Screening semen samples for Zika virus infection: role for serologic and RT-PCR testing
IntroductionZika virus (ZIKV) is primarily transmitted through mosquito bites though may act as a sexually transmitted infection. Men can shed the virus in their semen for extended periods, with reports indicating shedding for up to six months after the initial infection. This poses a concern for co...
Saved in:
| Main Authors: | , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2025-01-01
|
| Series: | Frontiers in Tropical Diseases |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fitd.2024.1489647/full |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832582914201616384 |
|---|---|
| author | Humberto Ferraz Humberto Ferraz Humberto Ferraz Kevan Akrami Kevan Akrami Kevan Akrami Laise de Moraes Laise de Moraes Lais Cambuí Gusmão Genevieve Coelho Daniele Freitas Daniele Freitas Manoel Barral-Netto Aldina Barral Viviane Boaventura Viviane Boaventura Luciane Amorim Santos Luciane Amorim Santos Luciane Amorim Santos Ricardo Khouri Ricardo Khouri |
| author_facet | Humberto Ferraz Humberto Ferraz Humberto Ferraz Kevan Akrami Kevan Akrami Kevan Akrami Laise de Moraes Laise de Moraes Lais Cambuí Gusmão Genevieve Coelho Daniele Freitas Daniele Freitas Manoel Barral-Netto Aldina Barral Viviane Boaventura Viviane Boaventura Luciane Amorim Santos Luciane Amorim Santos Luciane Amorim Santos Ricardo Khouri Ricardo Khouri |
| author_sort | Humberto Ferraz |
| collection | DOAJ |
| description | IntroductionZika virus (ZIKV) is primarily transmitted through mosquito bites though may act as a sexually transmitted infection. Men can shed the virus in their semen for extended periods, with reports indicating shedding for up to six months after the initial infection. This poses a concern for couples planning pregnancy, whether through natural or assisted methods due to the risk of congenital Zika disease. Human reproductive clinics typically perform serologic tests to screen for Zika infection.MethodsIn this study, we evaluated semen samples stored in a human reproduction clinic in Salvador, Brazil, during the Zika virus outbreak, using real-time quantitative reverse transcription polymerase chain reaction (RT-qPCR) technique.Results36 sperm samples from 23 donors were analyzed, including 5 with positive IgG serology for ZIKV and all negative for IgM. Despite these serologic findings, all analyzed samples were negative by RT-qPCR ZIKV.DiscussionDespite the limited number of samples, this study raises further concern that the use of serology may be an unreliable surrogate method to predict the presence of ZIKV in semen. |
| format | Article |
| id | doaj-art-bdbaf21559d74571986cea704e3ae98c |
| institution | Kabale University |
| issn | 2673-7515 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Tropical Diseases |
| spelling | doaj-art-bdbaf21559d74571986cea704e3ae98c2025-01-29T06:45:50ZengFrontiers Media S.A.Frontiers in Tropical Diseases2673-75152025-01-01510.3389/fitd.2024.14896471489647Screening semen samples for Zika virus infection: role for serologic and RT-PCR testingHumberto Ferraz0Humberto Ferraz1Humberto Ferraz2Kevan Akrami3Kevan Akrami4Kevan Akrami5Laise de Moraes6Laise de Moraes7Lais Cambuí Gusmão8Genevieve Coelho9Daniele Freitas10Daniele Freitas11Manoel Barral-Netto12Aldina Barral13Viviane Boaventura14Viviane Boaventura15Luciane Amorim Santos16Luciane Amorim Santos17Luciane Amorim Santos18Ricardo Khouri19Ricardo Khouri20Departamento de Ciências da Vida, Curso de Medicina, Universidade do Estado da Bahia (UNEB), Salvador, BA, BrazilDepartamento de Patologia e Medicina Legal, Faculdade de Medicina, Universidade Federal da Bahia (UFBA), Salvador, BA, BrazilInstituto Gonçalo Moniz (IGM), Fundação Oswaldo Cruz (FIOCRUZ), Salvador, BA, BrazilDepartamento de Patologia e Medicina Legal, Faculdade de Medicina, Universidade Federal da Bahia (UFBA), Salvador, BA, BrazilInstituto Gonçalo Moniz (IGM), Fundação Oswaldo Cruz (FIOCRUZ), Salvador, BA, BrazilDepartment of Medicine, Faculty of Medicine, University of California, San Diego, San Diego, CA, United StatesDepartamento de Patologia e Medicina Legal, Faculdade de Medicina, Universidade Federal da Bahia (UFBA), Salvador, BA, BrazilInstituto Gonçalo Moniz (IGM), Fundação Oswaldo Cruz (FIOCRUZ), Salvador, BA, BrazilInstituto Gonçalo Moniz (IGM), Fundação Oswaldo Cruz (FIOCRUZ), Salvador, BA, BrazilInstituto Valenciano de Infertilidade (IVI), Salvador, BA, BrazilInstituto Valenciano de Infertilidade (IVI), Salvador, BA, BrazilDepartamento de Biomedicina, Escola Bahiana de Medicina e Saúde Pública (EBMSP), Salvador, BA, BrazilInstituto Gonçalo Moniz (IGM), Fundação Oswaldo Cruz (FIOCRUZ), Salvador, BA, BrazilInstituto Gonçalo Moniz (IGM), Fundação Oswaldo Cruz (FIOCRUZ), Salvador, BA, BrazilDepartamento de Patologia e Medicina Legal, Faculdade de Medicina, Universidade Federal da Bahia (UFBA), Salvador, BA, BrazilInstituto Gonçalo Moniz (IGM), Fundação Oswaldo Cruz (FIOCRUZ), Salvador, BA, BrazilDepartamento de Patologia e Medicina