Characterization of phenolic compounds and evaluation of anti-diabetic potential in Cannabis sativa L. seeds: In vivo, in vitro, and in silico studies
Moroccan Cannabis sativa L. seeds were investigated for their phenolic profile and antidiabetic potential. Ultra-high-performance liquid chromatography with diode array detection and electrospray ionization mass spectrometry analysis revealed a rich phenolic composition, including benzoic acid, cann...
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| Format: | Article |
| Language: | English |
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De Gruyter
2024-12-01
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| Series: | Open Life Sciences |
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| Online Access: | https://doi.org/10.1515/biol-2022-1024 |
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| author | El-Mernissi Rafik El Menyiy Naoual Metouekel Amira Zouhri Aziz El-Mernissi Yahya Siddique Farhan Nadeem Sumaira Amhamdi Hassan Abboussi Oualid Alsahli Abdulaziz Abdullah Bourhia Mohammed Dauelbait Musaab Shazly Gamal A. Hajji Lhoussain |
| author_facet | El-Mernissi Rafik El Menyiy Naoual Metouekel Amira Zouhri Aziz El-Mernissi Yahya Siddique Farhan Nadeem Sumaira Amhamdi Hassan Abboussi Oualid Alsahli Abdulaziz Abdullah Bourhia Mohammed Dauelbait Musaab Shazly Gamal A. Hajji Lhoussain |
| author_sort | El-Mernissi Rafik |
| collection | DOAJ |
| description | Moroccan Cannabis sativa L. seeds were investigated for their phenolic profile and antidiabetic potential. Ultra-high-performance liquid chromatography with diode array detection and electrospray ionization mass spectrometry analysis revealed a rich phenolic composition, including benzoic acid, cannabisin B, genistein, and epicatechin. In vitro, the seed extract exhibited potent α-amylase inhibitory activity (half-maximal inhibitory concentration = 25.02 ± 4.03 μg/mL). In vivo studies in diabetic rats demonstrated significant hypoglycemic, hypolipidemic, hepatoprotective, and nephroprotective effects. Molecular docking studies further supported these findings, revealing strong interactions between identified phenolic and the α-amylase enzyme. These results highlight the potential of C. sativa seeds as a natural source of bioactive compounds for diabetes management. |
| format | Article |
| id | doaj-art-bce5aaf305e840528dee52257a46dda8 |
| institution | Kabale University |
| issn | 2391-5412 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | De Gruyter |
| record_format | Article |
| series | Open Life Sciences |
| spelling | doaj-art-bce5aaf305e840528dee52257a46dda82025-01-20T11:08:14ZengDe GruyterOpen Life Sciences2391-54122024-12-011916314410.1515/biol-2022-1024Characterization of phenolic compounds and evaluation of anti-diabetic potential in Cannabis sativa L. seeds: In vivo, in vitro, and in silico studiesEl-Mernissi Rafik0El Menyiy Naoual1Metouekel Amira2Zouhri Aziz3El-Mernissi Yahya4Siddique Farhan5Nadeem Sumaira6Amhamdi Hassan7Abboussi Oualid8Alsahli Abdulaziz Abdullah9Bourhia Mohammed10Dauelbait Musaab11Shazly Gamal A.12Hajji Lhoussain13Bioactive and Environmental Health Laboratory, Faculty of Sciences, Moulay Ismail University, Meknes, B.P. 11201, MoroccoLaboratory of Pharmacology, National Agency for Medicinal and Aromatic Plants, Taounate, 34025, MoroccoUniversity of Technology of Compiègne, EA 4297 TIMR, 60205Compiègne Cedex, FranceBioactive and Environmental Health Laboratory, Faculty of Sciences, Moulay Ismail University, Meknes, B.P. 11201, MoroccoApplied Chemistry Research Unit, Faculty of Science and Techniques, Abdelmalek Essaadi University, Al-Hoceima, Tetouan, MoroccoSchool of Pharmaceutical Science and Technology, Tianjin University, Tianjin, P.R. ChinaDepartment of Pharmacy, The Women University, Multan, 60800, PakistanApplied Chemistry Research Unit, Faculty of Science and Techniques, Abdelmalek Essaadi University, Al-Hoceima, Tetouan, MoroccoPhysiology and Physiopathology Team, Faculty of Sciences, Genomic of Human Pathologies Research Centre, Mohammed V University, Rabat, MoroccoDepartment of Botany and Microbiology, College of Science, King Saud University, Riyadh11451, Saudi ArabiaLaboratory of Biotechnology and Natural Resources Valorization, Faculty of Sciences, Ibn Zohr University, 80060, Agadir, MoroccoUniversity of Bahr el Ghazal, Freedom Street, Wau, 91113, South SudanDepartment of Pharmaceutics, College of Pharmacy, King Saud University, Riyadh11451, Saudi ArabiaBioactive and Environmental Health Laboratory, Faculty of Sciences, Moulay Ismail University, Meknes, B.P. 11201, MoroccoMoroccan Cannabis sativa L. seeds were investigated for their phenolic profile and antidiabetic potential. Ultra-high-performance liquid chromatography with diode array detection and electrospray ionization mass spectrometry analysis revealed a rich phenolic composition, including benzoic acid, cannabisin B, genistein, and epicatechin. In vitro, the seed extract exhibited potent α-amylase inhibitory activity (half-maximal inhibitory concentration = 25.02 ± 4.03 μg/mL). In vivo studies in diabetic rats demonstrated significant hypoglycemic, hypolipidemic, hepatoprotective, and nephroprotective effects. Molecular docking studies further supported these findings, revealing strong interactions between identified phenolic and the α-amylase enzyme. These results highlight the potential of C. sativa seeds as a natural source of bioactive compounds for diabetes management.https://doi.org/10.1515/biol-2022-1024medicinal plantnatural productsbioactive compoundsnutraceuticalsbiological properties |
| spellingShingle | El-Mernissi Rafik El Menyiy Naoual Metouekel Amira Zouhri Aziz El-Mernissi Yahya Siddique Farhan Nadeem Sumaira Amhamdi Hassan Abboussi Oualid Alsahli Abdulaziz Abdullah Bourhia Mohammed Dauelbait Musaab Shazly Gamal A. Hajji Lhoussain Characterization of phenolic compounds and evaluation of anti-diabetic potential in Cannabis sativa L. seeds: In vivo, in vitro, and in silico studies Open Life Sciences medicinal plant natural products bioactive compounds nutraceuticals biological properties |
| title | Characterization of phenolic compounds and evaluation of anti-diabetic potential in Cannabis sativa L. seeds: In vivo, in vitro, and in silico studies |
| title_full | Characterization of phenolic compounds and evaluation of anti-diabetic potential in Cannabis sativa L. seeds: In vivo, in vitro, and in silico studies |
| title_fullStr | Characterization of phenolic compounds and evaluation of anti-diabetic potential in Cannabis sativa L. seeds: In vivo, in vitro, and in silico studies |
| title_full_unstemmed | Characterization of phenolic compounds and evaluation of anti-diabetic potential in Cannabis sativa L. seeds: In vivo, in vitro, and in silico studies |
| title_short | Characterization of phenolic compounds and evaluation of anti-diabetic potential in Cannabis sativa L. seeds: In vivo, in vitro, and in silico studies |
| title_sort | characterization of phenolic compounds and evaluation of anti diabetic potential in cannabis sativa l seeds in vivo in vitro and in silico studies |
| topic | medicinal plant natural products bioactive compounds nutraceuticals biological properties |
| url | https://doi.org/10.1515/biol-2022-1024 |
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