Regulation of histone H4 acetylation in the CNS and defensive behavior command neurons of the mollusk Helix mediated by serotonin and neuropeptide FMRFamide
Epigenetic mechanisms are commonly known to underlie memory formation. Presently, scientists’ attention is focused on changes in the levels of histone modifications (mainly acetylation and methylation) in the chromatin of CNS cells tested in various experimental models. Owing to their relatively sim...
Saved in:
| Main Authors: | , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Siberian Branch of the Russian Academy of Sciences, Federal Research Center Institute of Cytology and Genetics, The Vavilov Society of Geneticists and Breeders
2018-08-01
|
| Series: | Вавиловский журнал генетики и селекции |
| Subjects: | |
| Online Access: | https://vavilov.elpub.ru/jour/article/view/1597 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832575183582396416 |
|---|---|
| author | L. N. Grinkevich T. G. Zachepilo |
| author_facet | L. N. Grinkevich T. G. Zachepilo |
| author_sort | L. N. Grinkevich |
| collection | DOAJ |
| description | Epigenetic mechanisms are commonly known to underlie memory formation. Presently, scientists’ attention is focused on changes in the levels of histone modifications (mainly acetylation and methylation) in the chromatin of CNS cells tested in various experimental models. Owing to their relatively simple CNSs, mollusks are among the most popular models. Our experiments were con-ducted with the mollusk Helix lucorum because its CNS had been investigated in detail and most of its neurons had been proven to participate in the formation of different behavior patterns, including the prolonged response to various stimuli. This work concerns the influence of various effectors (serotonin and FMRFamide, associated with CNS activator and inhibitory pathways, respectively) on the acetylation of H4 histone in the subesophageal ganglion complex and in defensive behavior command neurons of the right and left parietal ganglia (RPa3/2 and LPa3/2) in the snail. Western blot analysis showed that FMRFamide inhibited histone H4 acetylation induced by serotonin in the subesophageal complex of CNS ganglia. However, serotonin and FMRFamide cooperatively enhanced the induction of histone H4 acetylation in RPa3/2 defensive behavior command neurons. No changes were found in the counterpart LPa3/2. It is a new piece of evidence for functional asymmetry in Helix. The inhibitory pathways mediated by FMRFamide not only inhibit the activatory intracellular processes in the entire CNS but can also enhance them, as in RPa3/2 defensive behavior command neurons. |
| format | Article |
| id | doaj-art-be963913030a4fe78b3293313749e370 |
| institution | Kabale University |
| issn | 2500-3259 |
| language | English |
| publishDate | 2018-08-01 |
| publisher | Siberian Branch of the Russian Academy of Sciences, Federal Research Center Institute of Cytology and Genetics, The Vavilov Society of Geneticists and Breeders |
| record_format | Article |
| series | Вавиловский журнал генетики и селекции |
| spelling | doaj-art-be963913030a4fe78b3293313749e3702025-02-01T09:58:06ZengSiberian Branch of the Russian Academy of Sciences, Federal Research Center Institute of Cytology and Genetics, The Vavilov Society of Geneticists and BreedersВавиловский журнал генетики и селекции2500-32592018-08-0122560661010.18699/VJ18.401799Regulation of histone H4 acetylation in the CNS and defensive behavior command neurons of the mollusk Helix mediated by serotonin and neuropeptide FMRFamideL. N. Grinkevich0T. G. Zachepilo1Pavlov Institute of Physiology, RASPavlov Institute of Physiology, RASEpigenetic mechanisms are commonly known to underlie memory formation. Presently, scientists’ attention is focused on changes in the levels of histone modifications (mainly acetylation and methylation) in the chromatin of CNS cells tested in various experimental models. Owing to their relatively simple CNSs, mollusks are among the most popular models. Our experiments were con-ducted with the mollusk Helix lucorum because its CNS had been investigated in detail and most of its neurons had been proven to participate in the formation of different behavior patterns, including the prolonged response to various stimuli. This work concerns the influence of various effectors (serotonin and FMRFamide, associated with CNS activator and inhibitory pathways, respectively) on the acetylation of H4 histone in the subesophageal ganglion complex and in defensive behavior command neurons of the right and left parietal ganglia (RPa3/2 and LPa3/2) in the snail. Western blot analysis showed that FMRFamide inhibited histone H4 acetylation induced by serotonin in the subesophageal complex of CNS ganglia. However, serotonin and FMRFamide cooperatively enhanced the induction of histone H4 acetylation in RPa3/2 defensive behavior command neurons. No changes were found in the counterpart LPa3/2. It is a new piece of evidence for functional asymmetry in Helix. The inhibitory pathways mediated by FMRFamide not only inhibit the activatory intracellular processes in the entire CNS but can also enhance them, as in RPa3/2 defensive behavior command neurons.https://vavilov.elpub.ru/jour/article/view/1597mollusk helixwestern blottingepigeneticslong-term memoryhistone h4 acetylationserotoninfmrfamidecommand neurons |
| spellingShingle | L. N. Grinkevich T. G. Zachepilo Regulation of histone H4 acetylation in the CNS and defensive behavior command neurons of the mollusk Helix mediated by serotonin and neuropeptide FMRFamide Вавиловский журнал генетики и селекции mollusk helix western blotting epigenetics long-term memory histone h4 acetylation serotonin fmrfamide command neurons |
| title | Regulation of histone H4 acetylation in the CNS and defensive behavior command neurons of the mollusk Helix mediated by serotonin and neuropeptide FMRFamide |
| title_full | Regulation of histone H4 acetylation in the CNS and defensive behavior command neurons of the mollusk Helix mediated by serotonin and neuropeptide FMRFamide |
| title_fullStr | Regulation of histone H4 acetylation in the CNS and defensive behavior command neurons of the mollusk Helix mediated by serotonin and neuropeptide FMRFamide |
| title_full_unstemmed | Regulation of histone H4 acetylation in the CNS and defensive behavior command neurons of the mollusk Helix mediated by serotonin and neuropeptide FMRFamide |
| title_short | Regulation of histone H4 acetylation in the CNS and defensive behavior command neurons of the mollusk Helix mediated by serotonin and neuropeptide FMRFamide |
| title_sort | regulation of histone h4 acetylation in the cns and defensive behavior command neurons of the mollusk helix mediated by serotonin and neuropeptide fmrfamide |
| topic | mollusk helix western blotting epigenetics long-term memory histone h4 acetylation serotonin fmrfamide command neurons |
| url | https://vavilov.elpub.ru/jour/article/view/1597 |
| work_keys_str_mv | AT lngrinkevich regulationofhistoneh4acetylationinthecnsanddefensivebehaviorcommandneuronsofthemolluskhelixmediatedbyserotoninandneuropeptidefmrfamide AT tgzachepilo regulationofhistoneh4acetylationinthecnsanddefensivebehaviorcommandneuronsofthemolluskhelixmediatedbyserotoninandneuropeptidefmrfamide |