Effects of defined voluntary running distances coupled with high‐fat diet consumption on the skeletal muscle transcriptome of male mice
Abstract Exercise counters many adverse health effects of consuming a high‐fat diet (HFD). However, complex molecular changes that occur in skeletal muscle in response to exercising while consuming a HFD are not yet known. We investigated the interplay between diverse exercise regimes and HFD consum...
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Wiley
2025-01-01
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| Series: | Physiological Reports |
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| Online Access: | https://doi.org/10.14814/phy2.70170 |
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| author | Afrina Brishti Sarah J. Johnson Daniel G. Palmer Md Obayed Raihan Lin Yan Shanon L. Casperson |
| author_facet | Afrina Brishti Sarah J. Johnson Daniel G. Palmer Md Obayed Raihan Lin Yan Shanon L. Casperson |
| author_sort | Afrina Brishti |
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| description | Abstract Exercise counters many adverse health effects of consuming a high‐fat diet (HFD). However, complex molecular changes that occur in skeletal muscle in response to exercising while consuming a HFD are not yet known. We investigated the interplay between diverse exercise regimes and HFD consumption on the adaptation of skeletal muscle transcriptome. C57BL/6 male mice were randomized into five groups—one sedentary control group and four exercise groups. The exercise groups consisted of an unrestricted running group (8.3 km/day) and three groups that were restricted to 75%, 50%, or 25% of unrestricted running (6.3, 4.2, and 2.1 km/day, respectively). Total RNA was extracted from frozen gastrocnemius muscle for transcriptome analyses. DEG counts were 1347, 1823, 1103, and 1107 and there were 107, 169, 67, and 89 unique genes present in the HFD‐25%, HFD‐50%, HFD‐75%, and HFD‐U, respectively. Comparing exercise groups, we found that exercising at 50% resulted in the most differentially expressed transcripts with the MAPK and PPAR signaling pathways enriched in down‐ and up‐regulated genes, respectively. These results demonstrate that running distance impacts the adaptation of the skeletal muscle transcriptome to exercise and suggest that middle‐distance running may provide the greatest protection against high‐fat diet‐induced stress coupled with exercise. |
| format | Article |
| id | doaj-art-bea0f89ce5444bfe99d39cdddde70629 |
| institution | Kabale University |
| issn | 2051-817X |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Wiley |
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| series | Physiological Reports |
| spelling | doaj-art-bea0f89ce5444bfe99d39cdddde706292025-01-27T13:49:52ZengWileyPhysiological Reports2051-817X2025-01-01132n/an/a10.14814/phy2.70170Effects of defined voluntary running distances coupled with high‐fat diet consumption on the skeletal muscle transcriptome of male miceAfrina Brishti0Sarah J. Johnson1Daniel G. Palmer2Md Obayed Raihan3Lin Yan4Shanon L. Casperson5United States Department of Agriculture, Agricultural Research Service Grand Forks Human Nutrition Research Center Grand Forks North Dakota USAUnited States Department of Agriculture, Agricultural Research Service Grand Forks Human Nutrition Research Center Grand Forks North Dakota USAUnited States Department of Agriculture, Agricultural Research Service Grand Forks Human Nutrition Research Center Grand Forks North Dakota USADepartment of Pharmaceutical Sciences, College of Health Sciences and Pharmacy Chicago State University Chicago Illinois USAUnited States Department of Agriculture, Agricultural Research Service Grand Forks Human Nutrition Research Center Grand Forks North Dakota USAUnited States Department of Agriculture, Agricultural Research Service Grand Forks Human Nutrition Research Center Grand Forks North Dakota USAAbstract Exercise counters many adverse health effects of consuming a high‐fat diet (HFD). However, complex molecular changes that occur in skeletal muscle in response to exercising while consuming a HFD are not yet known. We investigated the interplay between diverse exercise regimes and HFD consumption on the adaptation of skeletal muscle transcriptome. C57BL/6 male mice were randomized into five groups—one sedentary control group and four exercise groups. The exercise groups consisted of an unrestricted running group (8.3 km/day) and three groups that were restricted to 75%, 50%, or 25% of unrestricted running (6.3, 4.2, and 2.1 km/day, respectively). Total RNA was extracted from frozen gastrocnemius muscle for transcriptome analyses. DEG counts were 1347, 1823, 1103, and 1107 and there were 107, 169, 67, and 89 unique genes present in the HFD‐25%, HFD‐50%, HFD‐75%, and HFD‐U, respectively. Comparing exercise groups, we found that exercising at 50% resulted in the most differentially expressed transcripts with the MAPK and PPAR signaling pathways enriched in down‐ and up‐regulated genes, respectively. These results demonstrate that running distance impacts the adaptation of the skeletal muscle transcriptome to exercise and suggest that middle‐distance running may provide the greatest protection against high‐fat diet‐induced stress coupled with exercise.https://doi.org/10.14814/phy2.70170high‐fat dietskeletal muscletranscriptomevoluntary exercise |
| spellingShingle | Afrina Brishti Sarah J. Johnson Daniel G. Palmer Md Obayed Raihan Lin Yan Shanon L. Casperson Effects of defined voluntary running distances coupled with high‐fat diet consumption on the skeletal muscle transcriptome of male mice Physiological Reports high‐fat diet skeletal muscle transcriptome voluntary exercise |
| title | Effects of defined voluntary running distances coupled with high‐fat diet consumption on the skeletal muscle transcriptome of male mice |
| title_full | Effects of defined voluntary running distances coupled with high‐fat diet consumption on the skeletal muscle transcriptome of male mice |
| title_fullStr | Effects of defined voluntary running distances coupled with high‐fat diet consumption on the skeletal muscle transcriptome of male mice |
| title_full_unstemmed | Effects of defined voluntary running distances coupled with high‐fat diet consumption on the skeletal muscle transcriptome of male mice |
| title_short | Effects of defined voluntary running distances coupled with high‐fat diet consumption on the skeletal muscle transcriptome of male mice |
| title_sort | effects of defined voluntary running distances coupled with high fat diet consumption on the skeletal muscle transcriptome of male mice |
| topic | high‐fat diet skeletal muscle transcriptome voluntary exercise |
| url | https://doi.org/10.14814/phy2.70170 |
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