IDENTIFICATION OF Encarsia FÖRSTER (HYMENOPTERA: APHELINIDAE) SPECIES FROM NORTHEASTERN MÉXICO BY CYTOCHROME C OXIDASE SUBUNIT 1 (CO1) SEQUENCING
Background. Aphelinidae, comprising more than 1120 species in 40 genera, are economically significant parasitoids known for preying on Aleyrodidae, Coccidae, Pseudococcidae, and Aphididae. However, there is scarcity of registered sequences for Aphelinidae and the Encarsia genus in Mexico's data...
Saved in:
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Universidad Autónoma de Yucatán
2025-02-01
|
| Series: | Tropical and Subtropical Agroecosystems |
| Subjects: | |
| Online Access: | https://www.revista.ccba.uady.mx/ojs/index.php/TSA/article/view/5083 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1850185283962667008 |
|---|---|
| author | Daniel Alfonso García-Guerrero Oswaldo García-Martínez Svetlana Nikolaevna-Myartseva Lissethe Palomo-Ligas Luis Alberto Aguirre-Uribe Mariano Flores-Dávila Adriana Carolina Flores-Gallegos Raúl Rodríguez-Herrera Hermelindo Hernandez Torres |
| author_facet | Daniel Alfonso García-Guerrero Oswaldo García-Martínez Svetlana Nikolaevna-Myartseva Lissethe Palomo-Ligas Luis Alberto Aguirre-Uribe Mariano Flores-Dávila Adriana Carolina Flores-Gallegos Raúl Rodríguez-Herrera Hermelindo Hernandez Torres |
| author_sort | Daniel Alfonso García-Guerrero |
| collection | DOAJ |
| description | Background. Aphelinidae, comprising more than 1120 species in 40 genera, are economically significant parasitoids known for preying on Aleyrodidae, Coccidae, Pseudococcidae, and Aphididae. However, there is scarcity of registered sequences for Aphelinidae and the Encarsia genus in Mexico's databases. With a widespread distribution, Encarsia stands as the largest genus within this family, boasting 96 species. The application of sequencing techniques, particularly targeting the cytochrome c oxidase subunit 1 (CO1) mitochondrial gene, has considerably eased species identification, providing valuable information at the species level. Objective. To identify Encarsia species through CO1 gene sequencing. By doing so, the study sought to augment the information available for this group in the GenBank and globally, facilitating further taxonomic comprehension and unveiling potential cryptic species. Methodology. During 2015 and 2016, we collected Aleyrodidae and Diaspididae nymphs from various locations, namely Tampico Alto and Ciudad Cuauhtémoc in Veracruz, and Altamira, Tamaulipas, and Saltillo, Coahuila. Taxonomic identification of the species Encarsia citrina, Encarsia perplexa, and Encarsia tamaulipeca was conducted alongside CO1 gene sequencing. The obtained sequences were subsequently deposited in the GenBank under the accession keys MF444685, MF444686, and MF444687, respectively. Results. Through the application of CO1 gene sequencing, we successfully identified three Encarsia species in the regions under investigation. Notably, the registration of the latter two species marked their first-ever presence in the GenBank, further augmenting the knowledge base for this genus on a global scale. Implications. The inclusion of these new sequences in the GenBank represents a significant step forward for Aphelinidae, specifically Encarsia, in Mexico. This expansion of data will serve as a valuable tool for validating traditional identification methods, exploring intra- and interspecies variations, and shedding light on previously unknown cryptic species. Conclusions. By utilizing CO1sequencing, this study successfully identified and registered three Encarsia species in the GenBank, including two species previously unrecorded worldwide. The newfound genetic data will be instrumental in enhancing our understanding of this ecologically important group, contributing to more precise taxonomic assessments and encouraging further investigations into the diversity and distribution of Aphelinidae in Mexico and beyond. |
