Electrochemical Determination of Metronidazole in Tablet Samples Using Carbon Paste Electrode

Cyclic voltammetric investigation of metronidazole at carbon paste electrode revealed an irreversible reduction peak centered at about −0.4 V. Observed peak potential shift with pH in the range 2.0 to 8.5 indicated the involvement of protons during the reduction of metronidazole, whereas the peak po...

Full description

Saved in:
Bibliographic Details
Main Authors: Yosef Nikodimos, Meareg Amare
Format: Article
Language:English
Published: Wiley 2016-01-01
Series:Journal of Analytical Methods in Chemistry
Online Access:http://dx.doi.org/10.1155/2016/3612943
Tags: Add Tag
No Tags, Be the first to tag this record!
_version_ 1832554312129052672
author Yosef Nikodimos
Meareg Amare
author_facet Yosef Nikodimos
Meareg Amare
author_sort Yosef Nikodimos
collection DOAJ
description Cyclic voltammetric investigation of metronidazole at carbon paste electrode revealed an irreversible reduction peak centered at about −0.4 V. Observed peak potential shift with pH in the range 2.0 to 8.5 indicated the involvement of protons during the reduction of metronidazole, whereas the peak potential shift with scan rate in the range 10–250 mV/s confirmed the irreversibility of the reduction reaction. A better correlation coefficient for the dependence of peak current on the scan rate than on the square root of scan rate indicated an adsorption controlled kinetics. Under the optimized method and solution parameters, an excellent linearity between the reductive peak current and the concentration of metronidazole was observed in the concentration range 1.0 × 10−6 to 5.0 × 10−4 M with a correlation coefficient, method detection limit (based on s=3σ), and limit of quantification of 0.999, 2.97 × 10−7 M and 9.91 × 10−7 M, respectively. Good recovery results for spiked metronidazole in tablet samples and selective determination of metronidazole in tablet formulations in the presence of selected potential interferents such as rabeprazole, omeprazole, and tinidazole confirmed the potential applicability of the developed method for the determination of metronidazole in real samples like pharmaceutical tablets.
format Article
id doaj-art-bd97e78b777f407baf1b8352ff09a28e
institution Kabale University
issn 2090-8865
2090-8873
language English
publishDate 2016-01-01
publisher Wiley
record_format Article
series Journal of Analytical Methods in Chemistry
spelling doaj-art-bd97e78b777f407baf1b8352ff09a28e2025-02-03T05:51:56ZengWileyJournal of Analytical Methods in Chemistry2090-88652090-88732016-01-01201610.1155/2016/36129433612943Electrochemical Determination of Metronidazole in Tablet Samples Using Carbon Paste ElectrodeYosef Nikodimos0Meareg Amare1Department of Chemistry, Bahir Dar University, P.O. Box 79, Bahr Dar, EthiopiaDepartment of Chemistry, Bahir Dar University, P.O. Box 79, Bahr Dar, EthiopiaCyclic voltammetric investigation of metronidazole at carbon paste electrode revealed an irreversible reduction peak centered at about −0.4 V. Observed peak potential shift with pH in the range 2.0 to 8.5 indicated the involvement of protons during the reduction of metronidazole, whereas the peak potential shift with scan rate in the range 10–250 mV/s confirmed the irreversibility of the reduction reaction. A better correlation coefficient for the dependence of peak current on the scan rate than on the square root of scan rate indicated an adsorption controlled kinetics. Under the optimized method and solution parameters, an excellent linearity between the reductive peak current and the concentration of metronidazole was observed in the concentration range 1.0 × 10−6 to 5.0 × 10−4 M with a correlation coefficient, method detection limit (based on s=3σ), and limit of quantification of 0.999, 2.97 × 10−7 M and 9.91 × 10−7 M, respectively. Good recovery results for spiked metronidazole in tablet samples and selective determination of metronidazole in tablet formulations in the presence of selected potential interferents such as rabeprazole, omeprazole, and tinidazole confirmed the potential applicability of the developed method for the determination of metronidazole in real samples like pharmaceutical tablets.http://dx.doi.org/10.1155/2016/3612943
spellingShingle Yosef Nikodimos
Meareg Amare
Electrochemical Determination of Metronidazole in Tablet Samples Using Carbon Paste Electrode
Journal of Analytical Methods in Chemistry
title Electrochemical Determination of Metronidazole in Tablet Samples Using Carbon Paste Electrode
title_full Electrochemical Determination of Metronidazole in Tablet Samples Using Carbon Paste Electrode
title_fullStr Electrochemical Determination of Metronidazole in Tablet Samples Using Carbon Paste Electrode
title_full_unstemmed Electrochemical Determination of Metronidazole in Tablet Samples Using Carbon Paste Electrode
title_short Electrochemical Determination of Metronidazole in Tablet Samples Using Carbon Paste Electrode
title_sort electrochemical determination of metronidazole in tablet samples using carbon paste electrode
url http://dx.doi.org/10.1155/2016/3612943
work_keys_str_mv AT yosefnikodimos electrochemicaldeterminationofmetronidazoleintabletsamplesusingcarbonpasteelectrode
AT mearegamare electrochemicaldeterminationofmetronidazoleintabletsamplesusingcarbonpasteelectrode