Modulation of Th1/Th2 Immune Responses by Killed Propionibacterium acnes and Its Soluble Polysaccharide Fraction in a Type I Hypersensitivity Murine Model: Induction of Different Activation Status of Antigen-Presenting Cells
Propionibacterium acnes (P. acnes) is a gram-positive anaerobic bacillus present in normal human skin microbiota, which exerts important immunomodulatory effects, when used as heat- or phenol-killed suspensions. We previously demonstrated that heat-killed P. acnes or its soluble polysaccharide (PS),...
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Wiley
2015-01-01
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| Series: | Journal of Immunology Research |
| Online Access: | http://dx.doi.org/10.1155/2015/132083 |
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| author | Carla Cristina Squaiella-Baptistão Daniela Teixeira Juliana Sekeres Mussalem Mayari Eika Ishimura Ieda Maria Longo-Maugéri |
| author_facet | Carla Cristina Squaiella-Baptistão Daniela Teixeira Juliana Sekeres Mussalem Mayari Eika Ishimura Ieda Maria Longo-Maugéri |
| author_sort | Carla Cristina Squaiella-Baptistão |
| collection | DOAJ |
| description | Propionibacterium acnes (P. acnes) is a gram-positive anaerobic bacillus present in normal human skin microbiota, which exerts important immunomodulatory effects, when used as heat- or phenol-killed suspensions. We previously demonstrated that heat-killed P. acnes or its soluble polysaccharide (PS), extracted from the bacterium cell wall, suppressed or potentiated the Th2 response to ovalbumin (OVA) in an immediate hypersensitivity model, depending on the treatment protocol. Herein, we investigated the mechanisms responsible for these effects, using the same model and focusing on the activation status of antigen-presenting cells (APCs). We verified that higher numbers of APCs expressing costimulatory molecules and higher expression levels of these molecules are probably related to potentiation of the Th2 response to OVA induced by P. acnes or PS, while higher expression of toll-like receptors (TLRs) seems to be related to Th2 suppression. In vitro cytokines production in cocultures of dendritic cells and T lymphocytes indicated that P. acnes and PS seem to perform their effects by acting directly on APCs. Our data suggest that P. acnes and PS directly act on APCs, modulating the expression of costimulatory molecules and TLRs, and these differently activated APCs drive distinct T helper patterns to OVA in our model. |
| format | Article |
| id | doaj-art-bd1b143514d54c878439897a825e2dbc |
| institution | Kabale University |
| issn | 2314-8861 2314-7156 |
| language | English |
| publishDate | 2015-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Immunology Research |
| spelling | doaj-art-bd1b143514d54c878439897a825e2dbc2025-02-03T01:07:43ZengWileyJournal of Immunology Research2314-88612314-71562015-01-01201510.1155/2015/132083132083Modulation of Th1/Th2 Immune Responses by Killed Propionibacterium acnes and Its Soluble Polysaccharide Fraction in a Type I Hypersensitivity Murine Model: Induction of Different Activation Status of Antigen-Presenting CellsCarla Cristina Squaiella-Baptistão0Daniela Teixeira1Juliana Sekeres Mussalem2Mayari Eika Ishimura3Ieda Maria Longo-Maugéri4Laboratório de Imunoquímica, Instituto Butantan, Avenida Vital Brazil 1500, Prédio Novo, 2° Andar, Butantã, 05503-900 São Paulo, SP, BrazilDisciplina de Imunologia, Departamento de Microbiologia, Imunologia e Parasitologia, Universidade Federal de São Paulo, Rua Botucatu 862, Edifício de Ciências Biomédicas, 4° Andar, Vila Clementino, 04023-062 São Paulo, SP, BrazilDisciplina de Imunologia, Departamento de Microbiologia, Imunologia e Parasitologia, Universidade Federal de São Paulo, Rua Botucatu 862, Edifício de Ciências Biomédicas, 4° Andar, Vila