| format | Article |
| id | doaj-art-bd720ee09f3e4e5eb7e3a98ed23a8bf5 |
| institution | OA Journals |
| issn | 1870-0462 |
| language | English |
| publishDate | 2025-02-01 |
| publisher | Universidad Autónoma de Yucatán |
| record_format | Article |
| series | Tropical and Subtropical Agroecosystems |
| spelling | doaj-art-bd720ee09f3e4e5eb7e3a98ed23a8bf52025-08-20T02:16:46ZengUniversidad Autónoma de YucatánTropical and Subtropical Agroecosystems1870-04622025-02-0128110.56369/tsaes.50831756IDENTIFICATION OF Encarsia FÖRSTER (HYMENOPTERA: APHELINIDAE) SPECIES FROM NORTHEASTERN MÉXICO BY CYTOCHROME C OXIDASE SUBUNIT 1 (CO1) SEQUENCINGDaniel Alfonso García-Guerrero0Oswaldo García-Martínez1Svetlana Nikolaevna-Myartseva2Lissethe Palomo-Ligas3Luis Alberto Aguirre-Uribe4Mariano Flores-Dávila5Adriana Carolina Flores-Gallegos6Raúl Rodríguez-Herrera7Hermelindo Hernandez Torres8Centro de Investigación para los Recursos Naturales (CIReNa), antigua Escuela Normal Rural s/n, CP 33941, Salaices, Municipio de López, ChihuahuaDepartamento de Parasitología Agrícola, Universidad Autónoma Agraria Antonio Narro, Calz Antonio Narro 1923, Buenavista, 25315 Saltillo, CoahuilaFacultad de Ingeniería y Ciencias, Universidad Autónoma de Tamaulipas, Centro Universitario, Campus, 87149. Ciudad Victoria 87149, TamaulipasFacultad de Ciencias Químicas, Universidad Autónoma de Coahuila, Blvd. V. Carranza s/n. Col. República Oriente C.P.25280 Saltillo, CoahuilaDepartamento de Parasitología Agrícola, Universidad Autónoma Agraria Antonio Narro, Calz Antonio Narro 1923, Buenavista, 25315 Saltillo, CoahuilaDepartamento de Parasitología Agrícola, Universidad Autónoma Agraria Antonio Narro, Calz Antonio Narro 1923, Buenavista, 25315 Saltillo, CoahuilaFacultad de Ciencias Químicas, Universidad Autónoma de Coahuila, Blvd. V. Carranza s/n. Col. República Oriente C.P.25280 Saltillo, CoahuilaFacultad de Ciencias Químicas, Universidad Autónoma de Coahuila, Blvd. V. Carranza s/n. Col. República Oriente C.P.25280 Saltillo, CoahuilaUnidad Académica Multidisciplinaria Mante, Universidad Autónoma de Tamaulipas, Boulevard Enrique Cárdenas González 1201 Pte., Col. Jardín, C. P. 89840, Ciudad Mante, TamaulipasBackground. Aphelinidae, comprising more than 1120 species in 40 genera, are economically significant parasitoids known for preying on Aleyrodidae, Coccidae, Pseudococcidae, and Aphididae. However, there is scarcity of registered sequences for Aphelinidae and the Encarsia genus in Mexico's databases. With a widespread distribution, Encarsia stands as the largest genus within this family, boasting 96 species. The application of sequencing techniques, particularly targeting the cytochrome c oxidase subunit 1 (CO1) mitochondrial gene, has considerably eased species identification, providing valuable information at the species level. Objective. To identify Encarsia species through CO1 gene sequencing. By doing so, the study sought to augment the information available for this group in the GenBank and globally, facilitating further taxonomic comprehension and unveiling potential cryptic species. Methodology. During 2015 and 2016, we collected Aleyrodidae and Diaspididae nymphs from various locations, namely Tampico Alto and Ciudad Cuauhtémoc in Veracruz, and Altamira, Tamaulipas, and Saltillo, Coahuila. Taxonomic identification of the species Encarsia citrina, Encarsia perplexa, and Encarsia tamaulipeca was conducted alongside CO1 gene sequencing. The obtained sequences were subsequently deposited in the GenBank under the accession keys MF444685, MF444686, and MF444687, respectively. Results. Through the application of CO1 gene sequencing, we successfully identified three Encarsia species in the regions under investigation. Notably, the registration of the latter two species marked their first-ever presence in the GenBank, further augmenting the knowledge base for this genus on a global scale. Implications. The inclusion of these new