Association of SIGLEC9 Expression with Cytokine Expression, Tumor Grading, KRAS, NRAS, BRAF, PIK3CA, AKT Gene Mutations, and MSI Status in Colorectal Cancer
SIGLEC9 (sialic acid-binding Ig-like lectin 9) is a molecule thought to have a significant influence on the immune properties of the colorectal cancer (CRC) tumor microenvironment (TME). In our study, we assessed the expression of the SIGLEC9 protein in CRC tissue and the surgical margin tissue. Usi...
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2024-11-01
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| author | Błażej Ochman Anna Kot Sylwia Mielcarska Agnieszka Kula Miriam Dawidowicz Dominika Koszewska Dorota Hudy Monika Szrot Jerzy Piecuch Dariusz Waniczek Zenon Czuba Elżbieta Świętochowska |
| author_facet | Błażej Ochman Anna Kot Sylwia Mielcarska Agnieszka Kula Miriam Dawidowicz Dominika Koszewska Dorota Hudy Monika Szrot Jerzy Piecuch Dariusz Waniczek Zenon Czuba Elżbieta Świętochowska |
| author_sort | Błażej Ochman |
| collection | DOAJ |
| description | SIGLEC9 (sialic acid-binding Ig-like lectin 9) is a molecule thought to have a significant influence on the immune properties of the colorectal cancer (CRC) tumor microenvironment (TME). In our study, we assessed the expression of the SIGLEC9 protein in CRC tissue and the surgical margin tissue. Using RT-PCR, we analyzed mutations in the KRAS, NRAS, BRAF, PIK3CA, and AKT genes. We observed a significantly elevated expression of the SIGLEC9 protein in CRC tissue compared to the control group. No significant differences were observed in SIGLEC9 protein expression depending on mutations in the KRAS, NRAS, BRAF, PIK3CA, and AKT genes or microsatellite instability (MSI) status. However, we found a significantly higher expression of the SIGLEC9 protein in high-grade tumors compared to the low-grade tumors group. SIGLEC9 expression was significantly associated with the expression of multiple cytokines, chemokines, and growth factors in the CRC TME. These associations suggest the significant potential of SIGLEC9 as a molecule that plays a crucial role in shaping the immune properties of the CRC TME, as well as its potential therapeutic relevance, particularly in the group of high-grade CRC tumors. |
| format | Article |
| id | doaj-art-bd982bfb208f41cdb36eaa038bfdb5aa |
| institution | DOAJ |
| issn | 1467-3037 1467-3045 |
| language | English |
| publishDate | 2024-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Current Issues in Molecular Biology |
| spelling | doaj-art-bd982bfb208f41cdb36eaa038bfdb5aa2025-08-20T02:55:52ZengMDPI AGCurrent Issues in Molecular Biology1467-30371467-30452024-11-014612136171364610.3390/cimb46120814Association of SIGLEC9 Expression with Cytokine Expression, Tumor Grading, KRAS, NRAS, BRAF, PIK3CA, AKT Gene Mutations, and MSI Status in Colorectal CancerBłażej Ochman0Anna Kot1Sylwia Mielcarska2Agnieszka Kula3Miriam Dawidowicz4Dominika Koszewska5Dorota Hudy6Monika Szrot7Jerzy Piecuch8Dariusz Waniczek9Zenon Czuba10Elżbieta Świętochowska11Department of Medical and Molecular Biology, Faculty of Medical Sciences in Zabrze, Medical University of Silesia, 19 Jordana, 41-808 Zabrze, PolandDepartment of Medical and Molecular Biology, Faculty of Medical Sciences in Zabrze, Medical University of Silesia, 19 Jordana, 41-808 Zabrze, PolandDepartment of Medical and Molecular Biology, Faculty of Medical Sciences in Zabrze, Medical University of Silesia, 19 Jordana, 41-808 Zabrze, PolandDepartment of Oncological Surgery, Faculty of Medical Sciences in Zabrze, Medical University of Silesia, 41-808 Katowice, PolandDepartment of Oncological Surgery, Faculty of Medical Sciences in Zabrze, Medical University of Silesia, 41-808 Katowice, PolandDepartment of Medical and Molecular Biology, Faculty of Medical Sciences in Zabrze, Medical University of Silesia, 19 Jordana, 41-808 Zabrze, PolandDepartment of Medical and Molecular Biology, Faculty of Medical Sciences in Zabrze, Medical University of Silesia, 19 Jordana, 41-808 Zabrze, PolandDepartment of General and Bariatric