Clementino, 04023-062 São Paulo, SP, BrazilDisciplina de Imunologia, Departamento de Microbiologia, Imunologia e Parasitologia, Universidade Federal de São Paulo, Rua Botucatu 862, Edifício de Ciências Biomédicas, 4° Andar, Vila Clementino, 04023-062 São Paulo, SP, BrazilDisciplina de Imunologia, Departamento de Microbiologia, Imunologia e Parasitologia, Universidade Federal de São Paulo, Rua Botucatu 862, Edifício de Ciências Biomédicas, 4° Andar, Vila Clementino, 04023-062 São Paulo, SP, BrazilPropionibacterium acnes (P. acnes) is a gram-positive anaerobic bacillus present in normal human skin microbiota, which exerts important immunomodulatory effects, when used as heat- or phenol-killed suspensions. We previously demonstrated that heat-killed P. acnes or its soluble polysaccharide (PS), extracted from the bacterium cell wall, suppressed or potentiated the Th2 response to ovalbumin (OVA) in an immediate hypersensitivity model, depending on the treatment protocol. Herein, we investigated the mechanisms responsible for these effects, using the same model and focusing on the activation status of antigen-presenting cells (APCs). We verified that higher numbers of APCs expressing costimulatory molecules and higher expression levels of these molecules are probably related to potentiation of the Th2 response to OVA induced by P. acnes or PS, while higher expression of toll-like receptors (TLRs) seems to be related to Th2 suppression. In vitro cytokines production in cocultures of dendritic cells and T lymphocytes indicated that P. acnes and PS seem to perform their effects by acting directly on APCs. Our data suggest that P. acnes and PS directly act on APCs, modulating the expression of costimulatory molecules and TLRs, and these differently activated APCs drive distinct T helper patterns to OVA in our model.http://dx.doi.org/10.1155/2015/132083 |
| spellingShingle | Carla Cristina Squaiella-Baptistão Daniela Teixeira Juliana Sekeres Mussalem Mayari Eika Ishimura Ieda Maria Longo-Maugéri Modulation of Th1/Th2 Immune Responses by Killed Propionibacterium acnes and Its Soluble Polysaccharide Fraction in a Type I Hypersensitivity Murine Model: Induction of Different Activation Status of Antigen-Presenting Cells Journal of Immunology Research |
| title | Modulation of Th1/Th2 Immune Responses by Killed Propionibacterium acnes and Its Soluble Polysaccharide Fraction in a Type I Hypersensitivity Murine Model: Induction of Different Activation Status of Antigen-Presenting Cells |
| title_full | Modulation of Th1/Th2 Immune Responses by Killed Propionibacterium acnes and Its Soluble Polysaccharide Fraction in a Type I Hypersensitivity Murine Model: Induction of Different Activation Status of Antigen-Presenting Cells |
| title_fullStr | Modulation of Th1/Th2 Immune Responses by Killed Propionibacterium acnes and Its Soluble Polysaccharide Fraction in a Type I Hypersensitivity Murine Model: Induction of Different Activation Status of Antigen-Presenting Cells |
| title_full_unstemmed | Modulation of Th1/Th2 Immune Responses by Killed Propionibacterium acnes and Its Soluble Polysaccharide Fraction in a Type I Hypersensitivity Murine Model: Induction of Different Activation Status of Antigen-Presenting Cells |
| title_short | Modulation of Th1/Th2 Immune Responses by Killed Propionibacterium acnes and Its Soluble Polysaccharide Fraction in a Type I Hypersensitivity Murine Model: Induction of Different Activation Status of Antigen-Presenting Cells |
| title_sort | modulation of th1 th2 immune responses by killed propionibacterium acnes and its soluble polysaccharide fraction in a type i hypersensitivity murine model induction of different activation status of antigen presenting cells |
| url | http://dx.doi.org/10.1155/2015/132083 |
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