Salivary stimulatory and anti-inflammatory effects of aqueous Lilium brownii var. viridulum Baker extract for Sjögren’s syndrome: in vitro and in vivo studies
Abstract Sjögren’s syndrome (SS) damages exocrine glands, and Lilium brownii var. viridulum Baker (Lilii Bulbus, LB) shows potential as a therapeutic agent. This study evaluated LB’s efficacy in alleviating xerostomia using non-obese diabetic/severe combined immunodeficiency (NOD/SCID) mice and huma...
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Universidade de São Paulo
2025-01-01
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| Series: | Brazilian Journal of Pharmaceutical Sciences |
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| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502025000100330&lng=en&tlng=en |
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| author | Na-Yeon Ha Haein Jeong Mun Seog Chang Jinsung Kim |
| author_facet | Na-Yeon Ha Haein Jeong Mun Seog Chang Jinsung Kim |
| author_sort | Na-Yeon Ha |
| collection | DOAJ |
| description | Abstract Sjögren’s syndrome (SS) damages exocrine glands, and Lilium brownii var. viridulum Baker (Lilii Bulbus, LB) shows potential as a therapeutic agent. This study evaluated LB’s efficacy in alleviating xerostomia using non-obese diabetic/severe combined immunodeficiency (NOD/SCID) mice and human salivary gland acinar (NS-SV-AC) cells. In vitro, NS-SV-AC cells were treated with LB (1, 5, 10, 50, and 100 µg/mL) and 5-aza-2′-deoxycytidine (2 µM/mL) for 48 h. Cell viability, fluid secretion, and aquaporin-5 (AQP-5) expression were assessed. In vivo, thirty 20-week-old NOD/SCID mice received LB orally (100, 500, and 1,000 mg/kg) for 4 weeks, with salivary secretion rates measured. AQP-5 and M3 muscarinic acetylcholine receptor (M3R) expression and inflammatory mediator levels were determined using western blotting and enzyme-linked immunosorbent assay. Histopathological examination of salivary glands was also performed. LB significantly increased NS-SV-AC cell proliferation, fluid secretion, and AQP-5 expression. In NOD/SCID mice, LB reduced anti-SSA/Ro and anti-SSB/La antibodies, tumor necrosis factor-α, interferon-γ, and interleukin-6, while increasing AQP-5 and M3R expression. This resulted in increased salivary secretion and reduced glandular inflammation. LB extract appears promising for managing oral health by enhancing salivation, upregulating AQP-5, and modulating immune-inflammatory responses. |
| format | Article |
| id | doaj-art-be62870bf48947e29c94f3fe68aaaf85 |
| institution | Kabale University |
| issn | 2175-9790 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Universidade de São Paulo |
| record_format | Article |
| series | Brazilian Journal of Pharmaceutical Sciences |
| spelling | doaj-art-be62870bf48947e29c94f3fe68aaaf852025-01-21T07:41:13ZengUniversidade de São PauloBrazilian Journal of Pharmaceutical Sciences2175-97902025-01-016110.1590/s2175-97902025e24311Salivary stimulatory and anti-inflammatory effects of aqueous Lilium brownii var. viridulum Baker extract for Sjögren’s syndrome: in vitro and in vivo studiesNa-Yeon HaHaein JeongMun Seog ChangJinsung Kimhttps://orcid.org/0000-0002-2164-357XAbstract Sjögren’s syndrome (SS) damages exocrine glands, and Lilium brownii var. viridulum Baker (Lilii Bulbus, LB) shows potential as a therapeutic agent. This study evaluated LB’s efficacy in alleviating xerostomia using non-obese diabetic/severe combined immunodeficiency (NOD/SCID) mice and human salivary gland acinar (NS-SV-AC) cells. In vitro, NS-SV-AC cells were treated with LB (1, 5, 10, 50, and 100 µg/mL) and 5-aza-2′-deoxycytidine (2 µM/mL) for 48 h. Cell viability, fluid secretion, and aquaporin-5 (AQP-5) expression were assessed. In vivo, thirty 20-week-old NOD/SCID mice received LB orally (100, 500, and 1,000 mg/kg) for 4 weeks, with salivary secretion rates measured. AQP-5 and M3 muscarinic acetylcholine receptor (M3R) expression and inflammatory mediator levels were determined using western blotting and enzyme-linked immunosorbent assay. Histopathological examination of salivary glands was also performed. LB significantly increased NS-SV-AC cell proliferation, fluid secretion, and AQP-5 expression. In NOD/SCID mice, LB reduced anti-SSA/Ro and anti-SSB/La antibodies, tumor necrosis factor-α, interferon-γ, and interleukin-6, while increasing AQP-5 and M3R expression. This resulted in increased salivary secretion and reduced glandular inflammation. LB extract appears promising for managing oral health by enhancing salivation, upregulating AQP-5, and modulating immune-inflammatory responses.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502025000100330&lng=en&tlng=enSjögren’s syndromeSalivaryDry mouthLiliiLilium |
| spellingShingle | Na-Yeon Ha Haein Jeong Mun Seog Chang Jinsung Kim Salivary stimulatory and anti-inflammatory effects of aqueous Lilium brownii var. viridulum Baker extract for Sjögren’s syndrome: in vitro and in vivo studies Brazilian Journal of Pharmaceutical Sciences Sjögren’s syndrome Salivary Dry mouth Lilii Lilium |
| title | Salivary stimulatory and anti-inflammatory effects of aqueous Lilium brownii var. viridulum Baker extract for Sjögren’s syndrome: in vitro and in vivo studies |
| title_full | Salivary stimulatory and anti-inflammatory effects of aqueous Lilium brownii var. viridulum Baker extract for Sjögren’s syndrome: in vitro and in vivo studies |
| title_fullStr | Salivary stimulatory and anti-inflammatory effects of aqueous Lilium brownii var. viridulum Baker extract for Sjögren’s syndrome: in vitro and in vivo studies |
| title_full_unstemmed | Salivary stimulatory and anti-inflammatory effects of aqueous Lilium brownii var. viridulum Baker extract for Sjögren’s syndrome: in vitro and in vivo studies |
| title_short | Salivary stimulatory and anti-inflammatory effects of aqueous Lilium brownii var. viridulum Baker extract for Sjögren’s syndrome: in vitro and in vivo studies |
| title_sort | salivary stimulatory and anti inflammatory effects of aqueous lilium brownii var viridulum baker extract for sjogren s syndrome in vitro and in vivo studies |
| topic | Sjögren’s syndrome Salivary Dry mouth Lilii Lilium |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502025000100330&lng=en&tlng=en |
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