Genome-wide characterization and identification of root development and stress-related CsMYB36 genes

Cucumber (Cucumis sativus L.) is an important vegetable crop worldwide. Over the last two decades, there have been many breakthroughs in the understanding of various developmental processes in cucumber, such as shoot branching, sex differentiation, and leaf and fruit size and shape. Roots play an im...

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Main Authors: Chunhua Wang, Xi Shen, Ting Yang, Hongxin Yao, Xun Peng, Cheng Xiong, Hagai Cohen, Ning Hao, Jiajian Cao, Tao Wu
Format: Article
Language:English
Published: Maximum Academic Press 2023-01-01
Series:Vegetable Research
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Online Access:https://www.maxapress.com/article/doi/10.48130/VR-2023-0019
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author Chunhua Wang
Xi Shen
Ting Yang
Hongxin Yao
Xun Peng
Cheng Xiong
Hagai Cohen
Ning Hao
Jiajian Cao
Tao Wu
author_facet Chunhua Wang
Xi Shen
Ting Yang
Hongxin Yao
Xun Peng
Cheng Xiong
Hagai Cohen
Ning Hao
Jiajian Cao
Tao Wu
author_sort Chunhua Wang
collection DOAJ
description Cucumber (Cucumis sativus L.) is an important vegetable crop worldwide. Over the last two decades, there have been many breakthroughs in the understanding of various developmental processes in cucumber, such as shoot branching, sex differentiation, and leaf and fruit size and shape. Roots play an important role in plant growth and development and nutrient absorption, affect crop yield, and participate in the interaction between plants and abiotic stress signals. However, the discovery of essential genes in cucumber roots is very limited. MYB36 is a critical transcription factor for whole development that negatively regulates cell proliferation and thus orchestrates Casparian strip (CS) formation in the root endodermis. To identify CsMYB36 genes with functions in the CS of cucumber and their responses to stress, we described in detail the characteristics of the CsMYB36 gene family in cucumber in this study. A total of 15 CsMYB36 genes were found in the cucumber genome. Through phylogenetic and tissue-specific expression analysis, CsaV3_2G008030.1 and CsaV3_3G036040.1 were selected as candidates regulating CS formation. In addition, all CsMYB36s were found to have anaerobic induction elements, indicating that MYB36 is likely to be highly responsive to anaerobic environments, such as waterlogging. We also identified one potential candidate CsMYB36 (CsaV3_1G013560.1) that participates in high-temperature stress responses. This work provides valuable information for understanding the characteristics of the CsMYB36 gene family in cucumber and provides new clues for researchers to study high temperature, waterlogging stress and the formation of CS.
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spelling doaj-art-be21cb3fef5a49e7bee07afdcb6fe9c82025-08-20T02:12:16ZengMaximum Academic PressVegetable Research2769-05202023-01-013111110.48130/VR-2023-0019VR-2023-0019Genome-wide characterization and identification of root development and stress-related CsMYB36 genesChunhua Wang0Xi Shen1Ting Yang2Hongxin Yao3Xun Peng4Cheng Xiong5Hagai Cohen6Ning Hao7Jiajian Cao8Tao Wu9College of Horticulture, Hunan Agricultural University, Changsha 410128, ChinaCollege of Horticulture, Hunan Agricultural University, Changsha 410128, ChinaCollege of Horticulture, Hunan Agricultural University, Changsha 410128, ChinaCollege of Horticulture, Hunan Agricultural University, Changsha 410128, ChinaCollege of Horticulture, Hunan Agricultural University, Changsha 410128, ChinaCollege of Horticulture, Hunan Agricultural University, Changsha 410128, ChinaDepartment of Vegetable and Field Crops, Institute of Plant Science, Agricultural Research Organization (ARO), Volcani Center, Rishon Lezion 7505101, IsraelCollege of Horticulture, Hunan Agricultural University, Changsha 410128, ChinaCollege of Horticulture, Hunan Agricultural University, Changsha 410128, ChinaCollege of Horticulture, Hunan Agricultural University, Changsha 410128, ChinaCucumber (Cucumis sativus L.) is an important vegetable crop worldwide. Over the last two decades, there have been many breakthroughs in the understanding of various developmental processes in cucumber, such as shoot branching, sex differentiation, and leaf and fruit size and shape. Roots play an important role in plant growth and development and nutrient absorption, affect crop yield, and participate in the interaction between plants and abiotic stress signals. However, the discovery of essential genes in cucumber roots is very limited. MYB36 is a critical transcription factor for whole development that negatively regulates cell proliferation and thus orchestrates Casparian strip (CS) formation in the root endodermis. To identify CsMYB36 genes with functions in the CS of cucumber and their responses to stress, we described in detail the characteristics of the CsMYB36 gene family in cucumber in this study. A total of 15 CsMYB36 genes were found in the cucumber genome. Through phylogenetic and tissue-specific expression analysis, CsaV3_2G008030.1 and CsaV3_3G036040.1 were selected as candidates regulating CS formation. In addition, all CsMYB36s were found to have anaerobic induction elements, indicating that MYB36 is likely to be highly responsive to anaerobic environments, such as waterlogging. We also identified one potential candidate CsMYB36 (CsaV3_1G013560.1) that participates in high-temperature stress responses. This work provides valuable information for understanding the characteristics of the CsMYB36 gene family in cucumber and provides new clues for researchers to study high temperature, waterlogging stress and the formation of CS.https://www.maxapress.com/article/doi/10.48130/VR-2023-0019cucumbercsmyb36expression proflesgene family
spellingShingle Chunhua Wang
Xi Shen
Ting Yang
Hongxin Yao
Xun Peng
Cheng Xiong
Hagai Cohen
Ning Hao
Jiajian Cao
Tao Wu
Genome-wide characterization and identification of root development and stress-related CsMYB36 genes
Vegetable Research
cucumber
csmyb36
expression profles
gene family
title Genome-wide characterization and identification of root development and stress-related CsMYB36 genes
title_full Genome-wide characterization and identification of root development and stress-related CsMYB36 genes
title_fullStr Genome-wide characterization and identification of root development and stress-related CsMYB36 genes
title_full_unstemmed Genome-wide characterization and identification of root development and stress-related CsMYB36 genes
title_short Genome-wide characterization and identification of root development and stress-related CsMYB36 genes
title_sort genome wide characterization and identification of root development and stress related csmyb36 genes
topic cucumber
csmyb36
expression profles
gene family
url https://www.maxapress.com/article/doi/10.48130/VR-2023-0019
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