The Use of Specific Non-<i>Saccharomyces</i> Yeasts as Sustainable Biocontrol Solutions Against <i>Botrytis cinerea</i> on Apples and Strawberries
Apples and strawberries hold significant commercial and nutritional value but face pre- and post-harvest spoilage due to infections by <i>Botrytis cinerea.</i> While spoilage is conventionally managed using synthetic chemicals, there is a growing interest in utilising yeasts as biologica...
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2025-01-01
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| author | Zukisani Gomomo Morris Fanadzo Maxwell Mewa-Ngongang Boredi Silas Chidi Justin Wallace Hoff Marieta van der Rijst Lucky Mokwena Mathabatha Evodia Setati Heinrich Wilbur du Plessis |
| author_facet | Zukisani Gomomo Morris Fanadzo Maxwell Mewa-Ngongang Boredi Silas Chidi Justin Wallace Hoff Marieta van der Rijst Lucky Mokwena Mathabatha Evodia Setati Heinrich Wilbur du Plessis |
| author_sort | Zukisani Gomomo |
| collection | DOAJ |
| description | Apples and strawberries hold significant commercial and nutritional value but face pre- and post-harvest spoilage due to infections by <i>Botrytis cinerea.</i> While spoilage is conventionally managed using synthetic chemicals, there is a growing interest in utilising yeasts as biological control agents. This study aimed to assess the antifungal potential of non-<i>Saccharomyces</i> yeasts <i>Suhomyces pyralidae, Meyerozyma guilliermondii, Pichia kluyveri</i>, <i>Zygoascus hellenicus</i>, and <i>Aureobasidium melanogenum</i> against three <i>B. cinerea</i> strains (B05.10, IWBT-FF1, and PPRI 30807) on agar plates and in post-harvest trials on apples and strawberries. <i>Aureobasidium melanogenum</i> exhibited a broad range of extracellular enzyme production and inhibition rates of 55%, 52%, and 40% against the strains. In volatile organic compound (VOC) assays, <i>P. kluyveri</i> and <i>S. pyralidae</i> achieved 79% and 56% inhibition, respectively, with VOCs like isobutanol, isoamyl alcohol, 2-phenylethanol, isoamyl acetate, and 2-phenethyl acetate identified. In post-harvest trials, <i>S. pyralidae</i> was most effective on apples, with inhibition rates up to of 64%. The commercial fungicide Captan and <i>S. pyralidae</i> and <i>P. kluyveri</i> achieved 100% inhibition against the <i>B. cinerea</i> strains B05.10 and IWBT-FF1 on strawberries. These findings highlight the potential of the selected yeast species as biological control agents against <i>B. cinerea</i>, warranting further research into their application in commercial fruit protection. |
| format | Article |
| id | doaj-art-bdc35a76e21a4feea5786fa54b0a0d49 |
| institution | Kabale University |
| issn | 2309-608X |
| language | English |
| publishDate | 2025-01-01 |
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| series | Journal of Fungi |
| spelling | doaj-art-bdc35a76e21a4feea5786fa54b0a0d492025-01-24T13:37:16ZengMDPI AGJournal of Fungi2309-608X2025-01-011112610.3390/jof11010026The Use of Specific Non-<i>Saccharomyces</i> Yeasts as Sustainable Biocontrol Solutions Against <i>Botrytis cinerea</i> on Apples and StrawberriesZukisani Gomomo0Morris Fanadzo1Maxwell Mewa-Ngongang2Boredi Silas Chidi3Justin Wallace Hoff4Marieta van der Rijst5Lucky Mokwena6Mathabatha Evodia Setati7Heinrich Wilbur du Plessis8Post-Harvest and Agro-Processing Technologies Division, ARC Infruitec-Nietvoorbij, Stellenbosch 7599, South AfricaDepartment of Agriculture, Cape Peninsula University of Technology, Wellington 7654, South AfricaPost-Harvest and Agro-Processing Technologies Division, ARC Infruitec-Nietvoorbij, Stellenbosch 7599, South AfricaDepartment of Biotechnology and Consumer Sciences, Cape Peninsula University of Technology, Cape Town 8000, South AfricaPost-Harvest and Agro-Processing Technologies Division, ARC