Mutational Analysis of Colistin-Resistant <i>Pseudomonas aeruginosa</i> Isolates: From Genomic Background to Antibiotic Resistance
This study analyzed eleven isolates of colistin-resistant <i>Pseudomonas aeruginosa</i>, originating from Portugal and Taiwan, which are associated with various pathologies. The results revealed significant genetic diversity among the isolates, with each exhibiting a distinct genetic pro...
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2025-04-01
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| author | Telma De Sousa Hsin-Yao Wang Ting-Wei Lin Manuela Caniça Miguel J. N. Ramos Daniela Santos Catarina Silva Sónia Saraiva Racha Beyrouthy Richard Bonnet Michel Hébraud Gilberto Igrejas Patrícia Poeta |
| author_facet | Telma De Sousa Hsin-Yao Wang Ting-Wei Lin Manuela Caniça Miguel J. N. Ramos Daniela Santos Catarina Silva Sónia Saraiva Racha Beyrouthy Richard Bonnet Michel Hébraud Gilberto Igrejas Patrícia Poeta |
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| description | This study analyzed eleven isolates of colistin-resistant <i>Pseudomonas aeruginosa</i>, originating from Portugal and Taiwan, which are associated with various pathologies. The results revealed significant genetic diversity among the isolates, with each exhibiting a distinct genetic profile. A prevalence of sequence type ST235 was observed, characterizing it as a high-risk clone, and serotyping indicated a predominance of type O11, associated with chronic respiratory infections in cystic fibrosis (CF) patients. The phylogenetic analysis demonstrated genetic diversity among the isolates, with distinct clades and complex evolutionary relationships. Additionally, transposable elements such as Tn3 and IS6 were identified in all isolates, highlighting their importance in the mobility of antibiotic resistance genes. An analysis of antimicrobial resistance profiles revealed pan-drug resistance in all isolates, with a high prevalence of genes conferring resistance to β-lactams and aminoglycosides. Furthermore, additional analyses revealed mutations in regulatory networks and specific loci previously implicated in colistin resistance, such as <i>pmrA</i>, <i>cprS</i>, <i>phoO</i>, and others, suggesting a possible contribution to the observed resistant phenotype. This study has a strong impact because it not only reveals the genetic diversity and resistance mechanisms in <i>P. aeruginosa</i> but also identifies mutations in regulatory genes associated with colistin resistance. |
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| issn | 2076-0817 |
| language | English |
| publishDate | 2025-04-01 |
| publisher | MDPI AG |
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| series | Pathogens |
| spelling | doaj-art-bccd7835c4ed45cf8ef695601c9fbf962025-08-20T02:28:18ZengMDPI AGPathogens2076-08172025-04-0114438710.3390/pathogens14040387Mutational Analysis of Colistin-Resistant <i>Pseudomonas aeruginosa</i> Isolates: From Genomic Background to Antibiotic ResistanceTelma De Sousa0Hsin-Yao Wang1Ting-Wei Lin2Manuela Caniça3Miguel J. N. Ramos4Daniela Santos5Catarina Silva6Sónia Saraiva7Racha Beyrouthy8Richard Bonnet9Michel Hébraud10Gilberto Igrejas11Patrícia Poeta12MicroART—Antibiotic Resistance Team, Department of Veterinary Sciences, University of Trás-os-Montes and Alto Douro, 5000-801 Vila Real, PortugalDepartment of Laboratory Medicine, Chang Gung Memorial Hospital at Linkou, Taoyuan 333, TaiwanDepartment of Laboratory Medicine, Chang Gung Memorial Hospital at Linkou, Taoyuan 333, TaiwanNational Reference Laboratory of Antibiotic Resistance and Healthcare Associated Infections, Department of Infectious Diseases, National Institute of Health Dr. Ricardo Jorge, 1649-016 Lisbon, PortugalNational Reference Laboratory of Antibiotic Resistance and Healthcare Associated Infections, Department of Infectious Diseases, National Institute of Health Dr. Ricardo Jorge, 1649-016 Lisbon, PortugalNational Reference Laboratory of Antibiotic Resistance and Healthcare Associated Infections, Department of Infectious Diseases, National Institute of Health Dr. Ricardo Jorge, 1649-016 Lisbon, PortugalMicroART—Antibiotic