Determination of the Phenolic Composition and Antioxidant Activity of Pear Extracts
The specific HPLC analytical procedure was developed and validated for the determination of phenolic compounds in pear samples of different popular cultivars “Conference,” “Concordia,” “Grabova,” and “Patten.” HPLC mobile phase consisted of 0.05% (v/v) trifluoroacetic acid in water and 100% (v/v) ac...
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| Format: | Article |
| Language: | English |
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Wiley
2017-01-01
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| Series: | Journal of Chemistry |
| Online Access: | http://dx.doi.org/10.1155/2017/7856521 |
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| author | Mindaugas Liaudanskas Kristina Zymonė Jonas Viškelis Almantas Klevinskas Valdimaras Janulis |
| author_facet | Mindaugas Liaudanskas Kristina Zymonė Jonas Viškelis Almantas Klevinskas Valdimaras Janulis |
| author_sort | Mindaugas Liaudanskas |
| collection | DOAJ |
| description | The specific HPLC analytical procedure was developed and validated for the determination of phenolic compounds in pear samples of different popular cultivars “Conference,” “Concordia,” “Grabova,” and “Patten.” HPLC mobile phase consisted of 0.05% (v/v) trifluoroacetic acid in water and 100% (v/v) acetonitrile. The HPLC method was used to identify and confirm the specificity of 8 analytes: chlorogenic acid, rutin, hyperoside, isoquercitrin, isorhamnetin rutinoside, quercitrin, quercitrin malonyl glucoside, and isorhamnetin glucoside. Repeatability % RSD did not exceed 3.87%, and intermediate precision did not exceed 4.63%. The total content of phenolic compounds varied from 0.51±0.001 mg/g (cv. “Concordia”) to 1.11±0.013 mg/g (cv. “Patten”). Chlorogenic acid was the major component in all the tested pear cultivars. The highest amount of chlorogenic acid (0.69±0.033 mg/g) was found in pear samples of the cultivar “Grabova,” and the highest amount of flavonol compounds (1.11±0.013 mg/g) was found in pear samples of the cultivar “Concordia.” |
| format | Article |
| id | doaj-art-bd04704a0d334cd9b1d92b275dc57a24 |
| institution | Kabale University |
| issn | 2090-9063 2090-9071 |
| language | English |
| publishDate | 2017-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Chemistry |
| spelling | doaj-art-bd04704a0d334cd9b1d92b275dc57a242025-02-03T01:23:28ZengWileyJournal of Chemistry2090-90632090-90712017-01-01201710.1155/2017/78565217856521Determination of the Phenolic Composition and Antioxidant Activity of Pear ExtractsMindaugas Liaudanskas0Kristina Zymonė1Jonas Viškelis2Almantas Klevinskas3Valdimaras Janulis4Department of Pharmacognosy, Faculty of Pharmacy, Lithuanian University of Health Sciences, Sukilėlių St. 13, LT-44307 Kaunas, LithuaniaDepartment of Pharmacognosy, Faculty of Pharmacy, Lithuanian University of Health Sciences, Sukilėlių St. 13, LT-44307 Kaunas, LithuaniaInstitute of Horticulture, Lithuanian Research Centre for Agriculture and Forestry, Kauno St. 30, Babtai, LT-54333 Kaunas, LithuaniaDepartment of Pharmacognosy, Faculty of Pharmacy, Lithuanian University of Health Sciences, Sukilėlių St. 13, LT-44307 Kaunas, LithuaniaDepartment of Pharmacognosy, Faculty of Pharmacy, Lithuanian University of Health Sciences, Sukilėlių St. 13, LT-44307 Kaunas, LithuaniaThe specific HPLC analytical procedure was developed and validated for the determination of phenolic compounds in pear samples of different popular cultivars “Conference,” “Concordia,” “Grabova,” and “Patten.” HPLC mobile phase consisted of 0.05% (v/v) trifluoroacetic acid in water and 100% (v/v) acetonitrile. The HPLC method was used to identify and confirm the specificity of 8 analytes: chlorogenic acid, rutin, hyperoside, isoquercitrin, isorhamnetin rutinoside, quercitrin, quercitrin malonyl glucoside, and isorhamnetin glucoside. Repeatability % RSD did not exceed 3.87%, and intermediate precision did not exceed 4.63%. The total content of phenolic compounds varied from 0.51±0.001 mg/g (cv. “Concordia”) to 1.11±0.013 mg/g (cv. “Patten”). Chlorogenic acid was the major component in all the tested pear cultivars. The highest amount of chlorogenic acid (0.69±0.033 mg/g) was found in pear samples of the cultivar “Grabova,” and the highest amount of flavonol compounds (1.11±0.013 mg/g) was found in pear samples of the cultivar “Concordia.”http://dx.doi.org/10.1155/2017/7856521 |
| spellingShingle | Mindaugas Liaudanskas Kristina Zymonė Jonas Viškelis Almantas Klevinskas Valdimaras Janulis Determination of the Phenolic Composition and Antioxidant Activity of Pear Extracts Journal of Chemistry |
| title | Determination of the Phenolic Composition and Antioxidant Activity of Pear Extracts |
| title_full | Determination of the Phenolic Composition and Antioxidant Activity of Pear Extracts |
| title_fullStr | Determination of the Phenolic Composition and Antioxidant Activity of Pear Extracts |
| title_full_unstemmed | Determination of the Phenolic Composition and Antioxidant Activity of Pear Extracts |
| title_short | Determination of the Phenolic Composition and Antioxidant Activity of Pear Extracts |
| title_sort | determination of the phenolic composition and antioxidant activity of pear extracts |
| url | http://dx.doi.org/10.1155/2017/7856521 |
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