Stability and Efficacy of Live-Attenuated Vibrio harveyi Vaccines Under Different Storage Conditions in Zebrafish (Danio rerio) Models
Graphical Abstract Highlight Research • The LD50 (median lethal dose) of Vibrio parahaemolyticus and harveyi in zebrafish was determined to be 1 x 106 CFU/mL, while for V. alginolyticus it was found to be 1 x 105 CFU/mL. • The LAVh vaccine demonstrated cross-protection against various patho...
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Faculty of Fisheries and Marine Universitas Airlangga
2024-09-01
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| Series: | Jurnal Ilmiah Perikanan dan Kelautan |
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| Online Access: | https://e-journal.unair.ac.id/JIPK/article/view/59794 |
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| author | Mohd Hafiz Ngoo Muhamad-Sofie Aslah Mohamad Mohamad Azzam-Sayuti Mohammad Noor Azmai Amal Mohd Zamri-Saad Md Shirajum Monir Ina Salwany Md Yasin |
| author_facet | Mohd Hafiz Ngoo Muhamad-Sofie Aslah Mohamad Mohamad Azzam-Sayuti Mohammad Noor Azmai Amal Mohd Zamri-Saad Md Shirajum Monir Ina Salwany Md Yasin |
| author_sort | Mohd Hafiz Ngoo Muhamad-Sofie |
| collection | DOAJ |
| description | Graphical Abstract
Highlight Research
• The LD50 (median lethal dose) of Vibrio parahaemolyticus and harveyi in zebrafish was determined to be 1 x 106 CFU/mL, while for V. alginolyticus it was found to be 1 x 105 CFU/mL.
• The LAVh vaccine demonstrated cross-protection against various pathogenic strains of Vibrio, leading to an average of 80% survival rate in vaccinated individuals.
• The analysis of the LAVh vaccine emphasized its versatility, as it can be quickly deployed and stored as a freeze-dried powder.
• The LAVh vaccine can be easily accessible and user-friendly in various aquaculture environments, such as offshore and remote farms.
Abstract
Vibriosis poses a significant threat to marine teleosts, causing substantial losses in the global aquaculture industry. Previous work in our lab led to the development of a live-attenuated V. harveyi vaccine (LAVh) candidate that targets the serine endoprotease gene with a three-point knockout and has shown promise in protecting against vibriosis. However, further investigation is necessary to evaluate the stability and efficacy of its various storage conditions for broader applications. This study aims to determine how well the three different LAVh vaccine storage (fresh, stale, and freeze-dried LAVh) worked against vibriosis. A total of 1000 adult zebrafish (Danio rerio) (mean weight: 0.20±0.5 g) were divided into four groups. Groups 1, 2, and 3 were intraperitoneally injected with different LAVh vaccine storage (fresh, stale, and freeze-dried, respectively), while Group 4 received 0.01 M phosphate-buffered saline (PBS) and served as the unvaccinated control. Fish were monitored for 21 days post-vaccination for safety, stability, efficacy, and antibody analysis. The results showed that a modest dosage of 1 x 104 CFU/mL of LAVh vaccine from all storage conditions provided 80% survival upon intraperitoneal challenge with pathogenic strains of pathogenic V. harveyi, V. alginolyticus, and V. parahaemolyticus. This dosage induced significant antibody production and conferred cross-protection against different Vibrio spp., indicating the LAVh vaccine’s potential for commercial application. The LAVh vaccine demonstrated high effectiveness and suitability for storage as a freeze-dried powder. This study might offer significant insights into practical strategies for reducing vibriosis, especially in aquaculture settings with limited infrastructure. |
| format | Article |
| id | doaj-art-bd515ac43fe34017a6bd987fb24d0a7a |
| institution | Kabale University |
| issn | 2085-5842 2528-0759 |
| language | English |
| publishDate | 2024-09-01 |
| publisher | Faculty of Fisheries and Marine Universitas Airlangga |
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| series | Jurnal Ilmiah Perikanan dan Kelautan |
