Silicate-substituted bovine-derived hydroxyapatite as a bone substitute in regenerative dentistry
Hydroxyapatite, renowned for its biocompatibility and osteoconductive properties, plays a fundamental role in bone regeneration owing to its resemblance to natural bone mineral, thus offering considerable potential for advancing tissue engineering strategies. In this article, the innovative integrat...
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| Format: | Article |
| Language: | English |
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SAGE Publishing
2025-01-01
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| Series: | Journal of Applied Biomaterials & Functional Materials |
| Online Access: | https://doi.org/10.1177/22808000251314302 |
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| author | Jithendra Ratnayake Niranjan Ramesh Maree L Gould Michael R Mucalo George J Dias |
| author_facet | Jithendra Ratnayake Niranjan Ramesh Maree L Gould Michael R Mucalo George J Dias |
| author_sort | Jithendra Ratnayake |
| collection | DOAJ |
| description | Hydroxyapatite, renowned for its biocompatibility and osteoconductive properties, plays a fundamental role in bone regeneration owing to its resemblance to natural bone mineral, thus offering considerable potential for advancing tissue engineering strategies. In this article, the innovative integration of silicon ions into biogenic (bovine-derived) hydroxyapatite (SiBHA) via a tailored sol-gel process is reported. The resultant SiBHA scaffolds exhibited an interconnected microporous structure with a total porosity of 70% and pore dimensions ranging from 120 to 650 µm. Fourier-transform infrared spectroscopy and X-ray diffraction studies validated the effective incorporation of silicon ions into the BHA lattice, with energy-dispersive X-ray and inductively-coupled plasma mass spectrometry further confirming a Ca/P molar ratio for SiBHA between 1.63 and 1.74. Moreover, SiBHA scaffolds demonstrated commendable chemical and thermal stability. Of note, SiBHA scaffolds were found to display significantly enhanced mechanical properties, including compressive strength and Young’s modulus, compared to the control BHA scaffolds. In vitro assessments highlighted the capacity of SiBHA scaffolds to foster cell viability, proliferation, and osteogenic differentiation of Saos-2 cells. Immunohistochemical analysis revealed a significant increase in osteonectin expression, a key bone matrix protein, after 14 days of incubation under osteogenic conditions. These findings highlight the biocompatibility and therapeutic potential of SiBHA scaffolds, suggesting their suitability as biomaterials for dental bone regeneration applications. |
| format | Article |
| id | doaj-art-bdf501e6ce234a88bd87c395c43249c3 |
| institution | Kabale University |
| issn | 2280-8000 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | SAGE Publishing |
| record_format | Article |
| series | Journal of Applied Biomaterials & Functional Materials |
| spelling | doaj-art-bdf501e6ce234a88bd87c395c43249c32025-01-23T10:03:21ZengSAGE PublishingJournal of Applied Biomaterials & Functional Materials2280-80002025-01-012310.1177/22808000251314302Silicate-substituted bovine-derived hydroxyapatite as a bone substitute in regenerative dentistryJithendra Ratnayake0Niranjan Ramesh1Maree L Gould2Michael R Mucalo3George J Dias4Faculty of Dentistry, Department of Oral Science, University of Otago, Dunedin, New ZealandDepartment of Anatomy, School of Biomedical Sciences, University of Otago, Dunedin, New ZealandFaculty of Dentistry, Department of Oral Science, University of Otago, Dunedin, New ZealandSchool of Science, University of Waikato, Hamilton, New ZealandDepartment of Anatomy, School of Biomedical Sciences, University of Otago, Dunedin, New ZealandHydroxyapatite, renowned for its biocompatibility and osteoconductive properties, plays a fundamental role in bone regeneration owing to its resemblance to natural bone mineral, thus offering considerable potential for advancing tissue engineering strategies. In this article, the innovative integration of silicon ions into biogenic (bovine-derived) hydroxyapatite (SiBHA) via a tailored sol-gel process is reported. The resultant SiBHA scaffolds exhibited an interconnected microporous structure with a total porosity of 70% and pore dimensions ranging from 120 to 650 µm. Fourier-transform infrared spectroscopy and X-ray diffraction studies validated the effective incorporation of silicon ions into the BHA lattice, with energy-dispersive X-ray and inductively-coupled plasma mass spectrometry further confirming a Ca/P molar ratio for SiBHA between 1.63 and 1.74. Moreover, SiBHA scaffolds demonstrated commendable chemical and thermal stability. Of note, SiBHA scaffolds were found to display significantly enhanced mechanical properties, including compressive strength and Young’s modulus, compared to the control BHA scaffolds. In vitro assessments highlighted the capacity of SiBHA scaffolds to foster cell viability, proliferation, and osteogenic differentiation of Saos-2 cells. Immunohistochemical analysis revealed a significant increase in osteonectin expression, a key bone matrix protein, after 14 days of incubation under osteogenic conditions. These findings highlight the biocompatibility and therapeutic potential of SiBHA scaffolds, suggesting their suitability as biomaterials for dental bone regeneration applications.https://doi.org/10.1177/22808000251314302 |
| spellingShingle | Jithendra Ratnayake Niranjan Ramesh Maree L Gould Michael R Mucalo George J Dias Silicate-substituted bovine-derived hydroxyapatite as a bone substitute in regenerative dentistry Journal of Applied Biomaterials & Functional Materials |
| title | Silicate-substituted bovine-derived hydroxyapatite as a bone substitute in regenerative dentistry |
| title_full | Silicate-substituted bovine-derived hydroxyapatite as a bone substitute in regenerative dentistry |
| title_fullStr | Silicate-substituted bovine-derived hydroxyapatite as a bone substitute in regenerative dentistry |
| title_full_unstemmed | Silicate-substituted bovine-derived hydroxyapatite as a bone substitute in regenerative dentistry |
| title_short | Silicate-substituted bovine-derived hydroxyapatite as a bone substitute in regenerative dentistry |
| title_sort | silicate substituted bovine derived hydroxyapatite as a bone substitute in regenerative dentistry |
| url | https://doi.org/10.1177/22808000251314302 |
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