Large-Scale Plantlet Conversion and Ex Vitro Transplantation Efficiency of Siberian Ginseng by Bioreactor Culture
To achieve large-scale low-cost ex vitro acclimatization of Siberian ginseng plants, heart- and torpedo-shaped secondary somatic embryos (SEs) induced from germinated SEs on agar medium were collected and then inoculated to 10-l bubble column bioreactor, respectively. For plantlet conversion, inocul...
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| Format: | Article |
| Language: | English |
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Wiley
2013-01-01
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| Series: | The Scientific World Journal |
| Online Access: | http://dx.doi.org/10.1155/2013/829067 |
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| author | Jingli Yang Shicheng Zhao Changyeon Yu Chenghao Li |
| author_facet | Jingli Yang Shicheng Zhao Changyeon Yu Chenghao Li |
| author_sort | Jingli Yang |
| collection | DOAJ |
| description | To achieve large-scale low-cost ex vitro acclimatization of Siberian ginseng plants, heart- and torpedo-shaped secondary somatic embryos (SEs) induced from germinated SEs on agar medium were collected and then inoculated to 10-l bubble column bioreactor, respectively. For plantlet conversion, inoculation of torpedo-shaped secondary SEs was more effective than heart-shaped SEs. TS2 (culture of torpedo-shaped SEs in a bioreactor with a 2-week subculture interval) plantlets had a higher root number and leaf number and larger leaf area than did HS3 (culture of heart-shaped SEs in a bioreactor with a 3-week subculture interval) and HS2 (culture of heart-shaped SEs in a bioreactor with a 2-week subculture interval) plantlets. Of these converted plants, TS2 plantlets had higher survival rate (83.7%) and growth characteristics after transplantation in a simple shed covered with a 50% sunshade net only for 6 months. TS2 plantlets also showed significantly lower H2O2 content and significantly increased superoxide dismutase (SOD), glutathione peroxidase (GPX), and glutathione transferase (GST) expression levels as compared to HS2 plants when exposure to ex vitro conditions. |
| format | Article |
| id | doaj-art-be5a75db4b2040a4ad24b8f2e291a651 |
| institution | Kabale University |
| issn | 1537-744X |
| language | English |
| publishDate | 2013-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | The Scientific World Journal |
| spelling | doaj-art-be5a75db4b2040a4ad24b8f2e291a6512025-02-03T01:26:18ZengWileyThe Scientific World Journal1537-744X2013-01-01201310.1155/2013/829067829067Large-Scale Plantlet Conversion and Ex Vitro Transplantation Efficiency of Siberian Ginseng by Bioreactor CultureJingli Yang0Shicheng Zhao1Changyeon Yu2Chenghao Li3State Key Laboratory of Tree Genetics and Breeding, Northeast Forestry University, Harbin 150040, ChinaDepartment of Crop Science, College of Agriculture & Life Sciences, Chungnam National University, Daejeon 305-764, Republic of KoreaDivision of Bioresource Technology, College of Agriculture and Life Sciences, Kangwon National University, Chuncheon 200-701, Republic of KoreaState Key Laboratory of Tree Genetics and Breeding, Northeast Forestry University, Harbin 150040, ChinaTo achieve large-scale low-cost ex vitro acclimatization of Siberian ginseng plants, heart- and torpedo-shaped secondary somatic embryos (SEs) induced from germinated SEs on agar medium were collected and then inoculated to 10-l bubble column bioreactor, respectively. For plantlet conversion, inoculation of torpedo-shaped secondary SEs was more effective than heart-shaped SEs. TS2 (culture of torpedo-shaped SEs in a bioreactor with a 2-week subculture interval) plantlets had a higher root number and leaf number and larger leaf area than did HS3 (culture of heart-shaped SEs in a bioreactor with a 3-week subculture interval) and HS2 (culture of heart-shaped SEs in a bioreactor with a 2-week subculture interval) plantlets. Of these converted plants, TS2 plantlets had higher survival rate (83.7%) and growth characteristics after transplantation in a simple shed covered with a 50% sunshade net only for 6 months. TS2 plantlets also showed significantly lower H2O2 content and significantly increased superoxide dismutase (SOD), glutathione peroxidase (GPX), and glutathione transferase (GST) expression levels as compared to HS2 plants when exposure to ex vitro conditions.http://dx.doi.org/10.1155/2013/829067 |
| spellingShingle | Jingli Yang Shicheng Zhao Changyeon Yu Chenghao Li Large-Scale Plantlet Conversion and Ex Vitro Transplantation Efficiency of Siberian Ginseng by Bioreactor Culture The Scientific World Journal |
| title | Large-Scale Plantlet Conversion and Ex Vitro Transplantation Efficiency of Siberian Ginseng by Bioreactor Culture |
| title_full | Large-Scale Plantlet Conversion and Ex Vitro Transplantation Efficiency of Siberian Ginseng by Bioreactor Culture |
| title_fullStr | Large-Scale Plantlet Conversion and Ex Vitro Transplantation Efficiency of Siberian Ginseng by Bioreactor Culture |
| title_full_unstemmed | Large-Scale Plantlet Conversion and Ex Vitro Transplantation Efficiency of Siberian Ginseng by Bioreactor Culture |
| title_short | Large-Scale Plantlet Conversion and Ex Vitro Transplantation Efficiency of Siberian Ginseng by Bioreactor Culture |
| title_sort | large scale plantlet conversion and ex vitro transplantation efficiency of siberian ginseng by bioreactor culture |
| url | http://dx.doi.org/10.1155/2013/829067 |
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