The Effects of Immunosuppressive Factors on Primary Dendritic Cells from C57BL/6 and CBA Mice
Introduction. Dendritic cells (DCs) control immune responses by modulating T and B cells towards effector or tolerogenic responses. In this study, we evaluated the effects of different immunosuppressive molecules on the phenotypic and functional characteristics of primary dendritic cells from C57BL/...
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| Format: | Article |
| Language: | English |
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Wiley
2019-01-01
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| Series: | Journal of Immunology Research |
| Online Access: | http://dx.doi.org/10.1155/2019/7029726 |
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| author | Vasiliy V. Kurilin Julia N. Khantakova Valeriy P. Tereschenko Julia A. Lopatnikova Irina A. Obleukhova Sergey V. Sennikov |
| author_facet | Vasiliy V. Kurilin Julia N. Khantakova Valeriy P. Tereschenko Julia A. Lopatnikova Irina A. Obleukhova Sergey V. Sennikov |
| author_sort | Vasiliy V. Kurilin |
| collection | DOAJ |
| description | Introduction. Dendritic cells (DCs) control immune responses by modulating T and B cells towards effector or tolerogenic responses. In this study, we evaluated the effects of different immunosuppressive molecules on the phenotypic and functional characteristics of primary dendritic cells from C57BL/6 and CBA mice. Methods. DCs were derived from bone marrow cells in the presence of rmGM-CSF and rmIL-4. DCs were then treated with different types of immunosuppressive molecules (rmIL-10, rmTGF-β, and BAY 11-7082) and cocultured with syngeneic splenocytes. The amount of CD4+CD25hiFoxP3+ Tregs, IL-10 expression, and proliferation were evaluated. Results. Tolerogenic factors were found to have different effects on DCs C57Bl/6 mice. In C57Bl/6 mice, BAY 11-7082 alone had no effect on the expression of DC maturation molecules (CD80, CD86). Transforming growth factor beta (TGF-β), alone and in combination with BAY 11-7082, reduced the expression of these molecules. Cocultivation of DCs with splenocytes in the presence of TGF-β and BAY 11-7082 favored regulatory T cell (CD4+CD25hiFoxP3+) differentiation and disfavored differentiation of CD4+ T cells producing IL-10. In CBA mice, we found that rmIL-10 and rmTGF-β have a weak effect on maturation of DCs and their functional properties to induce Treg cells and IL-10 production. Conclusion. These results indicate that TGF-β and IL-10 have different effects on the phenotypic and functional characteristics of DCs and that the NF-κB inhibitor, BAY 11-7082, has no synergistic effect on these treatments. In mice with an opposite nature of the immune response, the effects of immunoregulatory cytokines (IL-10 and TGF-b) differ on maturation of dendritic cells. |
| format | Article |
| id | doaj-art-bda261731351479db5deaceda43e6986 |
| institution | Kabale University |
| issn | 2314-8861 2314-7156 |
| language | English |
| publishDate | 2019-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Immunology Research |
| spelling | doaj-art-bda261731351479db5deaceda43e69862025-02-03T06:11:17ZengWileyJournal of Immunology Research2314-88612314-71562019-01-01201910.1155/2019/70297267029726The Effects of Immunosuppressive Factors on Primary Dendritic Cells from C57BL/6 and CBA MiceVasiliy V. Kurilin0Julia N. Khantakova1Valeriy P. Tereschenko2Julia A. Lopatnikova3Irina A. Obleukhova4Sergey V. Sennikov5Laboratory of Molecular Immunology, Federal State Budgetary Institution “Research Institute of Fundamental and Clinical Immunology”, Yadrintsevskaya St. 14, Novosibirsk, 630099, RussiaLaboratory of Molecular Immunology, Federal State Budgetary Institution “Research Institute of Fundamental and Clinical Immunology”, Yadrintsevskaya St. 14, Novosibirsk, 630099, RussiaLaboratory of Molecular Immunology, Federal State Budgetary Institution “Research Institute of Fundamental and Clinical Immunology”, Yadrintsevskaya St. 14, Novosibirsk, 630099, RussiaLaboratory of Molecular Immunology, Federal State Budgetary Institution “Research Institute of Fundamental and Clinical Immunology”, Yadrintsevskaya St. 14, Novosibirsk, 630099, RussiaLaboratory of Molecular Immunology, Federal State Budgetary Institution “Research Institute of Fundamental and Clinical Immunology”, Yadrintsevskaya St. 14, Novosibirsk, 630099, RussiaLaboratory of Molecular Immunology, Federal State Budgetary Institution “Research Institute of Fundamental and Clinical Immunology”, Yadrintsevskaya St. 14, Novosibirsk, 630099, RussiaIntroduction. Dendritic cells (DCs) control immune responses by modulating T and B cells towards effector or tolerogenic responses. In this study, we evaluated the effects of different immunosuppressive molecules on the phenotypic and functional characteristics of primary dendritic cells from C57BL/6 and CBA mice. Methods. DCs were derived from bone marrow cells in the presence of rmGM-CSF and rmIL-4. DCs were then treated with different types of immunosuppressive molecules (rmIL-10, rmTGF-β, and BAY 11-7082) and cocultured with syngeneic splenocytes. The amount of CD4+CD25hiFoxP3+ Tregs, IL-10 expression, and proliferation were evaluated. Results. Tolerogenic factors were found to have different effects on DCs C57Bl/6 mice. In C57Bl/6 mice, BAY 11-7082 alone had no effect on the expression of DC maturation molecules (CD80, CD86). Transforming growth factor beta (TGF-β), alone and in combination with BAY 11-7082, reduced the expression of these molecules. Cocultivation of DCs with splenocytes in the presence of TGF-β and BAY 11-7082 favored regulatory T cell (CD4+CD25hiFoxP3+) differentiation and disfavored differentiation of CD4+ T cells producing IL-10. In CBA mice, we found that rmIL-10 and rmTGF-β have a weak effect on maturation of DCs and their functional properties to induce Treg cells and IL-10 production. Conclusion. These results indicate that TGF-β and IL-10 have different effects on the phenotypic and functional characteristics of DCs and that the NF-κB inhibitor, BAY 11-7082, has no synergistic effect on these treatments. In mice with an opposite nature of the immune response, the effects of immunoregulatory cytokines (IL-10 and TGF-b) differ on maturation of dendritic cells.http://dx.doi.org/10.1155/2019/7029726 |
| spellingShingle | Vasiliy V. Kurilin Julia N. Khantakova Valeriy P. Tereschenko Julia A. Lopatnikova Irina A. Obleukhova Sergey V. Sennikov The Effects of Immunosuppressive Factors on Primary Dendritic Cells from C57BL/6 and CBA Mice Journal of Immunology Research |
| title | The Effects of Immunosuppressive Factors on Primary Dendritic Cells from C57BL/6 and CBA Mice |
| title_full | The Effects of Immunosuppressive Factors on Primary Dendritic Cells from C57BL/6 and CBA Mice |
| title_fullStr | The Effects of Immunosuppressive Factors on Primary Dendritic Cells from C57BL/6 and CBA Mice |
| title_full_unstemmed | The Effects of Immunosuppressive Factors on Primary Dendritic Cells from C57BL/6 and CBA Mice |
| title_short | The Effects of Immunosuppressive Factors on Primary Dendritic Cells from C57BL/6 and CBA Mice |
| title_sort | effects of immunosuppressive factors on primary dendritic cells from c57bl 6 and cba mice |
| url | http://dx.doi.org/10.1155/2019/7029726 |
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