Negative Influence of a Long-Term High-Fat Diet on Murine Bone Architecture
A correlation between obesity and bone metabolism is strongly assumed because adipocytes and osteoblasts originate from the same precursor cells and their differentiation is conversely regulated by the same factors. It is controversially discussed if obesity protects bone or leads to loss of bone ma...
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| Format: | Article |
| Language: | English |
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Wiley
2014-01-01
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| Series: | International Journal of Endocrinology |
| Online Access: | http://dx.doi.org/10.1155/2014/318924 |
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| author | Hinrich Fehrendt Thomas Linn Sonja Hartmann Gabor Szalay Christian Heiss Reinhard Schnettler Katrin Susanne Lips |
| author_facet | Hinrich Fehrendt Thomas Linn Sonja Hartmann Gabor Szalay Christian Heiss Reinhard Schnettler Katrin Susanne Lips |
| author_sort | Hinrich Fehrendt |
| collection | DOAJ |
| description | A correlation between obesity and bone metabolism is strongly assumed because adipocytes and osteoblasts originate from the same precursor cells and their differentiation is conversely regulated by the same factors. It is controversially discussed if obesity protects bone or leads to loss of bone mass. Thus, the aim of the present study was to investigate the influence of diet-induced mild obesity (11% increased body weight compared to control) on bone microstructure in mice. Four-week-old male C57BL/6J mice received a high-fat diet (HFD, 60% kcal from fat) and were analyzed by means of dual X-ray absorptiometry, histological methods, real-time RT-PCR, and transmission electron microscopy in comparison to control animals (10% kcal from fat). The cancellous bone mass, collagen 1α1 expression, amount of osteoid, and cohesion of cells via cell-to-cell contacts decreased in HFD mice whereas the bone mineral density and the amount of osteoblasts and osteoclasts were not modified. The amount of apoptotic osteocytes was increased in HFD mice in comparison to controls. We conclude that moderately increased body weight does not protect bone architecture from age-dependent degeneration. By contrast, bone microstructure is negatively affected and reduced maintenance of cell-cell contacts may be one of the underlying mechanisms. |
| format | Article |
| id | doaj-art-bcc3c2319b5e44ef863a214867fb3d8c |
| institution | Kabale University |
| issn | 1687-8337 1687-8345 |
| language | English |
| publishDate | 2014-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | International Journal of Endocrinology |
| spelling | doaj-art-bcc3c2319b5e44ef863a214867fb3d8c2025-02-03T06:44:44ZengWileyInternational Journal of Endocrinology1687-83371687-83452014-01-01201410.1155/2014/318924318924Negative Influence of a Long-Term High-Fat Diet on Murine Bone ArchitectureHinrich Fehrendt0Thomas Linn1Sonja Hartmann2Gabor Szalay3Christian Heiss4Reinhard Schnettler5Katrin Susanne Lips6Laboratory for Experimental Trauma Surgery, Justus Liebig University Giessen, Kerkraderstrasse 9, 35394 Giessen, GermanyClinical Research Unit, Medical Clinic and Polyclinic 3, Justus Liebig University Giessen, Klinikstrasse 33, 35392 Giessen, GermanyLaboratory for Experimental Trauma Surgery, Justus Liebig University Giessen, Kerkraderstrasse 9, 35394 Giessen, GermanyDepartment of Trauma Surgery, University Hospital of Giessen-Marburg, Rudolf-Buchheim-Strasse 7, 35392 Giessen, GermanyDepartment of Trauma Surgery, University Hospital of Giessen-Marburg, Rudolf-Buchheim-Strasse 7, 35392 Giessen, GermanyLaboratory for Experimental Trauma Surgery, Justus Liebig University Giessen, Kerkraderstrasse 9, 35394 Giessen, GermanyLaboratory for Experimental Trauma Surgery, Justus Liebig University Giessen, Kerkraderstrasse 9, 35394 Giessen, GermanyA correlation between obesity and bone metabolism is strongly assumed because adipocytes and osteoblasts originate from the same precursor cells and their differentiation is conversely regulated by the same factors. It is controversially discussed if obesity protects bone or leads to loss of bone mass. Thus, the aim of the present study was to investigate the influence of diet-induced mild obesity (11% increased body weight compared to control) on bone microstructure in mice. Four-week-old male C57BL/6J mice received a high-fat diet (HFD, 60% kcal from fat) and were analyzed by means of dual X-ray absorptiometry, histological methods, real-time RT-PCR, and transmission electron microscopy in comparison to control animals (10% kcal from fat). The cancellous bone mass, collagen 1α1 expression, amount of osteoid, and cohesion of cells via cell-to-cell contacts decreased in HFD mice whereas the bone mineral density and the amount of osteoblasts and osteoclasts were not modified. The amount of apoptotic osteocytes was increased in HFD mice in comparison to controls. We conclude that moderately increased body weight does not protect bone architecture from age-dependent degeneration. By contrast, bone microstructure is negatively affected and reduced maintenance of cell-cell contacts may be one of the underlying mechanisms.http://dx.doi.org/10.1155/2014/318924 |
| spellingShingle | Hinrich Fehrendt Thomas Linn Sonja Hartmann Gabor Szalay Christian Heiss Reinhard Schnettler Katrin Susanne Lips Negative Influence of a Long-Term High-Fat Diet on Murine Bone Architecture International Journal of Endocrinology |
| title | Negative Influence of a Long-Term High-Fat Diet on Murine Bone Architecture |
| title_full | Negative Influence of a Long-Term High-Fat Diet on Murine Bone Architecture |
| title_fullStr | Negative Influence of a Long-Term High-Fat Diet on Murine Bone Architecture |
| title_full_unstemmed | Negative Influence of a Long-Term High-Fat Diet on Murine Bone Architecture |
| title_short | Negative Influence of a Long-Term High-Fat Diet on Murine Bone Architecture |
| title_sort | negative influence of a long term high fat diet on murine bone architecture |
| url | http://dx.doi.org/10.1155/2014/318924 |
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