| spelling | doaj-art-bd515ac43fe34017a6bd987fb24d0a7a2025-02-03T05:30:51ZengFaculty of Fisheries and Marine Universitas AirlanggaJurnal Ilmiah Perikanan dan Kelautan2085-58422528-07592024-09-0116232233557960Stability and Efficacy of Live-Attenuated Vibrio harveyi Vaccines Under Different Storage Conditions in Zebrafish (Danio rerio) ModelsMohd Hafiz Ngoo Muhamad-Sofie0https://orcid.org/0009-0000-9985-5989Aslah Mohamad1https://orcid.org/0000-0001-5973-5747Mohamad Azzam-Sayuti2https://orcid.org/0000-0002-7294-8170Mohammad Noor Azmai Amal3https://orcid.org/0000-0002-7664-9821Mohd Zamri-Saad4https://orcid.org/0000-0002-9013-4883Md Shirajum Monir5https://orcid.org/0000-0002-7255-1085Ina Salwany Md Yasin6https://orcid.org/0000-0003-2905-2915Universiti Putra MalaysiaUniversiti Putra MalaysiaUniversiti Putra MalaysiaUniversiti Putra MalaysiaUniversiti Putra MalaysiaBangladesh Fisheries Research Institute Universiti Putra MalaysiaGraphical Abstract Highlight Research • The LD50 (median lethal dose) of Vibrio parahaemolyticus and harveyi in zebrafish was determined to be 1 x 106 CFU/mL, while for V. alginolyticus it was found to be 1 x 105 CFU/mL. • The LAVh vaccine demonstrated cross-protection against various pathogenic strains of Vibrio, leading to an average of 80% survival rate in vaccinated individuals. • The analysis of the LAVh vaccine emphasized its versatility, as it can be quickly deployed and stored as a freeze-dried powder. • The LAVh vaccine can be easily accessible and user-friendly in various aquaculture environments, such as offshore and remote farms. Abstract Vibriosis poses a significant threat to marine teleosts, causing substantial losses in the global aquaculture industry. Previous work in our lab led to the development of a live-attenuated V. harveyi vaccine (LAVh) candidate that targets the serine endoprotease gene with a three-point knockout and has shown promise in protecting against vibriosis. However, further investigation is necessary to evaluate the stability and efficacy of its various storage conditions for broader applications. This study aims to determine how well the three different LAVh vaccine storage (fresh, stale, and freeze-dried LAVh) worked against vibriosis. A total of 1000 adult zebrafish (Danio rerio) (mean weight: 0.20±0.5 g) were divided into four groups. Groups 1, 2, and 3 were intraperitoneally injected with different LAVh vaccine storage (fresh, stale, and freeze-dried, respectively), while Group 4 received 0.01 M phosphate-buffered saline (PBS) and served as the unvaccinated control. Fish were monitored for 21 days post-vaccination for safety, stability, efficacy, and antibody analysis. The results showed that a modest dosage of 1 x 104 CFU/mL of LAVh vaccine from all storage conditions provided 80% survival upon intraperitoneal challenge with pathogenic strains of pathogenic V. harveyi, V. alginolyticus, and V. parahaemolyticus. This dosage induced significant antibody production and conferred cross-protection against different Vibrio spp., indicating the LAVh vaccine’s potential for commercial application. The LAVh vaccine demonstrated high effectiveness and suitability for storage as a freeze-dried powder. This study might offer significant insights into practical strategies for reducing vibriosis, especially in aquaculture settings with limited infrastructure.https://e-journal.unair.ac.id/JIPK/article/view/59794live-attenuated vaccinevibriosisvibrio spp.freeze-dried vaccine |
| spellingShingle | Mohd Hafiz Ngoo Muhamad-Sofie Aslah Mohamad Mohamad Azzam-Sayuti Mohammad Noor Azmai Amal Mohd Zamri-Saad Md Shirajum Monir Ina Salwany Md Yasin Stability and Efficacy of Live-Attenuated Vibrio harveyi Vaccines Under Different Storage Conditions in Zebrafish (Danio rerio) Models Jurnal Ilmiah Perikanan dan Kelautan live-attenuated vaccine vibriosis vibrio spp. freeze-dried vaccine |
| title | Stability and Efficacy of Live-Attenuated Vibrio harveyi Vaccines Under Different Storage Conditions in Zebrafish (Danio rerio) Models |
| title_full | Stability and Efficacy of Live-Attenuated Vibrio harveyi Vaccines Under Different Storage Conditions in Zebrafish (Danio rerio) Models |
| title_fullStr | Stability and Efficacy of Live-Attenuated Vibrio harveyi Vaccines Under Different Storage Conditions in Zebrafish (Danio rerio) Models |
| title_full_unstemmed | Stability and Efficacy of Live-Attenuated Vibrio harveyi Vaccines Under Different Storage Conditions in Zebrafish (Danio rerio) Models |
| title_short | Stability and Efficacy of Live-Attenuated Vibrio harveyi Vaccines Under Different Storage Conditions in Zebrafish (Danio rerio) Models |
| title_sort | stability and efficacy of live attenuated vibrio harveyi vaccines under different storage conditions in zebrafish danio rerio models |
| topic | live-attenuated vaccine vibriosis vibrio spp. freeze-dried vaccine |
| url | https://e-journal.unair.ac.id/JIPK/article/view/59794 |
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