Dysregulated Circulating Dendritic Cell Function in Ulcerative Colitis Is Partially Restored by Probiotic Strain Lactobacillus casei Shirota
Background. Dendritic cells regulate immune responses to microbial products and play a key role in ulcerative colitis (UC) pathology. We determined the immunomodulatory effects of probiotic strain Lactobacillus casei Shirota (LcS) on human DC from healthy controls and active UC patients....
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| Format: | Article |
| Language: | English |
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Wiley
2013-01-01
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| Series: | Mediators of Inflammation |
| Online Access: | http://dx.doi.org/10.1155/2013/573576 |
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| author | Elizabeth R. Mann Jialu You Verena Horneffer-van der Sluis David Bernardo Hafid Omar Al-Hassi Jon Landy Simon T. Peake Linda V. Thomas Cheng T. Tee Gui Han Lee Ailsa L. Hart Parveen Yaqoob Stella C. Knight |
| author_facet | Elizabeth R. Mann Jialu You Verena Horneffer-van der Sluis David Bernardo Hafid Omar Al-Hassi Jon Landy Simon T. Peake Linda V. Thomas Cheng T. Tee Gui Han Lee Ailsa L. Hart Parveen Yaqoob Stella C. Knight |
| author_sort | Elizabeth R. Mann |
| collection | DOAJ |
| description | Background. Dendritic cells regulate immune responses to microbial products and play a key role in ulcerative colitis (UC) pathology. We determined the immunomodulatory effects of probiotic strain Lactobacillus casei Shirota (LcS) on human DC from healthy controls and active UC patients. Methods. Human blood DC from healthy controls (control-DC) and UC patients (UC-DC) were conditioned with heat-killed LcS and used to stimulate allogeneic T cells in a 5-day mixed leucocyte reaction. Results. UC-DC displayed a reduced stimulatory capacity for T cells (P<0.05) and enhanced expression of skin-homing markers CLA and CCR4 on stimulated T cells (P<0.05) that were negative for gut-homing marker β7. LcS treatment restored the stimulatory capacity of UC-DC, reflecting that of control-DC. LcS treatment conditioned control-DC to induce CLA on T cells in conjunction with β7, generating a multihoming profile, but had no effects on UC-DC. Finally, LcS treatment enhanced DC ability to induce TGFβ production by T cells in controls but not UC patients. Conclusions. We demonstrate a systemic, dysregulated DC function in UC that may account for the propensity of UC patients to develop cutaneous manifestations. LcS has multifunctional immunoregulatory activities depending on the inflammatory state; therapeutic effects reported in UC may be due to promotion of homeostasis. |
| format | Article |
| id | doaj-art-bd54172861994b7ebf1f44fceab9a3ab |
| institution | Kabale University |
| issn | 0962-9351 1466-1861 |
| language | English |
| publishDate | 2013-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Mediators of Inflammation |
| spelling | doaj-art-bd54172861994b7ebf1f44fceab9a3ab2025-02-03T05:44:07ZengWileyMediators of Inflammation0962-93511466-18612013-01-01201310.1155/2013/573576573576Dysregulated Circulating Dendritic Cell Function in Ulcerative Colitis Is Partially Restored by Probiotic Strain Lactobacillus casei ShirotaElizabeth R. Mann0Jialu You1Verena Horneffer-van der Sluis2David Bernardo3Hafid Omar Al-Hassi4Jon Landy5Simon T. Peake6Linda V. Thomas7Cheng T. Tee8Gui Han Lee9Ailsa L. Hart10Parveen Yaqoob11Stella C. Knight12Antigen Presentation Research Group, Imperial College London, Northwick Park and St. Mark’s Campus, Level 7W St. Mark’s Hospital, Watford Road, Harrow HA1 3UJ, UKDepartment of Food and Nutritional Sciences, University of Reading, Reading, UKNorthwick Park Institute for Medical Research, Harrow, UKAntigen Presentation Research Group, Imperial College London, Northwick Park and St. Mark’s Campus, Level 7W St. Mark’s Hospital, Watford Road, Harrow HA1 3UJ, UKAntigen Presentation Research Group, Imperial College London, Northwick Park and St. Mark’s