Argonaute-based dual lateral flow test for SARS-CoV-2 and influenza a virus
The lateral flow test (LFT) is crucial for community screening and early warning of infectious pathogens due to its portability and simplicity. However, antigen-based LFTs have relatively low sensitivity, while nucleic acid-based LFTs are limited in specificity and multiplexing capabilities, thus re...
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| Main Authors: | , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2025-06-01
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| Series: | Sensors and Actuators Reports |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2666053925000396 |
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| Summary: | The lateral flow test (LFT) is crucial for community screening and early warning of infectious pathogens due to its portability and simplicity. However, antigen-based LFTs have relatively low sensitivity, while nucleic acid-based LFTs are limited in specificity and multiplexing capabilities, thus requiring improvements in accuracy and efficiency. In this study, we developed a dual lateral flow assay utilizing a thermophilic Argonaute, enabling the detection of multiple nucleic acids on a single strip with a sensitivity of 3 copies/μL and high specificity. Clinical validation showed 100 % concordance with commercial RT-qPCR results. This method not only preserves the high sensitivity of nucleic acid detection but also provides the benefits of portability, dual-target detection, and visual readout, highlighting its potential for point-of-care applications. |
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| ISSN: | 2666-0539 |