Legal, Faculdade de Medicina, Universidade Federal da Bahia (UFBA), Salvador, BA, BrazilInstituto Gonçalo Moniz (IGM), Fundação Oswaldo Cruz (FIOCRUZ), Salvador, BA, BrazilDepartamento de Biomedicina, Escola Bahiana de Medicina e Saúde Pública (EBMSP), Salvador, BA, BrazilDepartamento de Patologia e Medicina Legal, Faculdade de Medicina, Universidade Federal da Bahia (UFBA), Salvador, BA, BrazilInstituto Gonçalo Moniz (IGM), Fundação Oswaldo Cruz (FIOCRUZ), Salvador, BA, BrazilIntroductionZika virus (ZIKV) is primarily transmitted through mosquito bites though may act as a sexually transmitted infection. Men can shed the virus in their semen for extended periods, with reports indicating shedding for up to six months after the initial infection. This poses a concern for couples planning pregnancy, whether through natural or assisted methods due to the risk of congenital Zika disease. Human reproductive clinics typically perform serologic tests to screen for Zika infection.MethodsIn this study, we evaluated semen samples stored in a human reproduction clinic in Salvador, Brazil, during the Zika virus outbreak, using real-time quantitative reverse transcription polymerase chain reaction (RT-qPCR) technique.Results36 sperm samples from 23 donors were analyzed, including 5 with positive IgG serology for ZIKV and all negative for IgM. Despite these serologic findings, all analyzed samples were negative by RT-qPCR ZIKV.DiscussionDespite the limited number of samples, this study raises further concern that the use of serology may be an unreliable surrogate method to predict the presence of ZIKV in semen.https://www.frontiersin.org/articles/10.3389/fitd.2024.1489647/fullZika virussemengenital tractsexual transmissiondiagnosis |
| spellingShingle | Humberto Ferraz Humberto Ferraz Humberto Ferraz Kevan Akrami Kevan Akrami Kevan Akrami Laise de Moraes Laise de Moraes Lais Cambuí Gusmão Genevieve Coelho Daniele Freitas Daniele Freitas Manoel Barral-Netto Aldina Barral Viviane Boaventura Viviane Boaventura Luciane Amorim Santos Luciane Amorim Santos Luciane Amorim Santos Ricardo Khouri Ricardo Khouri Screening semen samples for Zika virus infection: role for serologic and RT-PCR testing Frontiers in Tropical Diseases Zika virus semen genital tract sexual transmission diagnosis |
| title | Screening semen samples for Zika virus infection: role for serologic and RT-PCR testing |
| title_full | Screening semen samples for Zika virus infection: role for serologic and RT-PCR testing |
| title_fullStr | Screening semen samples for Zika virus infection: role for serologic and RT-PCR testing |
| title_full_unstemmed | Screening semen samples for Zika virus infection: role for serologic and RT-PCR testing |
| title_short | Screening semen samples for Zika virus infection: role for serologic and RT-PCR testing |
| title_sort | screening semen samples for zika virus infection role for serologic and rt pcr testing |
| topic | Zika virus semen genital tract sexual transmission diagnosis |
| url | https://www.frontiersin.org/articles/10.3389/fitd.2024.1489647/full |
| work_keys_str_mv | AT humbertoferraz screeningsemensamplesforzikavirusinfectionroleforserologicandrtpcrtesting AT humbertoferraz screeningsemensamplesforzikavirusinfectionroleforserologicandrtpcrtesting AT humbertoferraz screeningsemensamplesforzikavirusinfectionroleforserologicandrtpcrtesting AT kevanakrami screeningsemensamplesforzikavirusinfectionroleforserologicandrtpcrtesting AT kevanakrami screeningsemensamplesforzikavirusinfectionroleforserologicandrtpcrtesting AT kevanakrami screeningsemensamplesforzikavirusinfectionroleforserologicandrtpcrtesting AT laisedemoraes screeningsemensamplesforzikavirusinfectionroleforserologicandrtpcrtesting AT laisedemoraes screeningsemensamplesforzikavirusinfectionroleforserologicandrtpcrtesting AT laiscambuigusmao screeningsemensamplesforzikavirusinfectionroleforserologicandrtpcrtesting AT genevievecoelho screeningsemensamplesforzikavirusinfectionroleforserologicandrtpcrtesting AT danielefreitas screeningsemensamplesforzikavirusinfectionroleforserologicandrtpcrtesting AT danielefreitas screeningsemensamplesforzikavirusinfectionroleforserologicandrtpcrtesting AT manoelbarralnetto screeningsemensamplesforzikavirusinfectionroleforserologicandrtpcrtesting AT aldinabarral screeningsemensamplesforzikavirusinfectionroleforserologicandrtpcrtesting AT vivianeboaventura screeningsemensamplesforzikavirusinfectionroleforserologicandrtpcrtesting AT vivianeboaventura screeningsemensamplesforzikavirusinfectionroleforserologicandrtpcrtesting AT lucianeamorimsantos screeningsemensamplesforzikavirusinfectionroleforserologicandrtpcrtesting AT lucianeamorimsantos screeningsemensamplesforzikavirusinfectionroleforserologicandrtpcrtesting AT lucianeamorimsantos screeningsemensamplesforzikavirusinfectionroleforserologicandrtpcrtesting AT ricardokhouri screeningsemensamplesforzikavirusinfectionroleforserologicandrtpcrtesting AT ricardokhouri screeningsemensamplesforzikavirusinfectionroleforserologicandrtpcrtesting |