Effect of culture conditions on xylanase activity produced from biocontrol agent Trichoderma harzianum strains
Xylanase produced from Trichoderma spp. can induce plant resistance, which is one of important biocontrol mechanisms of fungal Trichoderma. The effect of cultural condition on the producing xylanase by T. harzianum strains was investigated. Appropriate pH, temperature, concentration of surface activ...
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| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Zhejiang University Press
2009-03-01
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| Series: | 浙江大学学报. 农业与生命科学版 |
| Subjects: | |
| Online Access: | https://www.academax.com/doi/10.3785/j.issn.1008-9209.2009.02.004 |
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| Summary: | Xylanase produced from Trichoderma spp. can induce plant resistance, which is one of important biocontrol mechanisms of fungal Trichoderma. The effect of cultural condition on the producing xylanase by T. harzianum strains was investigated. Appropriate pH, temperature, concentration of surface active agent Tween 80, incubation period were optimized for enzyme production. The results show that the culture conditions of maximum enzyme activities produced from different T. harzianum strains were different in shaker flasks with semi-solid media. Maximum enzyme activities were at pH 5.0, 30 ℃, 0.7%(concentration of surface active agent Tween 80) and 72 h for NF<sub>9</sub>, pH 4.0, 27 ℃, 1.2% and 84 h for P<sub>1</sub>, pH 5.0, 27 ℃, 0.7% and 84 h for CT<sub>22</sub>, pH 4.0, 30 ℃, 0.7% and 96 h for TC<sub>3</sub>, respectively. |
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| ISSN: | 1008-9209 2097-5155 |