sequences in the GenBank represents a significant step forward for Aphelinidae, specifically Encarsia, in Mexico. This expansion of data will serve as a valuable tool for validating traditional identification methods, exploring intra- and interspecies variations, and shedding light on previously unknown cryptic species. Conclusions. By utilizing CO1sequencing, this study successfully identified and registered three Encarsia species in the GenBank, including two species previously unrecorded worldwide. The newfound genetic data will be instrumental in enhancing our understanding of this ecologically important group, contributing to more precise taxonomic assessments and encouraging further investigations into the diversity and distribution of Aphelinidae in Mexico and beyond.https://www.revista.ccba.uady.mx/ojs/index.php/TSA/article/view/5083aphelinidaeencarsiacoi gene sequencingspecies identification |
| spellingShingle | Daniel Alfonso García-Guerrero Oswaldo García-Martínez Svetlana Nikolaevna-Myartseva Lissethe Palomo-Ligas Luis Alberto Aguirre-Uribe Mariano Flores-Dávila Adriana Carolina Flores-Gallegos Raúl Rodríguez-Herrera Hermelindo Hernandez Torres IDENTIFICATION OF Encarsia FÖRSTER (HYMENOPTERA: APHELINIDAE) SPECIES FROM NORTHEASTERN MÉXICO BY CYTOCHROME C OXIDASE SUBUNIT 1 (CO1) SEQUENCING Tropical and Subtropical Agroecosystems aphelinidae encarsia coi gene sequencing species identification |
| title | IDENTIFICATION OF Encarsia FÖRSTER (HYMENOPTERA: APHELINIDAE) SPECIES FROM NORTHEASTERN MÉXICO BY CYTOCHROME C OXIDASE SUBUNIT 1 (CO1) SEQUENCING |
| title_full | IDENTIFICATION OF Encarsia FÖRSTER (HYMENOPTERA: APHELINIDAE) SPECIES FROM NORTHEASTERN MÉXICO BY CYTOCHROME C OXIDASE SUBUNIT 1 (CO1) SEQUENCING |
| title_fullStr | IDENTIFICATION OF Encarsia FÖRSTER (HYMENOPTERA: APHELINIDAE) SPECIES FROM NORTHEASTERN MÉXICO BY CYTOCHROME C OXIDASE SUBUNIT 1 (CO1) SEQUENCING |
| title_full_unstemmed | IDENTIFICATION OF Encarsia FÖRSTER (HYMENOPTERA: APHELINIDAE) SPECIES FROM NORTHEASTERN MÉXICO BY CYTOCHROME C OXIDASE SUBUNIT 1 (CO1) SEQUENCING |
| title_short | IDENTIFICATION OF Encarsia FÖRSTER (HYMENOPTERA: APHELINIDAE) SPECIES FROM NORTHEASTERN MÉXICO BY CYTOCHROME C OXIDASE SUBUNIT 1 (CO1) SEQUENCING |
| title_sort | identification of encarsia forster hymenoptera aphelinidae species from northeastern mexico by cytochrome c oxidase subunit 1 co1 sequencing |
| topic | aphelinidae encarsia coi gene sequencing species identification |
| url | https://www.revista.ccba.uady.mx/ojs/index.php/TSA/article/view/5083 |
| work_keys_str_mv | AT danielalfonsogarciaguerrero identificationofencarsiaforsterhymenopteraaphelinidaespeciesfromnortheasternmexicobycytochromecoxidasesubunit1co1sequencing AT oswaldogarciamartinez identificationofencarsiaforsterhymenopteraaphelinidaespeciesfromnortheasternmexicobycytochromecoxidasesubunit1co1sequencing AT svetlananikolaevnamyartseva identificationofencarsiaforsterhymenopteraaphelinidaespeciesfromnortheasternmexicobycytochromecoxidasesubunit1co1sequencing AT lissethepalomoligas identificationofencarsiaforsterhymenopteraaphelinidaespeciesfromnortheasternmexicobycytochromecoxidasesubunit1co1sequencing AT luisalbertoaguirreuribe identificationofencarsiaforsterhymenopteraaphelinidaespeciesfromnortheasternmexicobycytochromecoxidasesubunit1co1sequencing AT marianofloresdavila identificationofencarsiaforsterhymenopteraaphelinidaespeciesfromnortheasternmexicobycytochromecoxidasesubunit1co1sequencing AT adrianacarolinafloresgallegos identificationofencarsiaforsterhymenopteraaphelinidaespeciesfromnortheasternmexicobycytochromecoxidasesubunit1co1sequencing AT raulrodriguezherrera identificationofencarsiaforsterhymenopteraaphelinidaespeciesfromnortheasternmexicobycytochromecoxidasesubunit1co1sequencing AT hermelindohernandeztorres identificationofencarsiaforsterhymenopteraaphelinidaespeciesfromnortheasternmexicobycytochromecoxidasesubunit1co1sequencing |