Surgery and Emergency Medicine in Zabrze, Faculty of Medical Sciences in Zabrze, Medical University of Silesia, 10 Marii Curie-Skłodowskiej, 41-800 Zabrze, PolandDepartment of General and Bariatric Surgery and Emergency Medicine in Zabrze, Faculty of Medical Sciences in Zabrze, Medical University of Silesia, 10 Marii Curie-Skłodowskiej, 41-800 Zabrze, PolandDepartment of Oncological Surgery, Faculty of Medical Sciences in Zabrze, Medical University of Silesia, 41-808 Katowice, PolandDepartment of Microbiology and Immunology, Faculty of Medical Sciences in Zabrze, Medical University of Silesia, 19 Jordana, 41-808 Zabrze, PolandDepartment of Medical and Molecular Biology, Faculty of Medical Sciences in Zabrze, Medical University of Silesia, 19 Jordana, 41-808 Zabrze, PolandSIGLEC9 (sialic acid-binding Ig-like lectin 9) is a molecule thought to have a significant influence on the immune properties of the colorectal cancer (CRC) tumor microenvironment (TME). In our study, we assessed the expression of the SIGLEC9 protein in CRC tissue and the surgical margin tissue. Using RT-PCR, we analyzed mutations in the KRAS, NRAS, BRAF, PIK3CA, and AKT genes. We observed a significantly elevated expression of the SIGLEC9 protein in CRC tissue compared to the control group. No significant differences were observed in SIGLEC9 protein expression depending on mutations in the KRAS, NRAS, BRAF, PIK3CA, and AKT genes or microsatellite instability (MSI) status. However, we found a significantly higher expression of the SIGLEC9 protein in high-grade tumors compared to the low-grade tumors group. SIGLEC9 expression was significantly associated with the expression of multiple cytokines, chemokines, and growth factors in the CRC TME. These associations suggest the significant potential of SIGLEC9 as a molecule that plays a crucial role in shaping the immune properties of the CRC TME, as well as its potential therapeutic relevance, particularly in the group of high-grade CRC tumors.https://www.mdpi.com/1467-3045/46/12/814colorectal cancer (CRC)human sialic-acid-binding immunoglobulin-like lectin-9 (SIGLEC9)microenvironmenttumorK-ras oncogeneproto-oncogene proteins B-raf (BRAF) |
| spellingShingle | Błażej Ochman Anna Kot Sylwia Mielcarska Agnieszka Kula Miriam Dawidowicz Dominika Koszewska Dorota Hudy Monika Szrot Jerzy Piecuch Dariusz Waniczek Zenon Czuba Elżbieta Świętochowska Association of SIGLEC9 Expression with Cytokine Expression, Tumor Grading, KRAS, NRAS, BRAF, PIK3CA, AKT Gene Mutations, and MSI Status in Colorectal Cancer Current Issues in Molecular Biology colorectal cancer (CRC) human sialic-acid-binding immunoglobulin-like lectin-9 (SIGLEC9) microenvironment tumor K-ras oncogene proto-oncogene proteins B-raf (BRAF) |
| title | Association of SIGLEC9 Expression with Cytokine Expression, Tumor Grading, KRAS, NRAS, BRAF, PIK3CA, AKT Gene Mutations, and MSI Status in Colorectal Cancer |
| title_full | Association of SIGLEC9 Expression with Cytokine Expression, Tumor Grading, KRAS, NRAS, BRAF, PIK3CA, AKT Gene Mutations, and MSI Status in Colorectal Cancer |
| title_fullStr | Association of SIGLEC9 Expression with Cytokine Expression, Tumor Grading, KRAS, NRAS, BRAF, PIK3CA, AKT Gene Mutations, and MSI Status in Colorectal Cancer |
| title_full_unstemmed | Association of SIGLEC9 Expression with Cytokine Expression, Tumor Grading, KRAS, NRAS, BRAF, PIK3CA, AKT Gene Mutations, and MSI Status in Colorectal Cancer |
| title_short | Association of SIGLEC9 Expression with Cytokine Expression, Tumor Grading, KRAS, NRAS, BRAF, PIK3CA, AKT Gene Mutations, and MSI Status in Colorectal Cancer |
| title_sort | association of siglec9 expression with cytokine expression tumor grading kras nras braf pik3ca akt gene mutations and msi status in colorectal cancer |
| topic | colorectal cancer (CRC) human sialic-acid-binding immunoglobulin-like lectin-9 (SIGLEC9) microenvironment tumor K-ras oncogene proto-oncogene proteins B-raf (BRAF) |
| url | https://www.mdpi.com/1467-3045/46/12/814 |
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