VvD14c-VvMAX2-VvLOB/VvLBD19 module is involved in the strigolactone-mediated regulation of grapevine root architecture
Abstract The D14 protein, an alpha/beta hydrolase, is a key receptor in the strigolactone (SL) signaling pathway. However, the response of VvD14 to SL signals and its role in grapevine root architecture formation remain unclear. This study demonstrated that VvD14c was highly expressed in grapevine t...
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BMC
2024-10-01
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| Series: | Molecular Horticulture |
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| Online Access: | https://doi.org/10.1186/s43897-024-00117-z |
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| author | Yan Xu Zhengxin Lv Muhammad Aamir Manzoor Linhong Song Maosen Wang Lei Wang Shiping Wang Caixi Zhang Songtao Jiu |
| author_facet | Yan Xu Zhengxin Lv Muhammad Aamir Manzoor Linhong Song Maosen Wang Lei Wang Shiping Wang Caixi Zhang Songtao Jiu |
| author_sort | Yan Xu |
| collection | DOAJ |
| description | Abstract The D14 protein, an alpha/beta hydrolase, is a key receptor in the strigolactone (SL) signaling pathway. However, the response of VvD14 to SL signals and its role in grapevine root architecture formation remain unclear. This study demonstrated that VvD14c was highly expressed in grapevine tissues and fruit stages than other VvD14 isoforms. Application of GR24, an SL analog, enhanced the elongation and diameter of adventitious roots but inhibited the elongation and density of lateral roots (LRs) and increased VvD14c expression. Additionally, GR24 is nested within the VvD14c pocket and strongly bound to the VvD14c protein, with an affinity of 5.65 × 10−9 M. Furthermore, VvD14c interacted with grapevine MORE AXILLARY GROWTH 2 (VvMAX2) in a GR24-dependent manner. Overexpression of VvD14c in the d14 mutant and VvMAX2 in the max2 Arabidopsis mutant reversed the increased LR number and density, as well as primary root elongation. Conversely, homologous overexpression of VvD14c and VvMAX2 resulted in reduced LR number and density in grapevines. VvMAX2 directly interacted with LATERAL ORGAN BOUNDARY (VvLOB) and VvLBD19, thereby positively regulating LR density. These findings highlight the role of SLs in regulating grapevine root architecture, potentially via the VvD14c-VvMAX2-VvLOB/VvLBD19 module, providing new insights into the regulation of root growth and development in grapevines. |
| format | Article |
| id | doaj-art-bdbc8fdc078a4fa48da7108e78625f3d |
| institution | OA Journals |
| issn | 2730-9401 |
| language | English |
| publishDate | 2024-10-01 |
| publisher | BMC |
| record_format | Article |
| series | Molecular Horticulture |
| spelling | doaj-art-bdbc8fdc078a4fa48da7108e78625f3d2025-08-20T02:11:47ZengBMCMolecular Horticulture2730-94012024-10-014111610.1186/s43897-024-00117-zVvD14c-VvMAX2-VvLOB/VvLBD19 module is involved in the strigolactone-mediated regulation of grapevine root architectureYan Xu0Zhengxin Lv1Muhammad Aamir Manzoor2Linhong Song3Maosen Wang4Lei Wang5Shiping Wang6Caixi Zhang7Songtao Jiu8Department of Plant Science, School of Agriculture and Biology, Shanghai Jiao Tong UniversityDepartment of Plant Science, School of Agriculture and Biology, Shanghai Jiao Tong UniversityDepartment of Plant Science, School of Agriculture and Biology, Shanghai Jiao Tong UniversityDepartment of Plant Science, School of Agriculture and Biology, Shanghai Jiao Tong UniversityDepartment of Plant Science, School of Agriculture and Biology, Shanghai Jiao Tong UniversityDepartment of Plant Science, School of Agriculture and Biology, Shanghai Jiao Tong UniversityDepartment of Plant Science, School of Agriculture and Biology, Shanghai Jiao Tong UniversityDepartment of Plant Science, School of Agriculture and Biology, Shanghai Jiao Tong UniversityDepartment of Plant Science, School of Agriculture and Biology, Shanghai Jiao Tong UniversityAbstract The D14 protein, an alpha/beta hydrolase, is a key receptor in the strigolactone (SL) signaling pathway. However, the response of VvD14 to SL signals and its role in grapevine root architecture formation remain unclear. This study demonstrated that VvD14c was highly expressed in grapevine tissues and fruit stages than other VvD14 isoforms. Application of GR24, an SL analog, enhanced the elongation and diameter of adventitious roots but inhibited the elongation and density of lateral roots (LRs) and increased VvD14c expression. Additionally, GR24 is nested within the VvD14c pocket and strongly bound to the VvD14c protein, with an affinity of 5.65 × 10−9 M. Furthermore, VvD14c interacted with grapevine MORE AXILLARY GROWTH 2 (VvMAX2) in a GR24-dependent manner. Overexpression of VvD14c in the d14 mutant and VvMAX2 in the max2 Arabidopsis mutant reversed the increased LR number and density, as well as primary root elongation. Conversely, homologous overexpression of VvD14c and VvMAX2 resulted in reduced LR number and density in grapevines. VvMAX2 directly interacted with LATERAL ORGAN BOUNDARY (VvLOB) and VvLBD19, thereby positively regulating LR density. These findings highlight the role of SLs in regulating grapevine root architecture, potentially via the VvD14c-VvMAX2-VvLOB/VvLBD19 module, providing new insights into the regulation of root growth and development in grapevines.https://doi.org/10.1186/s43897-024-00117-zGrapevinesStrigolactonesRoot architectureDWARF 14MORE AXILLARY GROWTH 2 |
| spellingShingle | Yan Xu Zhengxin Lv Muhammad Aamir Manzoor Linhong Song Maosen Wang Lei Wang Shiping Wang Caixi Zhang Songtao Jiu VvD14c-VvMAX2-VvLOB/VvLBD19 module is involved in the strigolactone-mediated regulation of grapevine root architecture Molecular Horticulture Grapevines Strigolactones Root architecture DWARF 14 MORE AXILLARY GROWTH 2 |
| title | VvD14c-VvMAX2-VvLOB/VvLBD19 module is involved in the strigolactone-mediated regulation of grapevine root architecture |
| title_full | VvD14c-VvMAX2-VvLOB/VvLBD19 module is involved in the strigolactone-mediated regulation of grapevine root architecture |
| title_fullStr | VvD14c-VvMAX2-VvLOB/VvLBD19 module is involved in the strigolactone-mediated regulation of grapevine root architecture |
| title_full_unstemmed | VvD14c-VvMAX2-VvLOB/VvLBD19 module is involved in the strigolactone-mediated regulation of grapevine root architecture |
| title_short | VvD14c-VvMAX2-VvLOB/VvLBD19 module is involved in the strigolactone-mediated regulation of grapevine root architecture |
| title_sort | vvd14c vvmax2 vvlob vvlbd19 module is involved in the strigolactone mediated regulation of grapevine root architecture |
| topic | Grapevines Strigolactones Root architecture DWARF 14 MORE AXILLARY GROWTH 2 |
| url | https://doi.org/10.1186/s43897-024-00117-z |
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