Campus, Level 7W St. Mark’s Hospital, Watford Road, Harrow HA1 3UJ, UKAntigen Presentation Research Group, Imperial College London, Northwick Park and St. Mark’s Campus, Level 7W St. Mark’s Hospital, Watford Road, Harrow HA1 3UJ, UKAntigen Presentation Research Group, Imperial College London, Northwick Park and St. Mark’s Campus, Level 7W St. Mark’s Hospital, Watford Road, Harrow HA1 3UJ, UKYakult UK Ltd., West End Road, South Ruislip, UKAntigen Presentation Research Group, Imperial College London, Northwick Park and St. Mark’s Campus, Level 7W St. Mark’s Hospital, Watford Road, Harrow HA1 3UJ, UKAntigen Presentation Research Group, Imperial College London, Northwick Park and St. Mark’s Campus, Level 7W St. Mark’s Hospital, Watford Road, Harrow HA1 3UJ, UKAntigen Presentation Research Group, Imperial College London, Northwick Park and St. Mark’s Campus, Level 7W St. Mark’s Hospital, Watford Road, Harrow HA1 3UJ, UKDepartment of Food and Nutritional Sciences, University of Reading, Reading, UKAntigen Presentation Research Group, Imperial College London, Northwick Park and St. Mark’s Campus, Level 7W St. Mark’s Hospital, Watford Road, Harrow HA1 3UJ, UKBackground. Dendritic cells regulate immune responses to microbial products and play a key role in ulcerative colitis (UC) pathology. We determined the immunomodulatory effects of probiotic strain Lactobacillus casei Shirota (LcS) on human DC from healthy controls and active UC patients. Methods. Human blood DC from healthy controls (control-DC) and UC patients (UC-DC) were conditioned with heat-killed LcS and used to stimulate allogeneic T cells in a 5-day mixed leucocyte reaction. Results. UC-DC displayed a reduced stimulatory capacity for T cells (P<0.05) and enhanced expression of skin-homing markers CLA and CCR4 on stimulated T cells (P<0.05) that were negative for gut-homing marker β7. LcS treatment restored the stimulatory capacity of UC-DC, reflecting that of control-DC. LcS treatment conditioned control-DC to induce CLA on T cells in conjunction with β7, generating a multihoming profile, but had no effects on UC-DC. Finally, LcS treatment enhanced DC ability to induce TGFβ production by T cells in controls but not UC patients. Conclusions. We demonstrate a systemic, dysregulated DC function in UC that may account for the propensity of UC patients to develop cutaneous manifestations. LcS has multifunctional immunoregulatory activities depending on the inflammatory state; therapeutic effects reported in UC may be due to promotion of homeostasis.http://dx.doi.org/10.1155/2013/573576 |
| spellingShingle | Elizabeth R. Mann Jialu You Verena Horneffer-van der Sluis David Bernardo Hafid Omar Al-Hassi Jon Landy Simon T. Peake Linda V. Thomas Cheng T. Tee Gui Han Lee Ailsa L. Hart Parveen Yaqoob Stella C. Knight Dysregulated Circulating Dendritic Cell Function in Ulcerative Colitis Is Partially Restored by Probiotic Strain Lactobacillus casei Shirota Mediators of Inflammation |
| title | Dysregulated Circulating Dendritic Cell Function in Ulcerative Colitis Is Partially Restored by Probiotic Strain Lactobacillus casei Shirota |
| title_full | Dysregulated Circulating Dendritic Cell Function in Ulcerative Colitis Is Partially Restored by Probiotic Strain Lactobacillus casei Shirota |
| title_fullStr | Dysregulated Circulating Dendritic Cell Function in Ulcerative Colitis Is Partially Restored by Probiotic Strain Lactobacillus casei Shirota |
| title_full_unstemmed | Dysregulated Circulating Dendritic Cell Function in Ulcerative Colitis Is Partially Restored by Probiotic Strain Lactobacillus casei Shirota |
| title_short | Dysregulated Circulating Dendritic Cell Function in Ulcerative Colitis Is Partially Restored by Probiotic Strain Lactobacillus casei Shirota |
| title_sort | dysregulated circulating dendritic cell function in ulcerative colitis is partially restored by probiotic strain lactobacillus casei shirota |
| url | http://dx.doi.org/10.1155/2013/573576 |
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