Resistance Team, Department of Veterinary Sciences, University of Trás-os-Montes and Alto Douro, 5000-801 Vila Real, PortugalMicroART—Antibiotic Resistance Team, Department of Veterinary Sciences, University of Trás-os-Montes and Alto Douro, 5000-801 Vila Real, PortugalInstitut National de la Santé et de la Recherche Médicale, (UMR1071), Institute National de la Recherche Agronomique (USC-2018), Université Clermont Auvergne, 63000 Clermont-Ferrand, FranceInstitut National de la Santé et de la Recherche Médicale, (UMR1071), Institute National de la Recherche Agronomique (USC-2018), Université Clermont Auvergne, 63000 Clermont-Ferrand, FranceINRAE, Université Clermont Auvergne, UMR Microbiologie Environnement Digestif Santé (MEDiS), 63122 Saint-Genès-Champanelle, FranceDepartment of Genetics and Biotechnology, University of Trás-os-Montes and Alto Douro, 5000-801 Vila Real, PortugalMicroART—Antibiotic Resistance Team, Department of Veterinary Sciences, University of Trás-os-Montes and Alto Douro, 5000-801 Vila Real, PortugalThis study analyzed eleven isolates of colistin-resistant <i>Pseudomonas aeruginosa</i>, originating from Portugal and Taiwan, which are associated with various pathologies. The results revealed significant genetic diversity among the isolates, with each exhibiting a distinct genetic profile. A prevalence of sequence type ST235 was observed, characterizing it as a high-risk clone, and serotyping indicated a predominance of type O11, associated with chronic respiratory infections in cystic fibrosis (CF) patients. The phylogenetic analysis demonstrated genetic diversity among the isolates, with distinct clades and complex evolutionary relationships. Additionally, transposable elements such as Tn3 and IS6 were identified in all isolates, highlighting their importance in the mobility of antibiotic resistance genes. An analysis of antimicrobial resistance profiles revealed pan-drug resistance in all isolates, with a high prevalence of genes conferring resistance to β-lactams and aminoglycosides. Furthermore, additional analyses revealed mutations in regulatory networks and specific loci previously implicated in colistin resistance, such as <i>pmrA</i>, <i>cprS</i>, <i>phoO</i>, and others, suggesting a possible contribution to the observed resistant phenotype. This study has a strong impact because it not only reveals the genetic diversity and resistance mechanisms in <i>P. aeruginosa</i> but also identifies mutations in regulatory genes associated with colistin resistance.https://www.mdpi.com/2076-0817/14/4/387<i>Pseudomonas aeruginosa</i>colistin resistanceWGSmutationsantibiotic resistance |
| spellingShingle | Telma De Sousa Hsin-Yao Wang Ting-Wei Lin Manuela Caniça Miguel J. N. Ramos Daniela Santos Catarina Silva Sónia Saraiva Racha Beyrouthy Richard Bonnet Michel Hébraud Gilberto Igrejas Patrícia Poeta Mutational Analysis of Colistin-Resistant <i>Pseudomonas aeruginosa</i> Isolates: From Genomic Background to Antibiotic Resistance Pathogens <i>Pseudomonas aeruginosa</i> colistin resistance WGS mutations antibiotic resistance |
| title | Mutational Analysis of Colistin-Resistant <i>Pseudomonas aeruginosa</i> Isolates: From Genomic Background to Antibiotic Resistance |
| title_full | Mutational Analysis of Colistin-Resistant <i>Pseudomonas aeruginosa</i> Isolates: From Genomic Background to Antibiotic Resistance |
| title_fullStr | Mutational Analysis of Colistin-Resistant <i>Pseudomonas aeruginosa</i> Isolates: From Genomic Background to Antibiotic Resistance |
| title_full_unstemmed | Mutational Analysis of Colistin-Resistant <i>Pseudomonas aeruginosa</i> Isolates: From Genomic Background to Antibiotic Resistance |
| title_short | Mutational Analysis of Colistin-Resistant <i>Pseudomonas aeruginosa</i> Isolates: From Genomic Background to Antibiotic Resistance |
| title_sort | mutational analysis of colistin resistant i pseudomonas aeruginosa i isolates from genomic background to antibiotic resistance |
| topic | <i>Pseudomonas aeruginosa</i> colistin resistance WGS mutations antibiotic resistance |
| url | https://www.mdpi.com/2076-0817/14/4/387 |
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