li0Jun Jin1Multiscale Research Institute for Complex Systems, Fudan University, Shanghai 200433, ChinaMultiscale Research Institute for Complex Systems, Fudan University, Shanghai 200433, China; Corresponding authorSummary: Here, we present a protocol for the quantitative characterization of human T cell aging. We describe steps for sample collection; peripheral blood mononuclear cell (PBMC) isolation; and the enrichment, assessment, and activation of naive CD8+ T cells. We then detail procedures for supernatant collection and quantification using the absolute copy number of mitochondrial DNA (mtDNA) released into the supernatants from in-vitro-activated naive CD8+ T cells. We also apply this approach to predict the occurrence of lung adenocarcinoma in middle-aged populations.For complete details on the use and execution of this protocol, please refer to Jin et al.1 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.http://www.sciencedirect.com/science/article/pii/S2666166725000188cell isolationhealth sciencesclinical protocolimmunologymetabolismmolecular biology |
| spellingShingle | Linlin Li Jun Jin Protocol for the quantitative detection of mtDNA in the supernatants of activated human naive CD8+ T cells STAR Protocols cell isolation health sciences clinical protocol immunology metabolism molecular biology |
| title | Protocol for the quantitative detection of mtDNA in the supernatants of activated human naive CD8+ T cells |
| title_full | Protocol for the quantitative detection of mtDNA in the supernatants of activated human naive CD8+ T cells |
| title_fullStr | Protocol for the quantitative detection of mtDNA in the supernatants of activated human naive CD8+ T cells |
| title_full_unstemmed | Protocol for the quantitative detection of mtDNA in the supernatants of activated human naive CD8+ T cells |
| title_short | Protocol for the quantitative detection of mtDNA in the supernatants of activated human naive CD8+ T cells |
| title_sort | protocol for the quantitative detection of mtdna in the supernatants of activated human naive cd8 t cells |
| topic | cell isolation health sciences clinical protocol immunology metabolism molecular biology |
| url | http://www.sciencedirect.com/science/article/pii/S2666166725000188 |
| work_keys_str_mv | AT linlinli protocolforthequantitativedetectionofmtdnainthesupernatantsofactivatedhumannaivecd8tcells AT junjin protocolforthequantitativedetectionofmtdnainthesupernatantsofactivatedhumannaivecd8tcells |