Infruitec-Nietvoorbij, Stellenbosch 7599, South AfricaARC Biometry, ARC Infruitec-Nietvoorbij, Stellenbosch 7599, South AfricaCentral Analytical Facilities, Stellenbosch University, Stellenbosch 7602, South AfricaDepartment of Viticulture and Oenology, South African Grape and Wine Research Institute, Stellenbosch University, Stellenbosch 7602, South AfricaPost-Harvest and Agro-Processing Technologies Division, ARC Infruitec-Nietvoorbij, Stellenbosch 7599, South AfricaApples and strawberries hold significant commercial and nutritional value but face pre- and post-harvest spoilage due to infections by <i>Botrytis cinerea.</i> While spoilage is conventionally managed using synthetic chemicals, there is a growing interest in utilising yeasts as biological control agents. This study aimed to assess the antifungal potential of non-<i>Saccharomyces</i> yeasts <i>Suhomyces pyralidae, Meyerozyma guilliermondii, Pichia kluyveri</i>, <i>Zygoascus hellenicus</i>, and <i>Aureobasidium melanogenum</i> against three <i>B. cinerea</i> strains (B05.10, IWBT-FF1, and PPRI 30807) on agar plates and in post-harvest trials on apples and strawberries. <i>Aureobasidium melanogenum</i> exhibited a broad range of extracellular enzyme production and inhibition rates of 55%, 52%, and 40% against the strains. In volatile organic compound (VOC) assays, <i>P. kluyveri</i> and <i>S. pyralidae</i> achieved 79% and 56% inhibition, respectively, with VOCs like isobutanol, isoamyl alcohol, 2-phenylethanol, isoamyl acetate, and 2-phenethyl acetate identified. In post-harvest trials, <i>S. pyralidae</i> was most effective on apples, with inhibition rates up to of 64%. The commercial fungicide Captan and <i>S. pyralidae</i> and <i>P. kluyveri</i> achieved 100% inhibition against the <i>B. cinerea</i> strains B05.10 and IWBT-FF1 on strawberries. These findings highlight the potential of the selected yeast species as biological control agents against <i>B. cinerea</i>, warranting further research into their application in commercial fruit protection.https://www.mdpi.com/2309-608X/11/1/26mould spoilagesynthetic chemicalspre- and post-harvest controlgrowth inhibitionhydrolytic enzymes |
| spellingShingle | Zukisani Gomomo Morris Fanadzo Maxwell Mewa-Ngongang Boredi Silas Chidi Justin Wallace Hoff Marieta van der Rijst Lucky Mokwena Mathabatha Evodia Setati Heinrich Wilbur du Plessis The Use of Specific Non-<i>Saccharomyces</i> Yeasts as Sustainable Biocontrol Solutions Against <i>Botrytis cinerea</i> on Apples and Strawberries Journal of Fungi mould spoilage synthetic chemicals pre- and post-harvest control growth inhibition hydrolytic enzymes |
| title | The Use of Specific Non-<i>Saccharomyces</i> Yeasts as Sustainable Biocontrol Solutions Against <i>Botrytis cinerea</i> on Apples and Strawberries |
| title_full | The Use of Specific Non-<i>Saccharomyces</i> Yeasts as Sustainable Biocontrol Solutions Against <i>Botrytis cinerea</i> on Apples and Strawberries |
| title_fullStr | The Use of Specific Non-<i>Saccharomyces</i> Yeasts as Sustainable Biocontrol Solutions Against <i>Botrytis cinerea</i> on Apples and Strawberries |
| title_full_unstemmed | The Use of Specific Non-<i>Saccharomyces</i> Yeasts as Sustainable Biocontrol Solutions Against <i>Botrytis cinerea</i> on Apples and Strawberries |
| title_short | The Use of Specific Non-<i>Saccharomyces</i> Yeasts as Sustainable Biocontrol Solutions Against <i>Botrytis cinerea</i> on Apples and Strawberries |
| title_sort | use of specific non i saccharomyces i yeasts as sustainable biocontrol solutions against i botrytis cinerea i on apples and strawberries |
| topic | mould spoilage synthetic chemicals pre- and post-harvest control growth inhibition hydrolytic enzymes |
| url | https://www.mdpi.com/2309-608X/11/1/26 |
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