The mechanism of irisin via Nrf2/GPX4 signaling pathway in alleviating lipopolysaccha-ride-induced acute lung injury in rats
Objective To study the role of Nrf2/GPX4 signaling pathway in irisin alleviating lipopoly-saccharide (LPS)-induced acute lung injury in rats.Method Seventy-two healthy male SD rats were ran-domly divided into control group (C group), acute lung injury group (ALI group), irisin group (Ir group), iris...
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Editorial Office of New Medicine
2023-04-01
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| Series: | Yixue xinzhi zazhi |
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| Online Access: | https://yxxz.whuznhmedj.com/futureApi/storage/attach/2304/EkvwxSBPQ6aLusKc4JcZfymeGFK39L56RnUwqwM7.pdf |
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| author | Jing LUO Jun PENG Jing ZHANG Yun WANG Xin-Yi LI |
| author_facet | Jing LUO Jun PENG Jing ZHANG Yun WANG Xin-Yi LI |
| author_sort | Jing LUO |
| collection | DOAJ |
| description | Objective To study the role of Nrf2/GPX4 signaling pathway in irisin alleviating lipopoly-saccharide (LPS)-induced acute lung injury in rats.Method Seventy-two healthy male SD rats were ran-domly divided into control group (C group), acute lung injury group (ALI group), irisin group (Ir group), irisin+Nrf2 inhibitor group (Ir+ML385 group). 24 hours after the model preparation, bronchoalveolar lavage fluid (BALF) was collected for protein concentration determination, and the contents of IL-6 and TNF-α in BALF were detected by ELISA. Pathologic results of lung tissue sections were observed by HE staining and scored, and the wet/dry weight ratio of the lung tissue was calculated. The contents of Fe2+, MDA and GSH in lung tissue homogenate were detected by colorimetric method. The protein levels of GPX4, ACSL4 and nuclear Nrf2 in lung tissues were determined by western blot (WB).Result Compared with the control group, the protein contents in BALF, the IL-6 and TNF-α contents, lung pathological in-jury score, wet/dry weight ratio, the contents of Fe2+ and MDA were increased, the GSH content and GPX4 level were decreased, the protein levels of ACSL4 and nuclear Nrf2 were increased in ALI group (P |
| format | Article |
| id | doaj-art-be2b16032fcd48a3bb4854c6653e5da7 |
| institution | OA Journals |
| issn | 1004-5511 |
| language | zho |
| publishDate | 2023-04-01 |
| publisher | Editorial Office of New Medicine |
| record_format | Article |
| series | Yixue xinzhi zazhi |
| spelling | doaj-art-be2b16032fcd48a3bb4854c6653e5da72025-08-20T02:00:12ZzhoEditorial Office of New MedicineYixue xinzhi zazhi1004-55112023-04-01332839010.12173/j.issn.1004-5511.2022090466391The mechanism of irisin via Nrf2/GPX4 signaling pathway in alleviating lipopolysaccha-ride-induced acute lung injury in ratsJing LUOJun PENGJing ZHANGYun WANGXin-Yi LIObjective To study the role of Nrf2/GPX4 signaling pathway in irisin alleviating lipopoly-saccharide (LPS)-induced acute lung injury in rats.Method Seventy-two healthy male SD rats were ran-domly divided into control group (C group), acute lung injury group (ALI group), irisin group (Ir group), irisin+Nrf2 inhibitor group (Ir+ML385 group). 24 hours after the model preparation, bronchoalveolar lavage fluid (BALF) was collected for protein concentration determination, and the contents of IL-6 and TNF-α in BALF were detected by ELISA. Pathologic results of lung tissue sections were observed by HE staining and scored, and the wet/dry weight ratio of the lung tissue was calculated. The contents of Fe2+, MDA and GSH in lung tissue homogenate were detected by colorimetric method. The protein levels of GPX4, ACSL4 and nuclear Nrf2 in lung tissues were determined by western blot (WB).Result Compared with the control group, the protein contents in BALF, the IL-6 and TNF-α contents, lung pathological in-jury score, wet/dry weight ratio, the contents of Fe2+ and MDA were increased, the GSH content and GPX4 level were decreased, the protein levels of ACSL4 and nuclear Nrf2 were increased in ALI group (Phttps://yxxz.whuznhmedj.com/futureApi/storage/attach/2304/EkvwxSBPQ6aLusKc4JcZfymeGFK39L56RnUwqwM7.pdfirisinacute lung injurynrf2gpx4ferroptosis |
| spellingShingle | Jing LUO Jun PENG Jing ZHANG Yun WANG Xin-Yi LI The mechanism of irisin via Nrf2/GPX4 signaling pathway in alleviating lipopolysaccha-ride-induced acute lung injury in rats Yixue xinzhi zazhi irisin acute lung injury nrf2 gpx4 ferroptosis |
| title | The mechanism of irisin via Nrf2/GPX4 signaling pathway in alleviating lipopolysaccha-ride-induced acute lung injury in rats |
| title_full | The mechanism of irisin via Nrf2/GPX4 signaling pathway in alleviating lipopolysaccha-ride-induced acute lung injury in rats |
| title_fullStr | The mechanism of irisin via Nrf2/GPX4 signaling pathway in alleviating lipopolysaccha-ride-induced acute lung injury in rats |
| title_full_unstemmed | The mechanism of irisin via Nrf2/GPX4 signaling pathway in alleviating lipopolysaccha-ride-induced acute lung injury in rats |
| title_short | The mechanism of irisin via Nrf2/GPX4 signaling pathway in alleviating lipopolysaccha-ride-induced acute lung injury in rats |
| title_sort | mechanism of irisin via nrf2 gpx4 signaling pathway in alleviating lipopolysaccha ride induced acute lung injury in rats |
| topic | irisin acute lung injury nrf2 gpx4 ferroptosis |
| url | https://yxxz.whuznhmedj.com/futureApi/storage/attach/2304/EkvwxSBPQ6aLusKc4JcZfymeGFK39L56RnUwqwM7.pdf |
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