Enhanced Engraftment of a Very Low-Dose Cord Blood Unit in an Adult Haemopoietic Transplant by Addition of Six Mismatched Viable Cord Units
The report describes the feasibility of the addition of multiple viable HLA-mismatched unrelated cord blood units, to a low cell number matched unrelated cord, to assist clinical engraftment. An ablative stem cell transplant was performed in an adult with relapsed acute lymphoblastic leukaemia (ALL)...
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| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2010-01-01
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| Series: | Stem Cells International |
| Online Access: | http://dx.doi.org/10.4061/2010/431909 |
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| author | Stephen J. Proctor Catherine E. Chapman Rachel Sharples Helen L. Lucraft Jennifer Wilkinson Jane Conn Peter G. Middleton |
| author_facet | Stephen J. Proctor Catherine E. Chapman Rachel Sharples Helen L. Lucraft Jennifer Wilkinson Jane Conn Peter G. Middleton |
| author_sort | Stephen J. Proctor |
| collection | DOAJ |
| description | The report describes the feasibility of the addition of multiple viable HLA-mismatched unrelated cord blood units, to a low cell number matched unrelated cord, to assist clinical engraftment. An ablative stem cell transplant was performed in an adult with relapsed acute lymphoblastic leukaemia (ALL), using a single HLA-matched cord blood unit (mononuclear cell dose 0.8×107
), supported by six mismatched cord blood units (one unit per 10 kg recipient weight). No adverse reaction occurred following the infusion of mismatched units and engraftment of the suboptimal-dose matched unit occurred rapidly, with no molecular evidence of engraftment of mismatched cords. Early molecular remission of ALL was demonstrated using a novel PCR for a mitochondrial DNA mutation in the leukaemic clone. The cell dose of the matched cord was well below that recommended to engraft a 70 kg recipient. We suggest that a factor or factors in the mismatched cords enhanced/supported engraftment of the matched cord. |
| format | Article |
| id | doaj-art-be763b9318774d4e8b580fecc16ad6bf |
| institution | Kabale University |
| issn | 1687-9678 |
| language | English |
| publishDate | 2010-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Stem Cells International |
| spelling | doaj-art-be763b9318774d4e8b580fecc16ad6bf2025-02-03T01:08:54ZengWileyStem Cells International1687-96782010-01-01201010.4061/2010/431909431909Enhanced Engraftment of a Very Low-Dose Cord Blood Unit in an Adult Haemopoietic Transplant by Addition of Six Mismatched Viable Cord UnitsStephen J. Proctor0Catherine E. Chapman1Rachel Sharples2Helen L. Lucraft3Jennifer Wilkinson4Jane Conn5Peter G. Middleton6Haematological oncology Group, Academic Haematology, Medical School, Newcastle University, Newcastle upon Tyne NE2 4HH, UKMedical Department, Newcastle Blood Centre, NHS Blood and Transplant, Holland Drive, Newcastle upon Tyne NE2 4NQ, UKNewcastle NHS Hospital Foundation Trust, Freeman Road, Newcastle upon Tyne NE7 7DN, UKNorthern Centre for Cancer Treatment, Newcastle NHS Hospital Foundation Trust, Freeman Road, Newcastle upon Tyne, NE7 7DN, UKHaematological oncology Group, Academic Haematology, Medical School, Newcastle University, Newcastle upon Tyne NE2 4HH, UKNewcastle NHS Hospital Foundation Trust, Freeman Road, Newcastle upon Tyne NE7 7DN, UKHaematological oncology Group, Academic Haematology, Medical School, Newcastle University, Newcastle upon Tyne NE2 4HH, UKThe report describes the feasibility of the addition of multiple viable HLA-mismatched unrelated cord blood units, to a low cell number matched unrelated cord, to assist clinical engraftment. An ablative stem cell transplant was performed in an adult with relapsed acute lymphoblastic leukaemia (ALL), using a single HLA-matched cord blood unit (mononuclear cell dose 0.8×107 ), supported by six mismatched cord blood units (one unit per 10 kg recipient weight). No adverse reaction occurred following the infusion of mismatched units and engraftment of the suboptimal-dose matched unit occurred rapidly, with no molecular evidence of engraftment of mismatched cords. Early molecular remission of ALL was demonstrated using a novel PCR for a mitochondrial DNA mutation in the leukaemic clone. The cell dose of the matched cord was well below that recommended to engraft a 70 kg recipient. We suggest that a factor or factors in the mismatched cords enhanced/supported engraftment of the matched cord.http://dx.doi.org/10.4061/2010/431909 |
| spellingShingle | Stephen J. Proctor Catherine E. Chapman Rachel Sharples Helen L. Lucraft Jennifer Wilkinson Jane Conn Peter G. Middleton Enhanced Engraftment of a Very Low-Dose Cord Blood Unit in an Adult Haemopoietic Transplant by Addition of Six Mismatched Viable Cord Units Stem Cells International |
| title | Enhanced Engraftment of a Very Low-Dose Cord Blood Unit in an Adult Haemopoietic Transplant by Addition of Six Mismatched Viable Cord Units |
| title_full | Enhanced Engraftment of a Very Low-Dose Cord Blood Unit in an Adult Haemopoietic Transplant by Addition of Six Mismatched Viable Cord Units |
| title_fullStr | Enhanced Engraftment of a Very Low-Dose Cord Blood Unit in an Adult Haemopoietic Transplant by Addition of Six Mismatched Viable Cord Units |
| title_full_unstemmed | Enhanced Engraftment of a Very Low-Dose Cord Blood Unit in an Adult Haemopoietic Transplant by Addition of Six Mismatched Viable Cord Units |
| title_short | Enhanced Engraftment of a Very Low-Dose Cord Blood Unit in an Adult Haemopoietic Transplant by Addition of Six Mismatched Viable Cord Units |
| title_sort | enhanced engraftment of a very low dose cord blood unit in an adult haemopoietic transplant by addition of six mismatched viable cord units |
| url | http://dx.doi.org/10.4061/2010/431909 |
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